False positive reactivity of recombinant, diagnostic, glycoproteins produced in High Five™ insect cells: Effect of glycosylation

Journal of Immunological Methods

Volumn 330, Issue 1-2, 2008, Pages 130-136

  Hancock, Kathy ^a   Narang, Someet ^b   Pattabhi, Sowmya ^a   Yushak, Melinda L ^a   Khan, Azra ^a   Lin, Seh ching ^a   Plemons, Robert ^b   Betenbaugh, Michael J ^b   Tsang, Victor C W ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

Cysticercosis; Diagnostic antigens; High Five; Insect cell glycosylation; Recombinant proteins; Spodoptera frugiperda (Sf9); Trichoplusia ni

Indexed keywords

GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORED PROTEIN; PROTEIN GP50; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYSTICERCOSIS; FALSE NEGATIVE RESULT; GLYCOSYLATION; INSECT CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; TAENIA SOLIUM;

ANIMALS; ANTIBODIES, HELMINTH; ANTIGENS, HELMINTH; BACULOVIRIDAE; CELL LINE; CYSTICERCOSIS; FALSE POSITIVE REACTIONS; GENETIC VECTORS; GLYCOPROTEINS; GLYCOSYLATION; HELMINTH PROTEINS; HUMANS; PREDICTIVE VALUE OF TESTS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SPODOPTERA; TAENIA SOLIUM;

HEXAPODA; SPODOPTERA FRUGIPERDA; TAENIA SOLIUM; TRICHOPLUSIA NI;

EID: 38349134874     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.08.002     Document Type: Article

References (23)

