Miniaturization of fluorescence polarization receptor-binding assays using CyDye-labeled ligands

Journal of Biomolecular Screening

Volumn 8, Issue 4, 2003, Pages 410-420

  Harris, Alison ^a,b   Cox, Sarah ^a   Burns, Dougal ^a   Norey, Christopher ^a  

^a GENERAL ELECTRIC COMPANY   (United States)

^b Maynard Centre ^*

Author keywords

CyDye labeled nonpeptide and peptide ligands; Fluorescence polarization; High throughput screening; Miniaturization of FP receptor assays

Indexed keywords

ATROPINE; BETA 2 ADRENERGIC RECEPTOR; DYE; LIGAND; REAGENT; RECEPTOR; SEROTONIN 3 RECEPTOR; SUBSTANCE P DERIVATIVE; TELENZEPINE; CARBOCYANINE; CELL SURFACE RECEPTOR; CYANINE DYE 3; DRUG DERIVATIVE; FLUORESCENT DYE; MUSCARINIC RECEPTOR; NEUROKININ 1 RECEPTOR; NEUROKININ A; PEPTIDE; PIRENZEPINE; SUBSTANCE P;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; ASSAY; BINDING AFFINITY; BINDING COMPETITION; CHEMICAL LABELING; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG RECEPTOR BINDING; DRUG TARGETING; FLUORESCENCE POLARIZATION; GENE EXPRESSION; HIGH THROUGHPUT SCREENING; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; RELIABILITY; ANIMAL; CHO CELL; ELECTRONICS; EVALUATION; HAMSTER; HUMAN; KINETICS; METABOLISM; METHODOLOGY; SENSITIVITY AND SPECIFICITY;

ANIMALS; ATROPINE; BINDING, COMPETITIVE; CARBOCYANINES; CHO CELLS; CRICETINAE; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; HUMANS; KINETICS; MINIATURIZATION; NEUROKININ A; PEPTIDES; PIRENZEPINE; RECEPTORS, CELL SURFACE; RECEPTORS, MUSCARINIC; RECEPTORS, NEUROKININ-1; SENSITIVITY AND SPECIFICITY; SUBSTANCE P;

EID: 1842775922     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057103256319     Document Type: Article

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