Studies on crystallization and cross-linking of lipase for biocatalysis

Bioprocess and Biosystems Engineering

Volumn 31, Issue 2, 2008, Pages 87-94

  Rajan, Akhila ^a   Emilia Abraham, T ^a  

^a REGIONAL RESEARCH LABORATORY CSIR   (India)

Author keywords

CLEC lipase; Cross linking; Crystallization; Organic solvent; Thermal stability

Indexed keywords

CROSSLINKING; CRYSTALLIZATION; CYCLODEXTRINS; ORGANIC SOLVENTS; SYNTHESIS (CHEMICAL); THERMODYNAMIC STABILITY;

ETHYL BUTYRATE; IBUPROFEN; REUSE STABILITY;

BIOCATALYSTS;

2 PROPANOL; AMMONIUM SULFATE; BETA CYCLODEXTRIN; GLUTAMIC ACID; GLUTARALDEHYDE; HEXYLENE GLYCOL; HISTIDINE; SERINE; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOCATALYST; BURKHOLDERIA CEPACIA; CROSS LINKING; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVATION; PRIORITY JOURNAL; THERMOSTABILITY;

CATALYSIS; CROSS-LINKING REAGENTS; CRYSTALLIZATION; ENZYME ACTIVATION; ENZYME STABILITY; LIPASE; PARTICLE SIZE; PROTEIN CONFORMATION;

BURKHOLDERIA CEPACIA;

EID: 38649123713     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-007-0149-5     Document Type: Article

References (21)

1. Govardhan CP (1999) Crosslinking of enzymes for improved stability and performance. Curr Opin Biotechnol 10:331-335
2. Margolin AL (1996) Novel crystalline catalysts TIBTECH 14:223-230
3. Jegan Roy J, Emilia Abraham T (2004) Strategies in making cross-linked enzyme crystals. Chem Rev 104:3705
4. Ransac S, Rogalska E, Gargouri Y, Deveer AMTJ, Paltauf F, De Haas GH, Verger R (1990) Stereoselectivity of lipases. I. Hydrolysis of enantiomeric glyceride analogs by gastric and pancreatic lipases, a kinetic study using the monomolecular film technique. J Biol Chem 265:20263-20270
5. Rogalska E, Cudrey C, Ferrato F, Verger R (1993) Stereoselective hydrolysis of triglycerides by animal and microbial lipases. Chirality 5: 24-30
6. Salunkhe DR (1992) Rice. In: World oilseeds: chemistry, technology, and utilization. Van Nostrand Reinhold, New York, pp 424-448
7. Ducret A, Pepin P, Trani M, Lortie R. (1995) Scale-up of the enantioselective reaction for the enzymatic resolution of (R, S)-ibuprofen. Biotechnol Lett 17:1095-1098
8. Ergan F, Trani M, Lortie R (1995) Selective esterification of racemic ibuprofen. Ann N Y Acad Sci 750:228-231
9. Lalonde JJ, Govardhan C, Khalaf N, Martinez AG, Visuri K, Margolin AL (1995) Cross-linked crystals of Candida rugosa lipase: highly efficient catalysts for the resolution of chiral esters. J Am Chem Soc 117:6845-6852
10. Khalaf N, Govardhan CP, Lalonde JJ, Persichetti RA, Wang YF, Margolin AL (1996) Cross-linked enzyme crystals as highly active catalysts in organic solvents. J Am Chem Soc 118:5494-5495
11. Browne JK, McKervey MA, Pitarch M, Russell JA (1998) Enzymatic synthesis of nonracemic inherently chiral calix [4] arenes by lipase-catalysed transesterification. Tetrahedron Lett 39:1787-1790
12. Persichetti RA, Lalonde JJ, Govardhan CP, Khalaf NK, Margolin AL (1996) Candida rugosa lipase: enantioselectivity enhancements in organic solvents. Tetrahedron Lett 37:6507-6510
13. Pepin P, Lortie R (2001) Influence of water activity on the enantioselective esterification of (R, S)-ibuprofen by crosslinked crystals of Candida Antarctica lipase B in organic solvent media. Biotechnol Bioeng 75:559-562
14. M of lipase catalyzed transesterification in organic media. Biotechnol Lett 15:937-942
15. Ottolina G, Carrea G, Riva S, Sartore L, Veronese FM (1992) Effect of the enzyme form on the activity, stability and enantioselectivity of lipoprotein lipase in toluene. Biotechnol Lett 14:947-952
16. Noomrio MH, Khan MY, Dahot MU (1990) Purification and characterization of Cajanus cajan L. seed lipase. In: Proceedings of National Chemical Conference II, pp 139-142
17. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein Measurement with Folin-Phenol reagent. J Biol Chem 193:265-275
18. Ducret A, Trani M, Lortie R (1998) Lipase-catalyzed enantioselective esterification of ibuprofen in organic solvents under controlled water activity. Enzyme Microb Technol 22:212-216
19. Grim MD (2007) In: Kirst HA, Yeh W-K, Zmijewski MJ (eds) Enzyme Technologies for Pharmaceutical and Biotechnological Applications, Marcel Dekker, New York, pp 209-226
20. Marcela A, Eduardo H, Michael AP, Rafael V (2002) Cross-linked crystals of chloroperoxidase. Biochem Biophys Res Commun 295:828
21. Lalonde JJ, Navia MA, Margolin AL (1997) Crosslinked enzyme crystals of lipases as catalysts for kinetic resolution of acids and alcohols. Methods Enzymol 286:443-464