1. Ailor E., Takahashi N., Tsukamoto Y., Masuda K., Rahman B.A., Jarvis D.L., Lee Y.C., and Betenbaugh M.J. N-glycan patterns of human transferrin produced in Trichoplusia ni insect cells: effects of mammalian galactosyltransferase. Glycobiology 10 (2000) 837
2. Altmann F., Staudacher E., Wilson I.B., and Marz L. Insect cells as hosts for the expression of recombinant glycoproteins. Glycoconj. J. 16 (1999) 109
3. Fabini G., Freilinger A., Altmann F., and Wilson I.B. Identification of core alpha 1,3-fucosylated glycans and cloning of the requisite fucosyltransferase cDNA from Drosophila melanogaster. Potential basis of the neural anti-horseradish peroxidase epitope. J. Biol. Chem. 276 (2001) 28058
4. Faveeuw C., Mallevaey T., Paschinger K., Wilson I.B., Fontaine J., Mollicone R., Oriol R., Altmann F., Lerouge P., Capron M., and Trottein F. Schistosome N-glycans containing core alpha 3-fucose and core beta 2-xylose epitopes are strong inducers of Th2 responses in mice. Eur. J. Immunol. 33 (2003) 1271
5. Garcia H.H., Gonzalez A.E., Evans C.A., and Gilman R.H. Taenia solium cysticercosis. Lancet 362 (2003) 547
6. Gemmell M., Matyas Z., Pawlowski Z., and Soulsby E.J.L. Guidelines for Surveillance Prevention and Control of Taeniasis/Cysticercosis (1983), WHO 69
7. Hancock K., Pattabhi S., Greene R.M., Yushak M.L., Williams F., Khan A., Priest J.W., Levine M.Z., and Tsang V.C. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol. Biochem. Parasitol. 133 (2004) 115 [erratum appears in Mol Biochem Parasitol. 2004 Apr;134(2):285]
8. Hancock K., Pattabhi S., Whitfield F.W., Yushak M.L., Lane W.S., Garcia H.H., Gonzalez A.E., Gilman R.H., and Tsang V.C. Characterization and cloning of T24, a Taenia solium antigen diagnostic for cysticercosis. Mol. Biochem. Parasitol. 147 (2006) 109
9. Hotez P.J., Bundy D.A.P., Beegle K., Brooker S., Drake L., de Silva N., Montresor A., Engels D., Jukes M., Chitsulo L., Chow J., Laxminarayan R., Michaud C.M., Bethony J., Correa-Oliveira R., Shu-Hua X., Fenwick A., and Savioli L. Helminth infections: soil-transmitted helminth infections and schistosomiasis. In: Jamison D.T., Breman J.G., Measham A.R., Alleyne G., Claeson M., Evans D.B., Jha P., Mills A., and Musgrove P. (Eds). Disease Control Priorities in Developing Countries. 2nd ed. (2006), Oxford University Press, New York 467
10. Hsu T.A., Takahashi N., Tsukamoto Y., Kato K., Shimada I., Masuda K., Whiteley E.M., Fan J.Q., Lee Y.C., and Betenbaugh M.J. Differential N-glycan patterns of secreted and intracellular IgG produced in Trichoplusia ni cells. J. Biol. Chem. 272 (1997) 9062
11. Kim Y.K., Shin H.S., Tomiya N., Lee Y.C., Betenbaugh M.J., and Cha H.J. Production and N-glycan analysis of secreted human erythropoietin glycoprotein in stably transfected Drosophila S2 cells. Biotechnol. Bioeng. 92 (2005) 452
12. Levine M.Z., Calderon J.C., Wilkins P.P., Lane W.S., Asara J.M., Hancock K., Gonzalez A.E., Garcia H.H., Gilman R.H., and Tsang V.C. Characterization, cloning, and expression of two diagnostic antigens for Taenia solium tapeworm infection. J. Parasitol. 90 (2004) 631
13. Pan American Health Organization. PAHO/WHO Informal Consultation on the Taeniosis/Cysticercosis Complex (1997), Pan American Health Organization, Brasilia, Brazil
14. Rendic D., Linder A., Paschinger K., Borth N., Wilson I.B., and Fabini G. Modulation of neural carbohydrate epitope expression in Drosophila melanogaster cells. J. Biol. Chem. 281 (2006) 3343
15. Staudacher E., Kubelka V., and Marz L. Distinct N-glycan fucosylation potentials of three lepidopteran cell lines. Eur. J. Biochem. 207 (1992) 987
16. Staudacher E., Altmann F., Wilson I.B., and Marz L. Fucose in N-glycans: from plant to man. Biochim. Biophys. Acta 1473 (1999) 216
17. Tomiya N., Howe D., Aumiller J.J., Pathak M., Park J., Palter K.B., Jarvis D.L., Betenbaugh M.J., and Lee Y.C. Complex-type biantennary N-glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [beta]-1,4-galactosyltransferase and [beta]-1,2-N-acetylglucosaminyltransferase II. Glycobiology 13 (2003) 23
18. Tomiya N., Narang S., Lee Y.C., and Betenbaugh M.J. Comparing N-glycan processing in mammalian cell lines to native and engineered lepidopteran insect cell lines. Glycoconj. J. 21 (2004) 343
19. Tsang V.C.W., Peralta J.M., and Simons A.R. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 92 (1983) 377
20. Tsang V.C., Brand J.A., and Boyer A.E. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J. Infect. Dis. 159 (1989) 50
21. 4) of horseradish peroxidase to antibodies for use in enzyme-linked immunosorbent assay. J. Immunoass. 16 (1995) 395
22. van Die I., Gomord V., Kooyman F.N., van den Berg T.K., Cummings R.D., and Vervelde L. Core alpha1->3-fucose is a common modification of N-glycans in parasitic helminths and constitutes an important epitope for IgE from Haemonchus contortus infected sheep. FEBS Lett. 463 (1999) 189
23. Voss T., Ergulen E., Ahorn H., Kubelka V., Sugiyama K., Maurer-Fogy I., and Glossl J. Expression of human interferon omega 1 in Sf9 cells. No evidence for complex-type N-linked glycosylation or sialylation. Eur. J. Biochem. 217 (1993) 913