The use of TAGZyme for the efficient removal of N-terminal his-tags

Methods in Molecular Biology

Volumn 421, Issue , 2007, Pages 229-243

  Arnau, José ^a   Lauritzen, Conni ^a   Petersen, Gitte Ebert ^a   Pedersen, John ^a  

^a NONE

Author keywords

Affinity tag removal; Aminopeptidases; Downstream; Histidine tags; N terminal tag; Processing; Recombinant protein; TAGZyme

Indexed keywords

ENZYME; HISTIDINE; PRIMER DNA;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

AMINO ACID SEQUENCE; BASE SEQUENCE; DNA PRIMERS; ENZYMES; HISTIDINE; MOLECULAR SEQUENCE DATA;

ESCHERICHIA COLI;

EID: 40549118522     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (8)

1. Derewenda, Z.S. (2004) The use of recombinant methods and molecular engineering in protein crystallization. Methods 34, 354-363.
2. Liew, O.W., Ching Chong, J.P., Yandle, T.G. and Brennan, S.O. (2005) Preparation of recombinant, thioredoxin fused N-terminal proCNP: analysis of enterokinase cleavage products reveals new enterokinase cleavage sites. Protein Expr. Purif. 41, 332-340.
3. He, M., Jin, L. and Austen B. (1993) Specificity of factor Xa in the cleavage of fusion proteins. J. Protein Chem. 12, 1-5.
4. Pedersen, J., Lauritzen, C., Madsen, M.T. and Dahl, S.W. (1999) Removal of N-terminal polyhistidine tags from recombinant proteins using engineered aminopeptidases. Protein Expr. Purif 15, 389-400.
5. TAGZyme manual (2003). Available from Qiagen at http://wwwl.qiagen.com/ literature/handbooks/PDF/Protein/Purification/QXP_TAGZyme/1024037_HBQXPT AGZyme_032003.pdf
6. Hirel, P.H., Schmitter, J.M., Dessen, P., Fayat, G. and Blanquet, S. (1989) Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Proc. Natl Acad. Sci. U. S. A. 86, 8247-8251.
7. Dahl, S.W., Slaughter, C., Lauritzen, C., Bateman, R.C., Connerton, I. and Pedersen J. (2000) Carica papaya glutamine cyclotransferase belongs to a novel plant enzyme subfamily: cloning and characterization of the recombinant, enzyme. Protein Expr. Purif. 20, 27-36.
8. Roach, D.R., Bean, A.G., Demangel, C., France, M.P., Briscoe, H. and Britton, W.J. (2002) TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection. J. Immunol. 168, 4620-4627.