Operational stability of high initial activity protease catalysts in organic solvents

Biotechnology Progress

Volumn 18, Issue 6, 2002, Pages 1455-1457

  Amorim Fernandes, João F ^a   Halling, Peter J ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

SPECIFIC ACTIVITY;

ALCOHOLS; CATALYSTS; CROSSLINKING; CRYSTALS; SOLVENTS;

ENZYMES;

BIOREACTORS; CATALYSIS; CRYSTALLIZATION; ENDOPEPTIDASES; ENZYME STABILITY; KINETICS; SOLVENTS; SUBTILISIN;

EID: 0036860692     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp020098g     Document Type: Article

References (21)

1. Partridge, J.; Halling, P. J.; Moore, B. D. Practical route to high activity enzyme preparations for synthesis in organic media. Chem. Commun. 1998, 7, 841-842.
2. Partridge, J.; Halling, P. J.; Moore, B. D. Solid-state proton/sodium buffers: "Chemical pH stats" for biocatalysis in organic Solvents. J. Chem. Soc., Perkin Trans. 2000, 2, 465-471.
3. Partridge, J.; Hutcheon, G. A.; Moore, B. D.; Halling, P. J. Exploiting hydration hysteresis for high activity of cross-linked subtilisin crystals in acetonitrile. J. Am. Chem. Soc. 1996, 118, 12873-12877.
4. Schmitke, J. L.; Stern, L. J.; Klibanov, A. M. Comparison of X-ray crystal structures of an acyl-enzyme intermediate of subtilisin Carlsberg formed in anhydrous acetonitrile and in water. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 12918-12923.
5. Kimura, Y.; Yoshida, T.; Muraya, K.; Nakanishi, K.; Matsuno, R. Continuous synthesis of a tripeptide by successive condensation and transesterification catalysed by two immobilised proteinases in organic solvent. Agric. Biol. Chem. 1990, 54, 1433-1440.
6. Richards, A. O.; Gill, I. S.; Vulfson, E. N. Continuous enzymatic production of oligopeptides: Synthesis of an enkephalin pentapeptide in a multistage bioreactor. Enzyme Microb. Technol. 1993, 15, 928-935.
7. Nakanishi, K.; Kamikubo, T.; Matsuno, R. Continuous synthesis of N-(benzylocarboxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester with immobilised thermolysin in an organic solvent. Bio/Technology 1985, 3, 459-464.
8. Nakanishi, K.; Kondo, M.; Matsuno, R. Repeated batch and continuous synthesis of N-(benzyloxycarbonyl)-L-phenylalanyl-L-phenylalanine methyl ester with immobilised thermolysin. Appl. Microbiol. Biotechnol. 1988, 28, 229-234.
9. Nakanishi, K.; Matsuno, R. Repeated batch and continuous synthesis of N-(benzyloxycarbonyl)-L-phenylalanyl-L-phenylalanine methyl ester with immobilised thermolysin. Ann. N.Y. Acad. Sci. 1990, 613, 652-655.
10. Nakanishi, K.; Takeuchi, A.; Matsuno, R. Long-term continuous synthesis of aspartame precursor in a column reactor with an immobilised thermolysin. Appl. Microbiol. Biotechnol. 1990, 32, 633-636.
11. Nakanishi, K.; Nagayasu, T. Peptide synthesis in organic solvents with an immobilised enzyme. Biomed. Biochim. Acta 1991, 50, S50-S54.
12. Nagayasu, T.; Miyanaga, M.; Tanaka, T.; Sakiyama, T.; Nakanishi, K. Synthesis of aspartame precursor with an immobilised thermolysin in tert-amyl alcohol. Biotechnol. Bioeng. 1994, 43, 1118-1123.
13. Miyanaga, M.; Tanaka, T:; Sakiyama, T.; Nakanishi, K. Synthesis of aspartame precursor with an immobilised thermolysin in mixed organic solvents. Biotechnol. Bioeng. 1995, 46, 631-635.
14. Persichetti, R. A.; St. Clair, N. L.; Griffith, J. P.; Navia, M. A.; Margolin, A. L. Cross-linked enzyme crystals (CLECs) of thermolysin in the synthesis of peptides. J. Am. Chem. Soc. 1995, 117, 2732-2737.
15. Reslow, M.; Adlercreutz, P.; Mattiasson, B. Organic solvents for bioorganic synthesis. 1. Optimisation of parameters for a chymotrypsin catalysed process. Appl. Microbiol. Biotechnol. 1987, 26, 1-8.
16. Reslow, M.; Adlercreutz, P.; Mattiasson, B. The influence of water on protease-catalysed peptide synthesis in acetonitrile/water mixtures. Eur. J. Biochem. 1988, 177, 313-318.
17. Kise, H.; Hayakawa, A. Immobilisation of proteases to porous chitosan beads and their catalysis for ester and peptide synthesis in organic solvents. Enzyme Microb. Technol. 1991, 13, 584-588.
18. Pirozzi, D.; Halling, P. J. Development of small-size tubular-flow continuous reactors for the analysis of operational stability of enzymes in low-water systems. Biotechnol. Bioeng. 2001, 72, 244-249.
19. Wilcox, P. E. In Methods in Enzymology; Perlman, G. E., Lorando, L., Eds.; Academic Press: London, 1970; Vol. 29, pp 72-73.
20. Sadana, A. Biocatalysis: Fundamentals of Enzymes Deactivation Kinetics; Prentice Hall: New Jersey, 1991.
21. Klibanov, A. M. Improving enzymes by using them in organic solvents. Nature 2001, 409, 214-246.