Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeast Pichia pastoris

Journal of Applied Microbiology

Volumn 107, Issue 3, 2009, Pages 954-963

  Zhu, T ^a   Guo, M ^a,b,c   Tang, Z ^a   Zhang, M ^a   Zhuang, Y ^a   Chu, J ^a   Zhang, S ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b JIANGXI AGRICULTURAL UNIVERSITY   (China)

^c EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Gene dosage; Multi copy Pichia pastoris; Physiological performance; Porcine insulin precursor

Indexed keywords

INSULIN; METHANOL; POLYMERASE CHAIN REACTION; STRAIN RATE; YEAST;

COPY NUMBER; GENE DOSAGE; INSULIN PRECURSORS; MULTI COPIES; MULTI-COPY PICHIA PASTORI; P. PASTORIS; PERFORMANCE; PHYSIOLOGICAL PERFORMANCE; PICHIA PASTORIS; PORCINE INSULIN PRECURSOR;

GENE EXPRESSION;

AMPICILLIN; KANAMYCIN; METHANOL; PIG INSULIN;

DOSE-RESPONSE RELATIONSHIP; GENE EXPRESSION; HORMONE; SECRETION; YEAST;

ARTICLE; CONTROLLED STUDY; FUNGAL STRAIN; FUNGUS GROWTH; GENE DOSAGE; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PICHIA PASTORIS; REAL TIME POLYMERASE CHAIN REACTION;

PICHIA PASTORIS; SUS;

EID: 68849130616     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04279.x     Document Type: Article

References (34)

