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Volumn 26, Issue 16, 2004, Pages 1269-1272

Improving the expression of mini-proinsulin in Pichia pastoris

Author keywords

AOX promoter; fed batch culture; Pichia pastoris; recombinant human insulin

Indexed keywords

AMMONIUM SULFATE; EDETIC ACID; MINIPROINSULIN; NITROGEN; PROINSULIN; RECOMBINANT PROTEIN;

EID: 6344292280     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000044871.80632.f9     Document Type: Article
Times cited : (17)

References (12)
  • 1
    • 0000022177 scopus 로고
    • The estimation of pepsin with hemoglobin
    • Anson ML, Mirsky AE (1932) The estimation of pepsin with hemoglobin. J. Gen. Physiol. 10: 342-344.
    • (1932) J. Gen. Physiol. , vol.10 , pp. 342-344
    • Anson, M.L.1    Mirsky, A.E.2
  • 2
    • 0029902402 scopus 로고    scopus 로고
    • A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
    • Castellanos-Serra LR, Fernández-Patron C, Hardy L, Huerta V (1996) A procedure for protein elution from reverse-stained polyacrylamide gels applicable at the low picomole level: an alternative route to the preparation of low abundance proteins for microanalysis. Electrophoresis 17: 1564-1572.
    • (1996) Electrophoresis , vol.17 , pp. 1564-1572
    • Castellanos-Serra, L.R.1    Fernández-Patron, C.2    Hardy, L.3    Huerta, V.4
  • 3
    • 0026094226 scopus 로고
    • Production of mouse epidermal growth factor in yeast: High-level secretion using Pichia postons strains containing multiple gene copies
    • Clare JJ, Romanos MA, Rayment FB, Rowedder JE, Smith MA, Payne MM, Sreekrishna K, Henwood CA (1991) Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia postons strains containing multiple gene copies. Gene 105: 205-212.
    • (1991) Gene , vol.105 , pp. 205-212
    • Clare, J.J.1    Romanos, M.A.2    Rayment, F.B.3    Rowedder, J.E.4    Smith, M.A.5    Payne, M.M.6    Sreekrishna, K.7    Henwood, C.A.8
  • 5
    • 2942627936 scopus 로고    scopus 로고
    • Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures
    • Jahic M, Wallberg F, Bollok M, Garcia P, Enfors SO (2003) Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microbial Cell Factories 2: 6-16.
    • (2003) Microbial Cell Factories , vol.2 , pp. 6-16
    • Jahic, M.1    Wallberg, F.2    Bollok, M.3    Garcia, P.4    Enfors, S.O.5
  • 6
    • 0033043522 scopus 로고    scopus 로고
    • Secretory expression and characterization of insulin in Pichia pastoris
    • Kjeldsen T, Pettersson AF, Hach M ( 1999) Secretory expression and characterization of insulin in Pichia pastoris. Biotechnol. Appl. Biochem. 29: 79-86.
    • (1999) Biotechnol. Appl. Biochem. , vol.29 , pp. 79-86
    • Kjeldsen, T.1    Pettersson, A.F.2    Hach, M.3
  • 7
    • 0037566660 scopus 로고    scopus 로고
    • Efficient production by Escherichia coli of recombinant protein using salting-out effect protecting against proteolytic degradation
    • Kobayashi F, Nakamura Y (2003) Efficient production by Escherichia coli of recombinant protein using salting-out effect protecting against proteolytic degradation. Biotechnol. Lett. 25: 779-782.
    • (2003) Biotechnol. Lett. , vol.25 , pp. 779-782
    • Kobayashi, F.1    Nakamura, Y.2
  • 9
    • 0023472472 scopus 로고
    • Tricine-SDS dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
    • Schagger H, Jagow GV (1987) Tricine-SDS dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368-379.
    • (1987) Anal. Biochem. , vol.166 , pp. 368-379
    • Schagger, H.1    Jagow, G.V.2
  • 12
    • 0035810269 scopus 로고    scopus 로고
    • Human insulin from a precursor over-expressed in the methylotrophic yeast Pichia pastoris and a simple procedure for purifying the expression product
    • Wang Y, Liang ZH, Zhang YS, Yao SY, Xu YG, Tang YH, Zhu SQ, Cui DF, Feng YM (2001) Human insulin from a precursor over-expressed in the methylotrophic yeast Pichia pastoris and a simple procedure for purifying the expression product. Biotechnol. Bioeng. 73: 74-79.3
    • (2001) Biotechnol. Bioeng. , vol.73 , pp. 74-79
    • Wang, Y.1    Liang, Z.H.2    Zhang, Y.S.3    Yao, S.Y.4    Xu, Y.G.5    Tang, Y.H.6    Zhu, S.Q.7    Cui, D.F.8    Feng, Y.M.9


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.