Stable expression of recombinant human α3/4 fucosyltransferase III in Spodoptera frugiperda Sf9 cells

Journal of Biotechnology

Volumn 106, Issue 1, 2003, Pages 69-75

  Morais, V A ^a,b   Costa, J ^a,b  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

Author keywords

Human fucosyltransferase III; Insect cell; Stable expression

Indexed keywords

CELL CULTURE; CELLS; CLONING; SYNTHESIS (CHEMICAL); TISSUE;

TRANSCRIPTIONAL CONTROL;

BIOTECHNOLOGY;

BACULOVIRUS VECTOR; BLASTICIDIN S; FUCOSYLTRANSFERASE; GLYCOCONJUGATE; RECOMBINANT ENZYME; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CELL CULTURE; CONTROLLED STUDY; EXPRESSION VECTOR; GENE EXPRESSION; INSECT CELL; KIDNEY; LEPIDOPTERA; MILK; MOLECULAR CLONING; NONHUMAN; POLYHEDROSIS VIRUS; PRIORITY JOURNAL; PROMOTER REGION; STOMACH MUCOSA; WESTERN BLOTTING;

INSECTA; ORGYIA; ORGYIA PSEUDOTSUGATA; SPODOPTERA; SPODOPTERA FRUGIPERDA; UNIDENTIFIED BACULOVIRUS; VECTORS;

EID: 0242494111     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2003.08.002     Document Type: Article

References (19)

1. Ailor E., Betenbaugh M.J. Overexpression of a cytosolic chaperone to improve solubility and secretion of a recombinant IgG protein in insect cells. Biotechnol. Bioeng. 58:1998;196-203.
2. Ailor E., Betenbaugh M.J. Modifying secretion and post-translational processing in insect cells. Cur. Opin. Biotech. 10:1999;142-145.
3. Altmann F., Staudacher E., Wilson I.B.H., März L. Insect cells as hosts for the expression of recombinant glycoproteins. Glycoconj. J. 16:1999;109-123.
4. Britten C.J., van den Eijnden D.H., McDowell W., Kelly V.A., Witham S.J., Edbrooke M.R., Bird M.I., de Vries T., Smithers N. Acceptor specificity of the human leukocyte alpha3 fucosyltransferase: role of FucT-VII in the generation of selectin ligands. Glycobiology. 8:1998;321-327.
5. Costa J., Grabenhorst E., Nimtz M., Conradt H.S. Stable expression of the Golgi form and secretory variants of human fucosyltransferase III from BHK-21 cells. J. Biol. Chem. 272:1997;11613-11621.
6. Grabenhorst E., Hoffmann A., Nimtz M., Zettlmeissl G., Conradt H.S. Construction of stable BHK-21 cells coexpressing human secretory glycoproteins and human Gal (β1-4) GlcNAc-R α2,6-sialyltransferase. α2,6-Linked NeuAc is preferentially attached to the Gal (β1-4)GlcNAc(b1-2)Man(α1-3)-branch of diantennary oligosaccharides from secreted recombinant β-trace protein. Eur. J. Biochem. 232:1995;718-725.
7. Hollister J.R., Shaper J.H., Jarvis D.L. Stable expression of mammalian beta 1,4-galactosyltransferase extends the N-glycosylation pathway in insect cells. Glycobiology. 8:1998;473-480.
8. Johnson P.H., Donald A.S.R., Watkins W.M. Purification and properties of the a-3/4-L-fucosyltransferase released into the culture medium during the growth of the human A431 epidermoid carcinoma cell line. Glycoconj. J. 10:1993;152-164.
9. Kempf J., Snook L.A., Vonesch J.L., Dahms T.E.S., Pattus F., Massotte D. Expression of the human μ opioid receptor in a stable Sf9 cell line. J. Biotechnol. 95:2002;181-187.
10. Kukowska-Latallo J.F., Larsen R.D., Nair R.P., Lowe J.B. A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group α(1,3/1,4)fucosyltransferase. Genes Dev. 4:1990;1288-1303.
11. Li E., Brown S.L., Dolman C.S., Brown G.B., Nemerow G.R. Production of functional antibodies generated in a nonlytic insect cell expression system. Protein Exp. Purif. 21:2001;121-128.
12. Lowe, J.B., 1994. Carbohydrate recognition in cell-cell interaction. In: Fukuda, M., Hindsgaul, O. (Eds.), Molecular Glycobiology. Oxford University Press, New York, pp. 163-205.
13. McCarroll L., King L.A. Stable insect cell cultures for recombinant protein production. Curr. Opin. Biotech. 8:1997;590-594.
14. Morais V.A., Serpa J., Palma A.S., Costa T., Maranga L., Costa J. Expression and characterization of recombinant human alpha-3/4- fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system. Biochem. J. 353:2001;719-725.
15. Morais V.A., Costa M.T., Costa J. N-Glycosylation of recombinant human fucosyltransferase III is required for its in vivo folding in mammalian and insect cells. Biochim. Biophys. Acta. 1619:2003;133-138.
16. Pfeifer T.A., Hegedus D.D., Grigliatti T.A., Theilmann D.A. Baculovirus immediate-early promoter-mediated expression of the Zeocin resistance gene for use as a dominant selectable marker in dipteran and lepidopteran insect cell lines. Gene. 188:1997;183-190.
17. Sasai K., Ikeda Y., Fujii T., Tsuda T., Taniguchi N. UDP-GlcNAc concentration is an important factor in the biosynthesis of beta1,6-branched oligosaccharides: regulation based on the kinetic properties of N-acetylglucosaminyltransferase V. Glycobiology. 12:2002;119-127.
18. Toki D., Sarkar M., Yip B., Reck F., Joziasse D., Fukuda M., Schachter H., Brockhausen I. Expression of stable human O-glycan core 2 β-1,6-N-acetylglucosaminyltransferase in Sf9 insect cells. Biochem. J. 325:1997;63-69.
19. Watkins, W.M., 1995. Molecular basis of antigenic specificity in the ABO, H and Lewis blood-group systems. In: Montreuil, J., Vliegenthart, J.F.G., Schachter, H. (Eds.), Glycoproteins, vol. 29a. Elsevier, Amsterdam, pp. 313-390.