Gene transcription acceleration: Main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells

Biotechnology and Bioengineering

Volumn 97, Issue 3, 2007, Pages 526-535

  Wang, Wenying ^a   Yi, Xiaoping ^a   Zhang, Yuanxing ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Cell culture; CHO cell; Dimethyl sulfoxide (DMSO); Gene transcription; Hepatitis B surface antigen

Indexed keywords

ANTIGENS; BIOASSAY; CELL CULTURE; DIMETHYL SULFOXIDE; RNA;

CHO CELL; GENE TRANSCRIPTION; HEPATITIS B SURFACE ANTIGEN;

GENE TRANSFER;

DIMETHYL SULFOXIDE; HEPATITIS B SURFACE ANTIGEN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; GENETIC TRANSCRIPTION; HALF LIFE TIME; NONHUMAN; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; DIMETHYL SULFOXIDE; GENE EXPRESSION REGULATION, VIRAL; HEPATITIS B SURFACE ANTIGENS; HEPATITIS B VIRUS; TRANSCRIPTION, GENETIC;

CRICETULUS GRISEUS;

EID: 34249798439     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21262     Document Type: Article

References (25)

1. Alfonso J, Pollevick GD, Castensson A, Jazin E, Frasch ACC. 2002. Analysis of gene expression in the rat hippocampus using real time PCR reveals high inter-individual variation in mRNA expression levels. J Neurosci Res 67:225-234.
2. Argaw T, Ritzhaupt A, Wilson CA. 2002. Development of a real time quantitative PCR assay for detection of porcine endogenous retrovirus. J Viro Methods 106:97-106.
3. Berndt A, Heller M, Kosmehl H. 2002. Cytokine mRNA expression in experimental porcine pneumonia. Deut Tierarztl Wochenschr 109: 205-209.
4. Bustin SA. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-193.
5. Fiore M, Degrassi F. 1999. Dimethyl sulfoxide restores contact inhibition-induced growth arrest and inhibits cell density-dependent apoptosis in hamster cells. Exp Cell Res 251:102-110.
6. Fussenegger M, Schlatter S, Dätwyler D, Mazur X, Bailey JE. 1998. Controlled proliferation by mutigene metabolic engineering enhances the productivity of Chinese hamster ovary cells. Nat Biotechnol 16:468-472.
7. Heid CA, Stevens J, Livak KJ, Williams PM. 1996. Real-time quantitative PCR. Genome Res 6:986-994.
8. Huovila APJ, Eder AM, Fuller SD. 1992. Hepatitis B surface antigen assembles in a post-ER, pre-Golgi compartment. J Cell Biol 118: 1305-1320.
9. Juang JK, Liu HJ. 1987. The effect of DMSO on natural DNA conformation in enhancing transcription. Biochem Biophys Res Commun 146:1458-1464.
10. Kito M, Itami S, Fukano Y, Yamana K, Shibui T. 2002. Construction of engineered CHO strains for high-level production of recombinant proteins. Appl Microbiol Biotechnol 60:442-448.
11. Li JH, Sun XM, Zhang YX. 2006a. Improvement of hepatitis B surface antigen expression by dimethyl sulfoxide in the culture of recombinant Chinese hamster ovary cells. Process Biochem 41:317-322.
12. Li JH, Huang ZY, Sun XM, Yang PY, Zhang YX. 2006b. Understanding the enhanced effect of dimethyl sulfoxide on hepatitis B surface antigen expression in the culture of Chinese hamster ovary cells on the basis of proteome analysis. Enzyme Microb Technol 38:372-380.
13. Ling WLW, Deng L, Lepore J, Cutler C, Cannon-Carlson S, Wang Y, Voloch M. 2003. Improvement of monoclonal antibody production in hybridoma cells by dimethyl sulfoxide. Biotechnol Prog 19:158-162.
14. Liu CH, Chu IM, Hwang SM. 2001a. Pentanoic acid, a novel protein synthesis stimulant for Chinese hamster ovary (CHO) cells. J Biosci Bioeng 91:71-75.
15. Liu CH, Chu IM, Hwang SM. 2001b. Enhanced expression of various genes in recombinant Chinese hamster ovary cells in presence of dimethyl sulfoxide. Biotechnol Lett 23:1641-1645.
16. -ΔΔCt method. Methods 25:402-408.
17. Mackey IM, Arden KE, Nitsche A. 2002. Real-time PCR in virology. Nucleic Acids Res 30:1292-1305.
18. Pendse GJ, Karkare S, Bailey JE. 1992. Effect of cloned gene dosage on cell growth and hepatitis B surface antigen synthesis and secretion in recombinant CHO cells. Biotechnol Bioeng 40:119-129.
19. Ragione FD, Criniti V, Pietra VD, Borriello A, Oliva A, Indaco S, Yamamoto T, Zappia V. 2001. Genes modulated by histone acetylation as new effectors of butyrate activity. FEBS Lett 499:199-204.
20. Rolfe FG, Sewell WA. 1997. Analysis of human interleukin-5 gene transcription by a novel nuclear run on method based on the polymerase chain reaction. J Immunol Methods 202:143-151.
21. Santos NC, Coelho F, Silva JM, Saldanha C. 2003. Multidisciplinary utilization of dimethyl sulfoxide: Pharmacological, cellular, and molecular aspects. Biochem Pharmacol 65:1035-1041.
22. Stratling WH. 1976. Stimulation of transcription on chromatin by polar organic compounds. Nucleic Acids Res 3:1203-1213.
23. Teraoka H, Mikoshiba M, Takase K, Yamamoto K, Tsukada K. 1996. Reversible G1 arrest induced by dimethyl sulfoxide inhibits IL-6-induced differentiation of SKW6-CL4 into IgM-secreting plasma cells. Exp Cell Res 222:218-224.
24. Turakulov RI, Chistiakov DA. 2002. Comparison of DNA and RNA based real-time PCR assays for quantitative detection of chromosomal translocations. Biotechnol Lett 24:1709-1714.
25. Yoon SK, Song JY, Lee GM. 2003. Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnol Bioeng 82:289-298.