Tris is a non-innocent buffer during intein-mediated protein cleavage

Protein Expression and Purification

Volumn 57, Issue 2, 2008, Pages 217-225

  Peroza, Estevão A ^a   Freisinger, Eva ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Aminolysis; Methionine pathway; Plant metallothionein; Self splicing purification tag; Tris adduct

Indexed keywords

DITHIOTHREITOL; INTEIN; METHIONINE; TROMETAMOL; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; GENE; GENE EXPRESSION; GENETICS; HYDROLYSIS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PROTEIN PROCESSING; PROTEIN SYNTHESIS; WHEAT;

AMINO ACID SEQUENCE; BASE SEQUENCE; DITHIOTHREITOL; GENE EXPRESSION; GENES, PLANT; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; INTEINS; METHIONINE; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; PROTEIN BIOSYNTHESIS; PROTEIN PROCESSING, POST-TRANSLATIONAL; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRITICUM; TROMETHAMINE;

TRITICUM AESTIVUM;

EID: 37349089033     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.10.003     Document Type: Article

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