Crystallographic analysis of counterion effects on subtilisin enzymatic action in acetonitrile

Journal of the American Chemical Society

Volumn 132, Issue 7, 2010, Pages 2293-2300

  Cianci, Michele ^a   Tomaszewski, Bartlomiej ^b   Helliwell, John R ^c   Halling, Peter J ^b  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION MECHANISMS; ACTIVE SITE; ANOMALOUS DIFFRACTION; CATALYTIC ACTIVITY; CHARGED GROUPS; COUNTER-ION EFFECTS; COUNTERIONS; CROSSLINKED; CRYSTALLOGRAPHIC ANALYSIS; ENZYMATIC ACTION; ENZYMATIC ACTIVITIES; LOW DIELECTRIC; NON-SPECIFIC BINDING; NONAQUEOUS MEDIA; PROTEIN CONFORMATION; PROTEIN DOMAINS; PROTEIN MOLECULES; SERINE PROTEASE; SOFTER X-RAYS; SUBTILISIN; SUBTILISIN CARLSBERG; WATER MOLECULE;

ACETONITRILE; AMINO ACIDS; BINDING ENERGY; BINDING SITES; BIOCHEMISTRY; CESIUM; CESIUM ALLOYS; CRYSTALS; ENZYMES; GENE ENCODING; MOLECULES; ORGANIC SOLVENTS; PHOSPHATASES; POSITIVE IONS;

ENZYME ACTIVITY;

ACETONITRILE; ANION; CATION; CESIUM CHLORIDE; CESIUM ION; CHLORIDE ION; CHOLINE; COUNTERION; PROTEIN; SERINE PROTEINASE; SODIUM ION; SUBTILISIN;

ARTICLE; BINDING SITE; CATALYSIS; CROSS LINKING; CRYSTAL; CRYSTALLIZATION; ELECTRICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HYDRATION; MOLECULE; PROTEIN CONFORMATION; PROTEIN DOMAIN; X RAY CRYSTALLOGRAPHY;

ACETONITRILES; ANIONS; CARBOXYLIC ACIDS; CATALYSIS; CATALYTIC DOMAIN; CATIONS; CESIUM; CHLORIDES; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; STATIC ELECTRICITY; SUBTILISIN;

EID: 77749285787     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja908703c     Document Type: Article

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