Attachment of a histidine tag to the minimal zinc finger protein of the Aspergillus nidulans gene regulatory protein AreA causes a conformational change at the DNA-binding site

Protein Expression and Purification

Volumn 39, Issue 2, 2005, Pages 152-159

  Chant, Alan ^a,c   Kraemer Pecore, Christina M ^c   Watkin, Richard ^b   Kneale, Geoff G ^a  

^a UNIVERSITY OF PORTSMOUTH   (United Kingdom)

^b UNIVERSITY OF VERMONT   (United States)

^c UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

Denaturation; DNA binding domain; Fluorescence; GATA protein; Histidine tag

Indexed keywords

CYSTEINE; FUNGAL PROTEIN; HISTIDINE; THROMBIN; TRYPTOPHAN; UREA; ZINC FINGER PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIDULANS; BINDING SITE; CHEMISTRY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN TERTIARY STRUCTURE; SPECTROFLUOROMETRY;

AMINO ACID SEQUENCE; ASPERGILLUS NIDULANS; BINDING SITES; CYSTEINE; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENETIC VECTORS; HISTIDINE; KINETICS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STRUCTURE, TERTIARY; SPECTROMETRY, FLUORESCENCE; THROMBIN; TRYPTOPHAN; UREA; ZINC FINGERS;

ASPERGILLUS; EMERICELLA NIDULANS;

EID: 11844272620     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.10.017     Document Type: Article

References (27)

1. M.X. Caddick Nitrogen metabolite repression Prog. Ind. Microbiol. 29 1994 323 353
2. M. Merika, and S.H. Orkin DNA-binding specificity of GATA family transcription factors Mol. Cell. Biol. 13 1993 3999 4010
3. B. Kudla, M.X. Caddick, T. Langdon, N.M. Martinez-Rossi, S. Bennett, S. Sibley, R.W. Davis, and H.N. Arst Jr. The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger Eur. J. Biochem. 9 1990 1355 1364
4. P.J. Punt, J. Strauss, R. Smit, J.R. Kinghorn, C.A.M.J.J. van den Hondel, and C. Scazzocchio The intergenic region between the divergently transcribed niiA and niaD genes of Aspergillus nidulans contains multiple NirA binding sites which act bidirectionally Mol. Cell. Biol. 15 1995 5688 5699
5. M.A. Davis, and M.J. Hynes Regulatory genes in Aspergillus nidulans Trends Genet. 5 1989 14 19
6. L.J. Ko, and J.D. Engel DNA-binding specificities of the GATA transcription factor family Mol. Cell. Biol. 13 1993 4011 4022
7. D.J. Whyatt, E. de Boer, and F. Grosveld The two zinc finger-like domains of GATA-1 have different DNA binding specificities Eur. J. Biochem. 12 1993 4993 5005
8. H.-Y. Yang, and T. Evans Distinct roles for two cGATA-1 finger domains Mol. Cell. Biol. 12 1992 4562 4570
9. H.N. Arst Jr., and D.J. Cove Nitrogen metabolite repression in Aspergillus nidulans Mol. Gen. Genet. 126 1973 111 141
10. M. Stankovich, A. Platt, M.X. Caddick, T. Langdon, P.M. Shaffer, and H.N. Arst Jr. C-terminal truncation of the transcriptional activator encoded by areA in Aspergillus nidulans results in both loss-of-function and gain-of-function phenotypes Mol. Microbiol. 7 1993 81 87
11. T. Langdon, A. Sheerins, A. Ravagnani, M. Gielkins, M.X. Caddick, and H.N. Arst Jr. Mutational analysis reveals dispensability of the N-terminal region of the Aspergillus transcription factor mediating nitrogen metabolite repression Mol. Microbiol. 17 1995 877 888
12. M.R. Starich, M. Wikström, H.N. Arst Jr., G.M. Clore, and A.M. Gronenborn The solution structure of a fungal AreA protein-DNA complex: an alternative binding mode for the basic carboxyl tail of GATA factors J. Mol. Biol. 277 1998 605 620
13. A. Chant, X. Provatopoulou, I.W. Manfield, and G.G. Kneale Structural and functional characterisation of the DNA binding domain of the Aspergillus nidulans gene regulatory protein AreA Biochim. Biophys. Acta 1648 2003 84 89
14. I.W. Manfield, L.A. Reynolds, J. Gittins, and G.G. Kneale The DNA-binding domain of the gene regulatory protein AreA extends beyond the minimal zinc-finger region conserved between GATA proteins Biochim. Biophys. Acta 1493 2000 325 332
15. G. Mendoza-Hernandez, F. Minauro, and J.L. Rendon Aggregation, dissociation and unfolding of glucose dehydrogenase during urea denaturation Biochim. Biophys. Acta 1478 2000 221 231
16. G. Mendoza-Hernandez, and J.L. Rendon Human placental estradiol 17β-dehydrogenase: structural and catalytic changes during denaturation Biochim. Biophys. Acta 1297 1996 219 227
17. M. Yagi, K. Sakurai, C. Kalidas, C.A. Batt, and Y. Goto Reversible unfolding of bovine β-lactoglobulin mutants without a free thiol group J. Biol. Chem. 278 2003 47009 47015
18. M.L. Carpenter, and G.G. Kneale Analysis of DNA-protein interactions by intrinsic fluorescence Methods Mol. Biol. DNA-Protein Interact. 30 1994 313 325
19. M.L. Carpenter, and G.G. Kneale Circular dichroism and fluorescence analysis of the interaction of Pf1 gene 5 protein with poly dT J. Mol. Biol. 217 1991 681 689
20. D.B. Wetlaufer, J.T. Edsall, and B.R. Hollingworth Ultraviolet difference spectra of tyrosine groups in proteins and amino acids J. Biol. Chem. 233 1958 1421 1428
21. H.A. Sober, CRC Handbook of Biochemistry and Molecular Biology, Chemical Rubber Co., Cleveland
22. B. Birdsall, R.W. King, M.R. Wheeler, C.A. Lewis, S.R.B.D.R. Goode, and G.C.K. Roberts Correction for light absorption in fluorescence studies of protein-ligand interactions Anal. Biochem. 132 1983 353 361
23. R.C. Kelly, D.E. Jensen, and P.H. von Hippel DNA melting proteins. IV. Fluorescence measurements of binding parameters for bacteriophage T4 gene 32-protein to mono-, oligo-, and polynucleotides J. Biol. Chem. 251 1976 7240 7250
24. Y. Choo, A. Castellanos, B. Garcia-Hernandez, I. Sanchez-Garcia, and A. Klug Promoter-specific activation of gene expression directed by bacteriophage-selected zinc fingers J. Mol. Biol. 273 1997 525 532
25. H. Wu, W.P. Yang, and C.F.R. Barbas Building zinc fingers by selection: toward a therapeutic application Proc. Natl. Acad. Sci. USA 92 1995 344 348
26. A. Goel, D. Colcher, J.S. Koo, B.J. Booth, G. Pavlinkova, and S.K. Batra Relative position of the hexahistidine tag effects binding properties of a tumor-associated single-chain Fv construct Biochim. Biophys. Acta 1523 2000 13 20
27. I. Fonda, M. Keig, V. Gaberc-Porekar, P. Pristovaek, and V. Menart Attachment of histidine tags to recombinant tumor necrosis factor-alpha drastically changes its properties Sci. World J. 2 2002 1312 1325