Impact of a red-shifted dye label for high throughput fluorescence polarization assays of G protein-coupled receptors

Journal of Biomolecular Screening

Volumn 5, Issue 5, 2000, Pages 329-334

  Banks, Peter ^a   Gosselin, Mylene ^a   Prystay, Linda ^a  

^a Advanced Bioconcept Company   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA INTERMEDIN; DYE; FLUORESCEIN; G PROTEIN COUPLED RECEPTOR; IODINE 125; PEPTIDE HORMONE;

ARTICLE; BINDING AFFINITY; FLUORESCENCE; FLUORESCENCE POLARIZATION; ISOTOPE LABELING; LIGAND BINDING; PRIORITY JOURNAL; RECEPTOR BINDING;

ALPHA-MSH; BINDING, COMPETITIVE; BORON COMPOUNDS; COLOR; DIMETHYL SULFOXIDE; DRUG EVALUATION, PRECLINICAL; FALSE NEGATIVE REACTIONS; FLUORESCEINS; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; HETEROTRIMERIC GTP-BINDING PROTEINS; LIGANDS; PEPTIDES; RECEPTORS, CELL SURFACE; RHODAMINES; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; TARTRAZINE;

EID: 0033778265     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705710000500504     Document Type: Article

References (9)

1. High throughput screening for drug discovery: Continually transitioning into new technology
2. Homogeneous cell- and bead-based assays for high throughput screening using fluorometric microvolume assay technology
3. Microarray technology advances for therapeutic target discovery
4. Fluorescence polarization assays for high throughput screening of G protein-coupled receptors
5. A simple statistical parameter for use in evaluation and validation of high throughput screening assays
6. High throughput fluorescence polarization: A homogeneous alternative to radioligand binding for cell surface receptors