High-level expression of secreted glycoproteins in transformed lepidopteran insect cells using a novel expression vector

Biotechnology and Bioengineering

Volumn 60, Issue 6, 1998, Pages 656-663

  Farrell, Patrick J ^a   Lu, Maolong ^a,c   Prevost, Jay ^b   Brown, Chris ^b   Behie, Leo ^a   Iatrou, Kostas ^a  

^a UNIVERSITY OF CALGARY   (Canada)

^b UNIVERSITY OF CALGARY   (Canada)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Baculovirus enhancer; Baculovirus transcriptional activator; Cell transformation; Insect cells; Lepidoptera; Protein expression; Secreted glycoproteins; Silkmoth actin gene promoter

Indexed keywords

ANTIBIOTICS; BATCH CELL CULTURE; CLONING; GENE TRANSFER; PROTEINS; VIRUSES;

BACULOVIRUS ENHANCER; BACULOVIRUS TRANSCRIPTIONAL ACTIVATOR; CELL TRANSFORMATION; LEPIDOPTERA; LEPIDOPTERAN INSECT CELLS; PROTEIN EXPRESSION; SECRETED GLYCOPROTEINS; SILKMOTH ACTIN GENE PROMOTER;

GROWTH KINETICS;

ACTIN; GLYCOPROTEIN; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR RECEPTOR; GREEN FLUORESCENT PROTEIN; JUVENILE HORMONE; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL TRANSFORMATION; CONTROLLED STUDY; ENHANCER REGION; EXPRESSION VECTOR; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LEPIDOPTERA; MAMMAL CELL; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SECRETION;

ANIMALS; CARBOXYLIC ESTER HYDROLASES; CELL TRANSFORMATION, VIRAL; CELLS, CULTURED; CRICETINAE; CYTOMEGALOVIRUS; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENETIC VECTORS; GLYCOPROTEINS; HUMANS; LEPIDOPTERA; NUCLEOPOLYHEDROVIRUS; RECEPTORS, GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; RECOMBINANT PROTEINS; SPODOPTERA;

ANIMALIA; BOMBYX MORI NUCLEOPOLYHEDROVIRUS; HEXAPODA; HUMAN HERPESVIRUS 5; INSECTA; LEPIDOPTERA; MAMMALIA; UNIDENTIFIED BACULOVIRUS;

EID: 0032553865     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19981220)60:6<656::AID-BIT2>3.0.CO;2-9     Document Type: Article

References (34)

