Expression of the human μ opioid receptor in a stable Sf9 cell line

Journal of Biotechnology

Volumn 95, Issue 2, 2002, Pages 181-187

  Kempf, Juliette ^a   Snook, Laelie A ^b   Vonesch, Jean Luc ^c   Dahms, Tanya E S ^b   Pattus, Franc ^a   Massotte, Dominique ^a,c  

^a UNIVERSITÉ DE STRASBOURG   (France)

^b UNIVERSITY OF REGINA   (Canada)

^c INSTITUT DE GÉNÉTIQUE ET DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

Author keywords

Baculovirus expression system; G protein coupled receptor; Human opioid receptor; Insect cell; OpIE2 promoter; Stable expression

Indexed keywords

BIOLOGICAL MEMBRANES; DNA; PROTEINS; VIRUSES;

IMMUNOFLUORESCENE IMAGES;

CELLS;

G PROTEIN COUPLED RECEPTOR; GLYCOPROTEIN; HISTIDINE; MU OPIATE RECEPTOR; SIGNAL PEPTIDE;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LINE; CELL MEMBRANE; CONTROLLED STUDY; GENETIC TRANSFECTION; HUMAN; IMMEDIATE EARLY GENE; IMMUNOFLUORESCENCE TEST; INSECT CELL; LEPIDOPTERA; MOLECULAR CLONING; NONHUMAN; POLYHEDROSIS VIRUS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; TRANSCRIPTION REGULATION;

ANIMALS; BACULOVIRIDAE; BIOTECHNOLOGY; CELL LINE; GENE EXPRESSION REGULATION; GTP-BINDING PROTEINS; HUMANS; PROMOTER REGIONS (GENETICS); RECEPTORS, OPIOID, MU; SPODOPTERA; TRANSFECTION;

INSECTA; ORGYIA; ORGYIA PSEUDOTSUGATA; SPODOPTERA; SPODOPTERA FRUGIPERDA; UNIDENTIFIED BACULOVIRUS;

EID: 0037045856     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(02)00008-1     Document Type: Article

References (20)

1. Efficient membrane targeting of the chick nicotinic acetylcholine receptor α-subunit in stable insect cell lines
2. A single nucleotide polymorphic mutation in the human μ-opioid receptor severely impairs receptor signaling
3. Overexpression of integral membrane proteins for structural studies
4. Engineering of a proteolytically stable human beta 2-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells
5. A series of broad host range shuttle vectors for constitutive and inducible expression of heterologous proteins in insect cell lines
6. Recent advances in molecular recognition and signal transduction of active peptides: Receptors for opioid peptides
7. Human β2-adrenergic receptor produced in stably transformed insect cells is functionally coupled via endogenous GTP-binding protein to adenylyl-cyclase
8. Molecular cloning and pharmacology of cloned opioid receptors
9. Production of functional antibodies generated in a nonlytic insect cell expression system
10. Recovery of homogeneous and functional beta 2-adrenergic receptors from extracellular baculovirus particles
11. Characterization of δ, κ and μ human opioid receptors overexpressed in baculovirus-infected insect cells
12. A molecular basis for opioid action
13. Parameters influencing human mu opioid receptor over-expression in baculovirus-infected insect cells
14. Stable insect cell cultures for recombinant protein production
15. Opioids, opioid receptors, and the immune response
16. The human mu opioid receptor: Modulation of functional desensitization by calcium/calmodulin-dependent protein kinase and protein kinase C
17. Baculovirus immediate-early promoter-mediated expression of the Zeocin™ resistance gene for use as a dominant selectable marker in Dipteran and Lepidopteran insect cell lines
18. Heterologous expression of G-protein-coupled receptors: Human opioid receptors under scrutiny
19. Expression and pharmacological characterization of the human μ-opioid receptor in the methylotrophic yeast Pichia pastoris
20. Endogenous opiates: 1999