1. Abdelmoula-souissi, S., Rekik, L., Gargouri, A. Mokdad-gargouri, R. (2007) High-level expression of human tumour suppressor P53 in the methylotrophic yeast: Pichia pastoris. Protein Expr Purif 54, 283 288.
2. Bentley, W.E., Mirjalili, N., Andersen, D.C., Davis, R.H. Kompala, D.S. (1990) Plasmid-encoded protein: The principal factor in the "metabolic burden" associated with recombinant bacteria. Biotechnol Bioeng 35, 668 681.
3. Clare, J.J., Rayment, F.B., Ballantine, S.P., Sreekrishna, K. Romanos, M.A. (1991) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene. Bio-Technology 9, 455 460.
4. Cos, O., Serrano, A., Montesinos, J.L., Ferrer, P., Cregg, J.M. Valero, F. (2005) Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures. J Biotechnol 116, 321 335.
5. Curvers, S., Brixius, P., Klauser, T., Thommes, J., Weuster-botz, D., Takors, R. Wandrey, C. (2001) Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. Fermentation. Biotechnol Prog 17, 495 502. (Pubitemid 32532778)
6. Curvers, S., Linneman, J., Klauser, T., Wandrey, C. Takors, R. (2002) Recombinant protein production with Pichia pastoris in continuous fermentation - kinetic analysis of growth and product formation. Eng Life Sci 28, 229 235.
7. Damasceno, L.M., Anderson, K.A., Ritter, G., Cregg, J.M., Old, L.J. Batt, C.A. (2007) Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris. Appl Microbiol Biotechnol 74, 381 389.
8. Gasser, B., Maurer, M., Rautio, J., Sauer, M., Bhattacharyya, A., Saloheimo, M., Penttila, M. Mattanovich, D. (2007) Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions. BMC Genomics 8, 179.
9. Harju, S., Fedosyuk, H. Peterson, K.R. (2004) Rapid isolation of yeast genomic DNA: Bust n' Grab. BMC Biotechnol 4, 8. doi :.
10. Higgins, D.R. Cregg, J.M. (1998) Pichia Protocols. Totowa, NJ : Humana Press.
11. Hohenblum, H., Gasser, B., Maurer, M., Borth, N. Mattanovich, D. (2004) Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris. Biotechnol Bioeng 85, 367 375.
12. Inan, M., Aryasomayajula, D., Sinha, J. Meagher, M.M. (2006) Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase. Biotechnol Bioeng 93, 771 778.
13. Janes, M., Meyhack, B., Zimmermann, W. Hinnen, A. (1990) The influence of GAP promoter variants on hirudin production, average plasmid copy number and cell growth in Saccharomyces cerevisiae. Curr Genet 18, 97 103.
14. Jones, K.L., Kim, S.W. Keasling, J.D. (2000) Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria. Metab Eng 2, 328 338.
15. Kjeldsen, T., Pettersson, A.F. Hach, M. (1999) Secretory expression and characterization of insulin in Pichia pastoris. Biotechnol Appl Biochem 29, 79 86.
16. Kohrer, K. Domdey, H. (1991) Preparation of high molecular weight RNA. Methods Enzymol 194, 398 405.
17. Lee, C., Kim, J., Shin, S.G. Hwang, S. (2006) Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli. J Biotechnol 123, 273 280.
18. Lee, C., Lee, S., Shin, S.G. Hwang, S. (2008) Real-time PCR determination of rRNA gene copy number: absolute and relative quantification assays with Escherichia coli. Appl Microbiol Biotechnol 78, 371 376.
19. Livak, K.J. Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods 25, 402 408.
20. Macauley-patrick, S., Fazenda, M.L., Mcneil, B. Harvey, L.M. (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22, 249 270.
21. Mansur, M., Cabello, C., Hernandez, L., Pais, J., Varas, L., Valdes, J., Terrero, Y., Hidalgo, A. et al. (2005) Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris. Biotechnol Lett 27, 339 345.
22. Minning, S., Serrano, A., Ferrer, P., Sola, C., Schmid, R.D. Valero, F. (2001) Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol 86, 59 70.
23. Pushnova, E.A., Geier, M. Zhu, Y.S. (2000) An easy and accurate agarose gel assay for quantitation of bacterial plasmid copy numbers. Anal Biochem 284, 70 76.
24. Resina, D., Bollok, M., Khatri, N.K., Valero, F., Neubauer, P. Ferrer, P. (2007) Transcriptional response of Pichia pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction. Microb Cell Fact 6, 21.
25. Scorer, C.A., Clare, J.J., Mccombie, W.R., Romanos, M.A. Sreekrishna, K. (1994) Rapid selection using g418 of high copy number transformants of pichia-pastoris for high-level foreign gene-expression. Bio-technology 12, 181 184.
26. Sreekrishna, K., Nelles, L., Potenz, R., Cruze, J., Mazzaferro, P., Fish, W., Fuke, M., Holden, K. et al. (1989) High-level expression, purification, and characterization of recombinant human-tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. Biochemistry 28, 4117 4125.
27. Sreekrishna, K., Brankamp, R.G., Kropp, K.E., Blankenship, D.T., Tsay, J.T., Smith, P.L., Wierschke, J.D., Subramaniam, A. et al. (1997) Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190, 55 62.
28. Travers, K.J., Patil, C.K., Wodicka, L., Lockhart, D.J., Weissman, J.S. Walter, P. (2000) Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation. Cell 101, 249 258.
29. Trinh, L.B., Phue, J.N. Shiloach, J. (2003) Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris. Biotechnol Bioeng 82, 438 444.
30. Vassileva, A., Chugh, D.A., Swaminathan, S. Khanna, N. (2001) Effect of copy number on the expression levels of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. Protein Expres Purif 21, 71 80. (Pubitemid 32614498)
31. Wang, Y., Liang, Z.H., Zhang, Y.S., Yao, S.Y., Xu, Y.G., Tang, Y.H., Zhu, S.Q., Cui, D.F. et al. (2001) Human insulin from a precursor overexpressed in the methylotrophic yeast Pichia pastoris and a simple procedure for purifying the expression product. Biotechnol Bioeng 73, 74 79.
32. Waterham, H.R., Digan, M.E., Koutz, P.J., Lair, S.V. Cregg, J.M. (1997) Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene 186, 37 44.
33. Zhang, W.H., Bevins, M.A., Plantz, B.A., Smith, L.A. Meagher, M.M. (2000) Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol Bioeng 70, 1 8.
34. Zhou, X.S. Zhang, Y.X. (2002) Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate. Biotechnol Lett 24, 1449 1453.