Expanded bed adsorption as a primary recovery step for the isolation of the insulin precursor MI3 process development and scale up

Biotechnology and Bioengineering

Volumn 93, Issue 1, 2006, Pages 14-20

  Brixius, Peter ^a   Mollerup, Inger ^b   Jensen, Ole Elvang ^b   Halfar, Markus ^a   Thömmes, Jörg ^a,c   Kula, Maria Regina ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b NOVO NORDISK A S   (Denmark)

^c BIOGEN   (United States)

Author keywords

Expanded bed adsorption; Insulin; Primary recovery; Process development; Scale up

Indexed keywords

FLUIDIZED BEDS; HYDRODYNAMICS; INSULIN; PH EFFECTS; REACTION KINETICS; YEAST;

EXPANDED BED ADSORPTION; PRIMARY RECOVERY; PROCESS DEVELOPMENT; SCALE UP;

ADSORPTION;

ADSORBENT; INSULIN PRECURSOR MI3; PROINSULIN; UNCLASSIFIED DRUG;

ADSORPTION; ARTICLE; BINDING KINETICS; CATION EXCHANGE; CELL SUSPENSION; CONDUCTANCE; DRUG ISOLATION; DRUG PURIFICATION; EVALUATION; EXPANDED BED ADSORPTION; FLUIDIZATION; FLUIDIZED BED; HYDRODYNAMICS; INSULIN RELEASE; ISOELECTRIC POINT; NONHUMAN; PH; PROTEIN EXPRESSION; RELIABILITY; SACCHAROMYCES CEREVISIAE; SCALE UP;

ADSORPTION; CHROMATOGRAPHY, LIQUID; HUMANS; PROINSULIN; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 30944433166     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20531     Document Type: Article

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