Current strategies for polypeptide fusion tags removal

Progress in Biochemistry and Biophysics

Volumn 36, Issue 10, 2009, Pages 1364-1669

  Hao, Xie ^a   Cheng, Yang ^a   Li E, Chen ^a  

^a WUHAN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Fusion proteins; Intein; Polypeptide fusion tags; Protease; Tag removal

Indexed keywords

EID: 74549196928     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: 10.3724/SP.J.1206.2009.00160     Document Type: Article

References (39)

1. Xie H, Guo X M, Chen H. Making the most of fusion tags technology in structural characterization of membrane proteins. Mol Biotech, 2009, 42 (2):135-145
2. Waugh D S. Making the most of affinity tags. TRENDS in Biotechnology, 2005, 23 (6):316-320
3. Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Micro Biotech, 2003, 60 (5):523-533
4. Chant A, Kraemer-Pecore C M, Watkin R, et al. Attachment of a histidine tag to the minimal zinc finger protein of the Aspergillus nidulans gene regulatory protein AreA causes a conformational change at the DNA-binding site. Prot Exp Puri, 2005, 39 (2):152-159
5. Cadel S, Gouzy-Darmon C, Petres S, et al. Expression and purification of rat recombinant aminopeptidase B secreted from baculovirus-infected insect cells. Prot Exp Puri, 2004, 36 (1):19-30
6. Goel A, Colcher D, Koo J S, et al. Relative position of the hexahistidine tag effects binding properties of a tumor-associated single-chain Fv construct. Biochim Biophys Acta, 2000, 1523 (1):13-20
7. Arnau J, Lauritzen C, Petersen G E, et al. Current strategies for the use of affinity tags and tag removal for the purifcation of recombinant proteins. Prot Exp Puri, 2006, 48 (1):1-13
8. Fairlie W D, Uboldi A D, De Souza D P, et al. A fusion protein system for the recombinant production of short disulfide-containing peptides. Prot Exp Puri, 2002, 26 (1):171-178
9. Kenig M, Peternel S, Gaberc-Porekar V, et al. Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins. J Chromato A, 2006, 1101(1-2):293-306
10. Simons B L, King M C, Cyr T, et al. Covalent cross-linking of proteins without chemical reagents. Protein Science, 2002, 11 (6):1558-1564
11. Jenny R J, Mann K G, Lundblad R L. A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. Prot Exp Puri, 2003, 31 (1):1-11
12. Shahravan S H, Qu X L, Chan I S, et al. Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tag. Prot Exp Puri, 2008, 59 (2):314-319
13. Yeliseev A, Zoubak L, Gawrisch K. Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor. Prot Exp Puri, 2007, 53 (1):153-163
14. Lorentsen R H, Fynbo C H, Thøgersen H C, et al. Expression, refolding, and purification of recombinant human granzyme B. Prot Exp Puri, 2005, 39 (1):18-26
15. Chatterjee S, Schoepe J, Lohmer S, et al. High level expression and single-step purification of hexahistidine-tagged L-2-hydroxyisocaproate dehydrogenase making use of a versatile expression vector set. Prot Exp Puri, 2005, 39 (2):137-143
16. 4Lys on enterokinase cleavage of a fusion protein. Anal Biochem, 1999, 269 (1):10-16
17. Di Cesare Mannelli L, Pacini A, Toscano A, et al. Gi/o proteins: expression for direct activation enquiry. Prot Exp Puri, 2006, 47 (1):303-310
18. Lin X H, Xu S F, Yang Y P, et al. Purification and characterization of anthranilate synthase component I (TrpE) from Mycobacterium tuberculosis H37Rv. Prot Exp Puri, 2009, 64 (1):8-15
19. Mao H. A self-cleavable sortase fusion for one-step purification of free recombinant proteins. Prot Exp Puri, 2004, 37 (1):253-263
20. Qiagen. TAGZyme™ Handbook for Exoproteolytic Cleavage of N-terminal His tags, 2003
21. Block H, Kubicek J, Labahn J, et al. Production and comprehensive quality control of recombinant human Interleukin-1 beta: A case study for a process development strategy. Prot Exp Puri, 2008, 57 (2):244-254
22. Arnau J, Lauritzen C, Petersen G E, et al. The use of TAGZyme for the efficient removal of n-terminal His-tags. Metho Mol Biol, 2008, 421:229-243
23. Arnau J, Lauritzen C, Pedersen J. Cloning strategy, production and purification of proteins with exopeptidase-cleavable His-tags. Nature Protocols, 2006, 1 (5):2326-2333
24. Ordu E B, Karaguler N G. Improving the purification of NAD (+) - dependent formate dehydrogenase from Candida methylica. Prep Biochem Biotech, 2007, 37 (4):333-341
25. Pedersen J, Lauritzen C, Madsen M T, et al. Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases. Prot Exp Puri, 1999, 15 (3):389-400
26. Frottin F, Martinez A, Peynot P, et al. The proteomics of N-terminal methionine cleavage. Mole Cell Prot, 2006, 5 (12):2336-2349
27. Shao A Y, Meng Q. Chin J Biochem Mole Biol, 2008, 24 (6):512-521
28. Perler F B. InBase, the intein database. Nucl Acid Res, 2002, 30 (1):383-384
29. http://www.neb.com/neb/inteins.html
30. Guo C, Li Z, Shi Y, et al. Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin. Prot Exp Puri, 2004, 37 (2):361-367
31. Mathys S, Evans T C, Chute I C, et al. Characterization of a self-splicing mini-intein and its conversion into autocatalytic N-and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene, 1999, 231(1-2):1-13
32. Xu M Q, Perler F B. The mechanism of protein splicing and its modulation by mutation. EMBO Journal, 1996, 15 (19):5146-5153
33. Humphries H E, Christodoulides M, Heckels J E. Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag. Prot Exp Puri, 2002, 26 (2):243-248
34. Sun Z, Chen J, Yao H, et al. Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli. Prot Exp Puri, 2005, 43 (1):26-32
35. Singleton S F, Simonette R A, Sharma N C, et al. Intein-mediated affinity-fusion purification of the Escherichia coli RecA protein. Prot Exp Puri, 2002, 26 (3):476-488
36. Mee C, Banki M R, Wood D W. Towards the elimination of chromatography in protein purification: expressing proteins engineered to purify themselves. Chem Engin J, 2008, 135(1-2):56-62
37. Peroza E A, Freisinger E. Tris is a non-innocent buffer during intein-mediated protein cleavage. Prot Exp Puri, 2008, 57 (2):217-225
38. Banki M R, Gerngross T U, Wood D W. Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association. Prot Sci, 2005, 14 (6):1387-1395
39. Banki M R, Feng L, Wood D W. Simple bioseparations using self-cleaving elastin-like polypeptide tags. Nat Methods, 2005, 2 (9):659-661