Comparison of the N-linked glycosylation of human β1,3-N-acetylglucosaminyltransferase 2 expressed in insect cells and silkworm larvae

Journal of Biotechnology

Volumn 143, Issue 1, 2009, Pages 27-33

  Dojima, Takashi ^a   Nishina, Takuya ^a   Kato, Tatsuya ^a   Uno, Tsuyoshi ^b   Yagi, Hirokazu ^b   Kato, Koichi ^b,c,d,e   Park, Enoch Y ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

^b NAGOYA CITY UNIVERSITY   (Japan)

^c INSTITUTE FOR MOLECULAR SCIENCE   (Japan)

^d GLYENCE Co Ltd   (Japan)

^e OCHANOMIZU UNIVERSITY   (Japan)

Author keywords

1,3N Acetylglucosaminyltransferase 2; BmNPV bacmid; Insect cell; N Glycosylation; Silkworm larvae

Indexed keywords

2-AMINOPYRIDINE; BIOLOGICAL FUNCTIONS; BMNPV BACMID; EXPRESSION SYSTEM; INSECT CELL; INSECT CELLS; LEPIDOPTERAN INSECTS; N-GLYCAN; N-GLYCAN STRUCTURES; N-GLYCOSYLATION; N-LINKED GLYCOSYLATION; SILKWORM LARVAE; TERMINAL RESIDUES; TRICHOPLUSIA NI;

CELL MEMBRANES; ESTERIFICATION; LABELS;

GLYCOSYLATION;

2 AMINOPYRIDINE; BETA 1,3 N ACETYLGLUCOSAMINYLTRANSFERASE; FUCOSE; GLYCAN; N ACETYLGLUCOSAMINYLTRANSFERASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; INSECT CELL; LARVA; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SILKWORM;

AMINOPYRIDINES; ANIMALS; BOMBYX; CARBOHYDRATE SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLYCOSYLATION; GREEN FLUORESCENT PROTEINS; HEMOLYMPH; HUMANS; INSECTS; LECTINS; MOLECULAR SEQUENCE DATA; N-ACETYLGLUCOSAMINYLTRANSFERASES; POLYSACCHARIDES; PROTEIN STRUCTURE, TERTIARY;

BOMBYX MORI; BOMBYX MORI NPV; HEXAPODA; TRICHOPLUSIA NI;

EID: 67651001558     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.06.013     Document Type: Article

References (32)

