Expression of monomeric insulin precursor in Pichia pastoris and its conversion into monomeric B27 Lys destripeptide insulin by tryptic hydrolysis

Acta Biochimica et Biophysica Sinica

Volumn 37, Issue 4, 2005, Pages 234-240

  Ding, Jin Guo ^a,c   Fei, Jian ^b   Cui, Da Fu ^a   Zhang, You Shang ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b Shanghai Jiao Tong University Affiliated Sixth People s Hospital   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Monomeric B27 Lys destripeptide insulin; Pichia pastoris; Tryptic hydrolysis

Indexed keywords

AMINO ACID; B28 LYSINE B29 PROINSULIN; INSULIN; MONOMER; MONOMERIC B27 LYSINE DESTRIPEPTIDE INSULIN; PROINSULIN; RECOMBINANT HUMAN INSULIN; UNCLASSIFIED DRUG;

AMINO ACID COMPOSITION; ANIMAL EXPERIMENT; ARTICLE; BIOASSAY; CONTROLLED STUDY; CULTURE MEDIUM; DRUG ACTIVITY; DRUG DESIGN; DRUG PURIFICATION; DRUG SYNTHESIS; ELECTROSPRAY MASS SPECTROMETRY; GEL ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; HYDROPHOBICITY; IN VIVO STUDY; INSULIN SYNTHESIS; ION EXCHANGE CHROMATOGRAPHY; MALE; MOUSE; NONHUMAN; PICHIA PASTORIS; PRECURSOR; PROTEIN ENGINEERING; PROTEIN EXPRESSION;

ANIMALS; GENE EXPRESSION REGULATION, FUNGAL; HUMANS; HYDROLYSIS; INSULIN; MALE; MICE; MICE, INBRED ICR; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRYPSIN;

PICHIA PASTORIS;

EID: 21644461075     PISSN: 16729145     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1745-7270.2005.00040.x     Document Type: Article

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