Improved production of recombinant tumstatin in stably transformed Trichoplusia ni BTI Tn 5B1-4 cells

Protein Expression and Purification

Volumn 35, Issue 1, 2004, Pages 69-75

  Chang, Kyung Hwa ^a   Lee, Jong Min ^a   Jeon, Hee Kyoung ^a   Chung, In Sik ^a  

^a Kyung Hee University   (South Korea)

Author keywords

Activity; Dimethyl sulfoxide; Expression; Recombinant tumstatin; Sodium butyrate; Tn 5b1 4 cells

Indexed keywords

TRICHOPLUSIA NI;

EID: 16544364788     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.01.004     Document Type: Article

References (18)

1. D. Hanahan, J. Folkman, Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis, Cell 86 (1996) 353-364.
2. M.S. O'Reilly, T. Boehm, Y. Shing, N. Fukai, G. Vasios, W.S. Lane, E. Flynn, J.R. Birkhead, B.R. Olsen, J. Folkman, Endostatin: an endogenous inhibitor of angiogenesis and tumor growth, Cell 88 (1997) 277-285.
3. Y. Maeshima, P.C. Colorado, A. Torre, K.A. Holthaus, J.A. Grunkemeyer, M.B. Ericksen, H. Hopfer, Y. Xiao, I.E. Stillman, R. Kalluri, Distinct antitumor properties of a type IV collagen domain derived from basement membrane, J. Biol. Chem. 275 (2000) 21340-21348.
4. D.C. Darland, P.A. D'Amore, Blood vessel maturation: vascular development comes of age, J. Clin. Invest. 103 (1999) 157-158.
5. Y. Maeshima, M. Manfredi, C. Reimer, K.A. Holthaus, H. Hopfer, B.R. Chandamuri, S. Kharbanda, R. Kalluri, Identification of the anti-angiogenic site within vascular basement membrane-derived tumstatin, J. Biol. Chem. 276 (2001) 15240-15248.
6. Y. Maeshima, A. Sudhakar, J.C. Lively, K. Ueki, S. Kharbanda, R. Kahn, N. Sonenberg, R.O. Hynes, R. Kalluri, Tumstatin, an endothelial cell-specific inhibitor of protein synthesis, Science 295 (2002) 140-143.
7. H.K. Jeon, K.H. Chang, K.I. Kim, I.S. Chung, Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells, Biotechnol. Lett. 25 (2003) 185-189.
8. D.J. Finney, Probit Analysis, third ed., Cambridge University Press, Cambridge.
9. U.K. Laemmli, Cleavage of structural proteins during the assembly of head of bacteriophage, Nature 227 (1970) 680-685.
10. S. Kawai, M. Nishizawa, New procedure for DNA transfection with polycation and dimethyl sulfoxide, Mol. Cell. Biol. 4 (1984) 1172-1174.
11. H. Melkonyan, C. Sorg, M. Klempt, Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO), Nucleic Acids Res. 24 (1996) 4356-4357.
12. M.F. Wahl, G.H. An, J.M. Lee, Effects of dimethylsulfoxide on heavy chain monoclonal antibody production from plant cell culture, Biotechnol. Lett. 17 (1995) 463-468.
13. T.R. Henderson, R.F. Henderson, J.L. York, Effect of dimethyl sulfoxide on subunit proteins, Ann. N.Y. Acad. Sci. 243 (1975) 38-53.
14. H. Saito, T. Kagawa, S. Tada, S. Tsunematsu, F.M. Guevara, T. Watanabe, T. Morizane, M. Tsuchiya, Effect of dexamethasone, dimethylsulfoxide and sodium butyrate on a human hepatoma cell line PLC/PRF/5, Cancer Biochem. Biophys. 13 (1992) 75-84.
15. S.P. Tam, X. Zhang, C. Cuthbert, Z. Wang, T. Ellis, Effects of dimethylsulfoxide on apolipoprotein A-1 in the human hepatoma cell line, J. Lipid Res. 38 (1997) 2090-2102.
16. K.H. Chang, J.H. Park, Y.H. Lee, J.H. Kim, H.O. Chun, J.H. Kim, I.S. Chung, Dimethylsulfoxide and sodium butyrate enhance the production of recombinant cyclooxygenase 2 in stably transformed Drosophila melanogaster S2 cells, Biotechnol. Lett. 24 (2002) 1353-1359.
17. D.P. Palermo, M.E. DeGraaf, K.R. Marotti, E. Rehberg, L.E. Post, Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression, J. Biotechnol. 19 (1991) 35-48.
18. S.K.W. Oh, P. Vig, F. Chua, W.K. Teo, M.G.S. Yap, Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate, Biotechnol. Bioeng. 42 (1993) 601-610.