Cy3B™: Improving the performance of cyanine dyes

Journal of Fluorescence

Volumn 14, Issue 2, 2004, Pages 145-150

  Cooper, Michael ^a   Ebner, Andreas ^b   Briggs, Mark ^a   Burrows, Miles ^a   Gardner, Nicholas ^a   Richardson, Robert ^a   West, Richard ^a  

^a GENERAL ELECTRIC COMPANY   (United States)

^b JOHANNES KEPLER UNIVERSITY LINZ   (Austria)

Author keywords

Bioconjugation; Cy3B; CyDye; Fluorescence

Indexed keywords

ANTIBODIES; BIOASSAY; FLUORESCENCE; LABELING; LIGHT ABSORPTION; MOLECULAR WEIGHT; PH EFFECTS; PHYSIOLOGY; SCREENING; SOLUBILITY;

BIOCONJUGATION; CY3B; CYDYE; EXTINCTION COEFFICIENTS;

DYES;

BENZENESULFONIC ACID DERIVATIVE; CARBOCYANINE; CY3B N HYDROXYSUCCINIMIDE ESTER; CY3B N-HYDROXYSUCCINIMIDE ESTER; CYANINE DYE 3; FLUORESCENT DYE; IMMUNOGLOBULIN G;

ARTICLE; CHEMISTRY; COMPARATIVE STUDY; FLUORESCENCE; SYNTHESIS;

BENZENESULFONATES; CARBOCYANINES; FLUORESCENCE; FLUORESCENT DYES; IMMUNOGLOBULIN G;

EID: 3543051291     PISSN: 10530509     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:JOFL.0000016286.62641.59     Document Type: Conference Paper

References (19)

1. A. J. Pope, H. M. Ulrich, and K. J. Moore (1999). Homogeneous fluorescence readouts for miniaturized high-throughput screening: Theory and practice. Drug Discov. Today 4(8), 350-362.
2. P. Ramm (1999). Imaging systems in assay screening. Drug Discov. Today 4(9), 401-410.
3. P. Ramm and N. Thomas (2003). Technological advances in high-throughput screening. Image-based screening of signal transduction assays. Sci. STKE 2003, p. 14.
4. J. Masino, S. G. Jones, and J. A. Alcock (2002). in Poster Presented at IBC's 7th Annual Drug Discovery Technology™ World Congress, Boston, USA, 2002.
5. T. K. Garyantes (2002). 1536-well assay plates: When do they make sense? Drug Discov. Today 7(9), 489-490.
6. R. B. Mujumdar, L. A. Ernst, S. R. Mujumdar, C. J. Lewis, and A. S. Waggoner (1993). Cyanine dye labelling reagents: Sulfoindocyanine succinimidyl esters. Bioconjug. Chem. 4, 105-111.
7. M. W. Wessendorf and T. C. Brelje (1992). Which fluor is the brightest. A comparison of staining obtained using fluorescein, TMR, rhodamine, Texas Red and Cy3. Histochemistry 98, 81-85.
8. H. J. Gruber, C. D. Hahn, G. Kada, C. K. Riener, G. S. Harms, W. Ahrer, T. Dax, and G. H. G. Knaus (2000). Anomalous fluorescence enhancement of Cy3 and Cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and non-covalent binding to Avidin. Bioconjug. Chem. 11(5), 696-704.
9. A. S. Waggoner and R. Mujumdar (2001). Rigidized Trimethine Cyanine Dyes, U.S. Patent 6133445A.
10. V. Oi, A. N. Glazer, and L. Stryer (1982). Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J. Cell Biol. 93, 981-986.
11. E. Trinquet, F. Maurin, H. Bazin, and G. Mathis. (2003). in Poster Presentation at MAP Conference, Prague August 24th-27th, 2003.
12. D. F. O'Brien, T. M. Kelly, and L. F. Costa (1974). Excited state Properties of some Carbocyanine dyes and the energy transfer mechanism of spectral sensitisation. Photogr. Sci. Eng. 18(1), 76-84.
13. J. R. Lakowicz (1999). Principles of Fluorescence Spectroscopy, 2nd ed., Kluwer Academic, New York, p. 10.
14. M. Fischer and J. Georges (1996). Fluorescence quantum yield of rhodamine 6G in ethanol as a function of concentration using thermal lens spectrometry. Chem. Phys. Lett. 260, 115-118.
15. B. Wieb Van Der Meer, G. Coker III, and S.-Y. Simon Chen (1994). Resonance Energy Transfer, Theory and Data, VCH publishers, New York.
16. R. Sjöback, J. Nygren, and M. Kubista (1998). Characterization of fluorescein-oligonucleotide conjugates and measurement of local electrostatic potential. Biopolymers 46, 445-453.
17. H. Karsilayan, I. Hemmila, H. Takalo, A. Toivonen, K. Pettersson, T. Lovgren, and V.-M. Mukkala (1997). Influence of coupling method on the luminescence properties, coupling efficiency, and binding affinity of antibodies labeled with Europium(III) chelates. Bioconjug. Chem. 8(1), 71-75.
18. S. Turconi, K. Shea, S. Ashman, K. Fantom, D. L. Earnshaw, R. P. Bingham, U. M. Haupts, M. J. B. Brown, and A. J. Pope (2001). Real experiences of uHTS: A prototypic 1536-well fluorescence anisotropy-based uHTS screen and application of well-level quality control procedures. J. Biol Screen. 6(5), 275-290.
19. T. C. Turek-Etienne, E. C. Small, S. C. Soh, T. A. Xin, P. V. Gaitonde, E. B. Barrabee, R. F. Hart, and R. W. Bryant (2003). Evaluation of fluorescent compound interference in 4 fluorescence polarization assays: 2 Kinases, 1 Protsase, and 1 Phosphatase. J. Biol. Screen. 8(2), 176-184.