1. Altmann F., Schwihla H., Staudacher E., Glössl J., and März L. Insect cells contain an unusual, membrane-bound β-N-acetylglucosaminidase probably involved in the processing of protein N-glycans. J. Biol. Chem. 270 (1995) 17344-17349
2. Altmann F., Staudacher E., Wilson I.B.H., and Marz L. Insect cells as hosts for the expression of recombinant glycoproteins. Glycoconj. J. 16 (1999) 109-123
3. Chui D., Oh-Edo M., Liao Y.F., Panneerselvam K., Lal A., Marek K.W., Freeze H.H., Moremen K.W., Fukuda M.N., and Marth J.D. α-Mannosidase-II deficiency results in dyserythropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis. Cell 90 (1997) 157-167
4. Dojima T., Nishina T., Kato T., Ueda H., and Park E.Y. Comparative study of affinity-tagged proteins for efficient purification of recombinant secretory protein expressed in silkworm larval hemolymph. Biotechnol. Bioprocess. Eng. 14 (2009) 281-287
5. Hogeland K.E., and Deinzer M.L. Mass spectrometric studies on the N-linked oligosaccharides of baculovirus-expressed mouse interleukin-3. Biol. Mass Spectrom. 23 (1994) 218-224
6. Hsu T.A., Takahashi Y., Tsukamoto Y., Kato K., Shimada I., Masuda K., Whiteley E.M., Finn J.Q., Lee Y.C., and Betengaugh M.J. Differential N-glycan patterns of secreted and intracellular IgG produced in Trichoplusia ni cells. J. Biol. Chem. 272 (1997) 9062-9070
7. Jenkins N., and Curling E.M. Glycosylation of recombinant proteins: problems and prospects. Enzyme Microb. Technol. 16 (1994) 354-364
8. Jenkins N., Parekh R.B., and James D.C. Getting the glycosylation right: implications for the biotechnology industry. Nat. Biotechnol. 14 (1996) 975-981
9. Joshi L., Davis T.R., Mattu T.S., Rudd P.M., Dwek R.A., Shuler M.L., and Wood H.A. Influence of baculovirus-host cell interactions on complex N-linked glycosylation of a recombinant human protein. Biotechnol. Prog. 16 (2000) 650-656
10. uv-β3GnT2 fusion protein production by suppressing protease in baculovirus expression system. Biosci. Biotechnol. Biochem. 67 (2003) 2388-2395
11. uv-β1,3-N-acetylglucosaminyltransferase 2 production in two insect-cell-based expression systems. Protein Expr. Purif. 35 (2004) 54-61
12. uv-β1,3-N-acetylglucosaminyltransferase 2 fusion protein using a molecular chaperone-assisted insect-cell-based expression system. Biotechnol. Bioeng. 89 (2005) 424-433
13. Kato T., Suzuki M., Murata T., and Park E.Y. The effects of N-glycosylation sites and the N-terminal region on the biological function of β1,3-N-acetylglucosaminyltransferase 2 and its secretion. Biochem. Biophys. Res. Commun. 329 (2005) 699-705
14. Kawar Z., Karaveg K., Moremen K.W., and Jarvis D.L. Insect cells encode a class II α-mannosidase with unique properties. J. Biol. Chem. 276 (2001) 16335-16340
15. Kulakosky P.C., Hughes P.R., and Wood H.A. N-linked glycosylation of a baculovirus-expressed recombinant glycoprotein in insect larvae and tissue culture cells. Glycobiology 8 (1998) 741-745
16. Murata T., Inukai T., Suzuki M., Yamagishi M., and Usui T. Facile enzymatic conversion of lactose into lacto-N-tetraose and lacto-N-neotetrose. Glycoconj. J. 16 (1995) 189-195
17. Murata T., and Usui T. Enzymatic synthesis of oligosaccharides and neoglycoconjugates. Biosci. Biotechnol. Biochem. 70 (2006) 1049-1059
18. Nakagawa H., Kawamura Y., Kato K., Shimada I., Arata Y., and Takahashi N. Identification of neutral and sialyl N-linked oligosaccharide structures from human serum glycoproteins using three kinds of high-performance liquid chromatography. Anal. Biochem. 226 (1995) 130-138
19. Oganah O.W., Freedman R.B., Jenkins N., Patel K., and Rooney B.C. Isolation and characterization of an insect cell line able to perform complex N-linked glycosylation on recombinant proteins. Biotechnology 14 (1996) 197-202
20. Park E.Y., Kageshima A., Kwon M.S., and Kato T. Enhancement production of secretory β1,3N-acetylglucosaminyltransferase 2 fusion protein into hemolymph of Bombyx mori larvae using recombinant BmNPV bacmid integrated signal sequence. J. Biotechnol. 129 (2007) 681-688
21. Park E.Y., Ishikiriyama M., Nishina T., Kato T., Yagi H., Kato K., and Ueda H. Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and N-linked glycan structure. J. Biotechnol. 139 (2009) 108-114
22. Pristal J.L., Sarkar M., Schacher H., and Marth J.D. Isolation, characterization and inactivation of the mouse Mgat3 gene: the bisecting N-acetylglucosamine in asparagine-linked oligosaccharides appears dispensable for viability and reproduction. Glycobiology 7 (1997) 45-56
23. Rouslahti E. Proteoglycans in cell regulation. J. Biol. Chem. 264 (1989) 13369-13372
24. Shinkawa T., Nakamura K., Yamane N., Saijo-Hosaka E., Kanda Y., Sakurada M., Uchida K., Anazawa H., Satoh M., Yamasaki M., Hanai N., and Kenya S. The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type antibody-dependent cellular cytotoxicity. J. Biol. Chem. 31 (2003) 3466-3473
25. Shiraishi N., Natsume A., Togayachi A., Endo T., Akashima T., Yamada Y., Imai N., Nakagawa S., Koizumi K., Sekine S., Narimatsu H., and Sasaki K. Identification and characterization of three novel β1,3-N-acetylglucosaminyltransferases structurally related to the β1,3-galactosyltransferase family. J. Biol. Chem. 276 (2001) 3498-3507
26. Staudacher E., and Marz L. Strict order of (Fuc to Asn-linked GlcNAc) fucosyltransferases forming core-difucosylated structure. Glycoconj. J. 15 (1998) 355-360
27. Takahashi N., Nakagawa H., Fujikawa K., Kawamura Y., and Tomiya N. Three-dimensional elution mapping of pyridylaminated N-linked neutral and sialyl oligosaccharides. Anal. Biochem. 226 (1995) 139-146
28. Takahashi N., and Kato K. GALAXY (glycoanalysis by the three axes of MS and chromatography):a web application that assists structural analysis of N-glycans. Trends Glycosci. Glycotechnol. 15 (2003) 235-251
29. Tomiya N., Kurono M., Ishihara H., Tejima S., Endo S., Arata Y., and Takahashi N. Structural analysis of N-linked oligosaccharides by a combination of glycopeptidase, exoglycosidases, and high-performance liquid chromatography. Anal. Biochem. 163 (1987) 489-499
30. Varki A. Biological roles of oligosaccharides: all of the theories are correct. Glycobiology 3 (1993) 97-130
31. Werner R.G., Kopp K., and Schlueter M. Glycosylation of therapeutic proteins in different production systems. Acta Paediatr. Suppl. 96 (2007) 17-22
32. Yagi H., Takahashi N., Yamaguchi Y., Kimura N., Uchimura K., Kannagi R., and Kato K. Development of structural analysis of sulfated N-glycans by multidimensional high performance liquid chromatography mapping methods. Glycobiology 15 (2005) 1051-1060