Temperature-induced production of recombinant human insulin in high-cell density cultures of recombinant Escherichia coli

Journal of Biotechnology

Volumn 68, Issue 1, 1999, Pages 71-83

  Schmidt, Michael ^a   Babu, Kunnel Raman ^b   Khanna, Navin ^b   Marten, Sabine ^a   Rinas, Ursula ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

Author keywords

High cell density cultivation; Human insulin; Recombinant Escherichia coli; Temperature inducible expression

Indexed keywords

BATCH CELL CULTURE; BIOSYNTHESIS; ESCHERICHIA COLI; INSULIN; METABOLISM;

HIGH-CELL DENSITY CULTIVATION;

GENETIC ENGINEERING;

CARBON; GLUCOSE; HUMAN INSULIN; HYBRID PROTEIN; SYNTHETIC PEPTIDE;

ARTICLE; BACTERIUM CULTURE; BIOMASS; CELL DENSITY; CELL INCLUSION; CULTURE MEDIUM; DRUG SYNTHESIS; ENERGY RESOURCE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; INSULIN SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; RECOMBINANT DNA TECHNOLOGY; RECOMBINANT GENE;

AMINO ACID SEQUENCE; BIOREACTORS; BIOTECHNOLOGY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HUMANS; INSULIN; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0032970060     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00189-8     Document Type: Article

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