Comparison of x-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water

Proceedings of the National Academy of Sciences of the United States of America

Volumn 95, Issue 22, 1998, Pages 12918-12923

  Schmitke, Jennifer L ^a   Stern, Lawrence J ^a   Klibanov, Alexander M ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONITRILE; N CINNAMOYLIMIDAZOLE; ORGANIC SOLVENT; SUBTILISIN; UNCLASSIFIED DRUG; WATER;

ACYLATION; ARTICLE; CATALYSIS; CINNAMON; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME STRUCTURE; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; SOLVATION; X RAY DIFFRACTION;

ACETONITRILES; ACYLATION; AMINO ACID SEQUENCE; BACILLUS; BINDING SITES; CINNAMATES; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; REPRODUCIBILITY OF RESULTS; SOLVENTS; SUBTILISINS; WATER;

CINNAMOMUM VERUM;

EID: 0032573075     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.95.22.12918     Document Type: Article

References (48)

1. Chen, C.-S. & Sih, C. J. (1989) Angew. Chem. Int. Ed. Engl. 28, 695-707.
2. Klibanov, A. M. (1990) Acc. Chem. Res. 23, 114-120.
3. Dordick, J. S. (1992) Biotechnol. Progr. 8, 259-267.
4. Santaniello, E., Ferraboschi, P. & Grisenti, P. (1993) Enzyme Microb. Technol. 15, 367-382.
5. Gutman, A. L. & Shapira, M. (1995) Adv. Biochem. Eng./Biotechnol. 52, 87-128.
6. Koskinen, A. M. P. & Klibanov, A. M., eds. (1996) Enzymatic Reactions in Organic Media (Blackie, London).
7. Wescott, C. R. & Klibanov, A. M. (1994) Biochim. Biophys. Acta 1206, 1-9.
8. Carrea, G., Ottolina, G. & Riva, S. (1995) Trends Biotechnol. 13, 63-70.
9. Ke, T., Wescott, C. R. & Klibanov, A. M. (1996) J. Am. Chem. Soc. 118, 3366-3374.
10. Wescott, C. R., Noritomi, H. & Klibanov, A. M. (1996) J. Am. Chem. Soc. 118, 10365-10370.
11. Halling, P. J. (1994) Enzyme Microb. Technol. 16, 178-206.
12. Schmitke, J. L., Wescott, C. R. & Klibanov, A. M. (1996) J. Am. Chem. Soc. 118, 3360-3365.
13. Margolin, A. L. (1996) Trends Biotechnol. 14, 223-230.
14. Klibanov, A. M. (1997) Trends Biotechnol. 15, 97-101.
15. Fitzpatrick, P. A., Steinmetz, A. C. U., Ringe, D. & Klibanov, A. M. (1993) Proc. Natl. Acad. Sci. USA 90, 8653-8657.
16. Fitzpatrick, P. A., Ringe, D. & Klibanov, A. M. (1994) Biochem. Biophys. Res. Commun. 198, 675-681.
17. Schmitke, J. L., Stern, L. J. & Klibanov, A. M. (1997) Proc. Natl. Acad. Sci. USA 94, 4250-4255.
18. Yennawar, N. H., Yennawar, H. P. & Farber, G. K. (1994) Biochemistry 33, 7326-7336.
19. Yennawar, H. P., Yennawar, N. H. & Farber, G. K. (1995) J. Am. Chem. Soc. 117, 577-585.
20. Allen, K. N., Bellamacina, C. R., Ding, X., Jeffery, C. J., Mattos, C., Petsko, G. A. & Ringe, D. (1996) J. Phys. Chem. 100, 2605-2611.
21. Zaks, A. & Klibanov, A. M. (1988) J. Biol. Chem. 263, 3194-3201.
22. Kanerva, L. T. & Klibanov, A. M. (1989) J. Am. Chem. Soc. 111, 6864-6865.
23. Adams, K. A. H., Chung, S. & Klibanov, A. M. (1990) J. Am. Chem. Soc. 112, 9418-9419.
24. Chatterjee, S. & Russell, A. J. (1992) Biotechnol. Bioeng. 40, 1069-1077.
25. Neidhart, D. J. & Petsko, G. A. (1988) Protein Eng. 2, 271-276.
26. Schonbaum, G. R., Zerner, B. & Bender, M. L. (1961) J. Biol. Chem. 236, 2930-2935.
27. Otwinowski, Z. & Minor, W. (1997) Methods Enzymol. 276, 307-326.
28. Collaborative Computational Project, Number 4 (1994) Acta Crystallogr. D 50, 760-763.
29. Brünger, A. T., Kuriyan, J. & Karplus, M. (1987) Science 35, 458-460.
30. Jones, T. A., Zou, J. Y., Cowan, S. W. & Kjeldgaard, M. (1991) Acta Crystallogr. A 47, 110-119.
31. Brünger, A. T. (1992) Nature (London) 355, 472-475.
32. McRee, D. E. (1993) Practical Protein Crystallography (Academic, San Diego), pp. 226-227.
33. Kleywegt, G. J. (1995) ESF/CCP 4 Newsl. 32.
34. Wierda, D. A., Feng, T. L. & Barron, A. R. (1989) Acta Crystallogr. C 45, 339-341.
35. Laskowski, R. A., MacArthur, M. W., Moss, D. S. & Thornton, J. M. (1993) J. Appl. Crystallogr. 26, 283-291.
36. Luzzati, V. (1952) Acta Crystallogr. 5, 802-810.
37. Kleywegt, G. L. & Jones, T. A. (1994) Structure 2, 1241-1258.
38. Kleywegt, G. L. & Brünger, A. T. (1996) Structure 4, 897-904.
39. Read, R. J. (1986) Acta Crystallogr. A 42, 140-149.
40. Voet, D. & Voet, J. G. (1995) Biochemistry (Wiley, New York), 2nd Ed., pp. 398-400.
41. Partridge, J., Hutcheon, G. A., Moore, B. D. & Hailing, P. J. (1996) J. Am. Chem. Soc. 118, 12874-12877.
42. Connolly, M. L. (1983) Science 221, 709-712.
43. Zheng, Y. & Ornstein, R. L. (1996) J. Am. Chem. Soc. 118, 4175-4180.
44. Toba, S. & Mertz, K. M. (1997) J. Am. Chem. Soc. 119, 9939-9948.
45. Chen, K. & Arnold, F. H. (1993) Proc. Natl. Acad. Sci. USA 90, 5618-5622.
46. Moss, D. E. & Fahrney, D. E. (1978) Biochem. Pharmacol. 27, 2693.
47. Schulze, B. & Klibanov, A. M. (1991) Biotechnol. Bioeng. 38, 1001-1006.
48. Khalaf, N., Govardhan, C. P., Lalonde, J. J., Persichetti, R. A., Wang, Y. & Margolin, A. L. (1996) J. Am. Chem. Soc. 118, 5494-5495.