Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide

Journal of Biotechnology

Volumn 145, Issue 2, 2010, Pages 130-138

  Gouveia, Ricardo ^a   Kandzia, Sebastian ^b   Conradt, Harald S ^b   Costa, Júlia ^a,c  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b GlycoThera GmbH   (Germany)

^c INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

Author keywords

Cell adhesion molecule L1; Dimethyl sulfoxide; Insect cells; N glycosylation; Stable expression system

Indexed keywords

CELL ADHESION MOLECULE L1; CELL ADHESION MOLECULES; INSECT CELLS; N-GLYCOSYLATION; STABLE EXPRESSION;

ADHESION; BIOMOLECULES; CELL ADHESION; CELL CULTURE; CELLS; CHROMATOGRAPHIC ANALYSIS; DESORPTION; ESTERIFICATION; GLYCOSYLATION; MASS SPECTROMETERS; MASS SPECTROMETRY; MOLECULES; NEGATIVE IONS; PULSED LASER APPLICATIONS; PULSED LASERS;

DIMETHYL SULFOXIDE;

BETA MANNOSE 4 N ACETYLGLUCOSAMINE; DIMETHYL SULFOXIDE; GLYCOPEPTIDASE; IMMUNOGLOBULIN; IMMUNOGLOBULIN DOMAIN 5; IMMUNOGLOBULIN DOMAIN 6; MANNOSE; N ACETYL BETA GLUCOSAMINIDASE; N ACETYLGLUCOSAMINE; NERVE CELL ADHESION MOLECULE L1; UNCLASSIFIED DRUG;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOTECHNOLOGICAL PRODUCTION; CARBOHYDRATE ANALYSIS; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; GENE EXPRESSION SYSTEM; GENETIC TRANSFECTION; INSECT CELL; LEPIDOPTERA; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; STABLE EXPRESSION;

ANIMALS; CELLS, CULTURED; CLONING, MOLECULAR; DIMETHYL SULFOXIDE; GENETIC ENHANCEMENT; GLYCOSYLATION; HUMANS; NEURAL CELL ADHESION MOLECULE L1; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SPODOPTERA; TRANSFECTION;

HEXAPODA; SPODOPTERA FRUGIPERDA; TRICHOPLUSIA NI;

EID: 73149095578     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.10.018     Document Type: Article

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