1. Bonning, B. C., Hammock, B. D. 1995. Use of juvenile hormone esterase as a novel reporter enzyme in the baculovirus expression system. J. Vir. Meth. 51: 103-114.
2. Bonning, B. C., Ward, V. K., van Meer, M. M. M., Booth, T. F., Hammock, B. D. 1997. Disruption of lysosomal targeting is associated with insecticidal potency of juvenile hormone esterase. Proc. Natl. Acad. Sci. 94: 6007-6012.
3. Brown, C. B., Beaudry, P., Laing, T. D., Shoemaker, S., Kaushansky, K. 1995. In vitro characterization of the human recombinant soluble granulocyte-macrophage colony stimulating factor receptor. Blood 85: 1488-1495.
4. Copeland, W. C., Wang, T.-S.-F. 1991. Catalytic subunit of human DNA polymerase a overproduced from baculovirus-infected insect cells. J. Biol. Chem. 25: 2739-22748.
5. Davis, T. R., Wood, H. A. 1995. Intrinsic glycosylation potentials of insect cell cultures and insect larvae. In Vitro Cell. Dev. Biol. 31: 659-663.
6. Dee, K. U., Shuler, M. L., Wood, H. A. 1997. Inducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhanced recombinant protein production. Biotechnol. Bioeng. 54: 191-205.
7. Gearing, D. P., King, J. A., Gough, N. M., Nicola, N. A. 1989. Expression cloning of a receptor for human-granulocyte-macrophage colony-stimulating factor. EMBO J 8: 3667-3676.
8. Giordano, T. J., McAllister, W. T. 1990. Optimization of the hygromycin B resistance conferring gene as a dominant selectable marker in mammalian cells. Gene 88: 285-288.
9. Graham, F. L., Van der Eb, A. J. 1973. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52: 456.
10. Grossmann, M., Wong, R., The, N. G., Tropea, J. E., East-Palmer, J., Weintraub, B. D., Szkudlinski, M. W. 1997. Expression of biologically active human thyrotropin (hTSH) in a baculovirus system: Effect of insect cell glycosylation on hTSH activity in vitro and in vivo. Endocrinology 138: 92-100.
11. Ha, D. S., Schwarz, J. K., Turco, S. J., Beverley, S. M. 1996. Use of the green fluorescent protein as a marker in transfected Leishmania. Mol. Biochem. Parasitol. 77: 57-64.
12. Hanzlik, T. N., Abdel-Aal, Y. A. I., Harshman, L. G., Hammock, B. D. 1989. Isolation and sequencing of cDNA clones coding for juvenile hormone esterase from Heliothis virescens: Evidence for a charge relay network of serine esterases different from the serine proteases. J. Biol. Chem. 264: 12419-12425.
13. Hill-Perkins, M. S., Possee, R. D. 1990. A baculovirus expression vector derived from the basic protein promoter of Autographa californica nuclear polyhedrosis virus. J. Gen. Virol. 71: 971-976.
14. Jarvis, D. L., Fleming, J.-A. G. W., Kovacs, G. R., Summers, M. D., Guarino, L. A. 1990. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Bio/Technology 8: 950-955.
15. Jarvis, D. L., Summers, M. D. 1989. Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected cells. Mol. Cell. Biol. 9: 214-223.
16. Jarvis, D. L., Weinkauf, C., Guarino, L. A. 1996. Immediate-early baculovirus vectors for foreign gene expression in transformed or infected insect cells. Prot. Exp. Purif. 8: 191-203.
17. A receptors in stable insect cell lines. FEBS Lett. 335: 61-64.
18. Johnson, R., Meidinger, R. G., Iatrou, K. 1992. A cellular promoter-based expression cassette for generating recombinant baculoviruses directing rapid expression of passenger genes in infected insects. Virology 190: 815-823.
19. Lu, M., Farrell, P. J., Johnson, R., Iatrou, K. 1997. A baculovirus (BmNPV) repeat element functions as a powerful constitutive enhancer in transfected insect cells. J. Biol. Chem. 272: 30724-30728.
20. Lu, M., Johnson, R. R., Iatrou, K. 1996. Trans-Activation of a cell housekeeping gene promoter by the IE1 gene product of baculoviruses. Virology 218: 103-113.
21. McCutchen, B. F., Szekacs, A., Huang, T. L., Shiotsuki, T., Hammock, B. D. 1995. Characterization of a spectrophotometric assay for juvenile hormone esterase. Insect Biochem. Molec. Biol. 25: 119-126.
22. Mounier, N., Prudhomme, J. C. 1986. Isolation of actin genes in Bombyx mori. The coding sequence of a cytoplasmic actin gene expressed in the silk gland is interrupted by a single intron in an unusual position. Biochemie 68: 1053-1061.
23. Ogonah, O. W., Freedman, R. B., Jenkins, N., Patel, K., Rooney, B. C. 1996. Isolation and characterization of an insect cell line able to perform complex N-linked glycosylation on recombinant proteins. Bio/ Technology 14: 197-202.
24. O'Reilly, D. R., Miller, L. K., Luckow, V. A. 1992. Baculovirus expression vectors: A laboratory manual. W. H. Freeman and Co., New York.
25. Philpott, M. L., Hammock, B. D. 1990. Juvenile hormone esterase is a biochemical antijuvenile agent. Insect Biochem. 20: 451-459.
26. Rhiel, M., Mitchell-Logean, C. M., Murhammer, D. W. 1997. Comparison of Trichoplusia ni BTI-Tn-5B1-4 (High Five™) and Spodoptera frugiperda Sf-9 insect cell line metabolism in suspension cultures. Biotechnol. Bioeng. 55: 909-920.
27. Rossmann, C., Sharp, N., Allen, G., Gewert, D. 1996. Expression and purification of recombinant, glycosylated human interferon alpha 2b in murine myeloma Nso cells. Prot. Express. Purif. 7: 335-342.
28. Smith, G. E., Summers, M. D., Fraser, M. J. 1993. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol. 3: 2156-2165.
29. Sumathy, S., Palhan, V. S., Gopinathan, K. P. 1996. Expression of human growth hormone in silkworm larvae through recombinant Bombyx mori nuclear polyhedrosis virus. Prot. Express Purif. 7: 262-268.
30. Tkacz, J. S., Lampen, J. O. 1975. Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf liver microsomes. Biochem. Biophys. Res. Commun. 65: 248-255.
31. Vlak, J. M., Klinkenberg, F. A., Zaal, K. J. M., Usmany, N., Klinge-Roode, E. C., Geervliet, J. B. F., Roosien, J., Van Lent, J. W. M. 1988. Functional studies on the p10 gene of Autographa californica nuclear polyhedrosis virus using a recombinant expression of a p10-B-galactosidase fusion gene. J. Gen. Virol. 69: 765-776.
32. Warren, T. J., Hippenmeyer, P. J., Meyer, D. M., Rietz, B. A., Rowald, E. Jr., Carron, C. P. 1994. High-level expression of biologically active, soluble forms of ICAM-1 in a novel mammalian-cell expression system. Prot. Express. Purif. 5: 498-508.
33. Zang, M., Trautmann, H., Gandor, C., Messi, F., Asselbergs, F., Leist, C., Flechter, A., Reiser, J. 1995. Production of recombinant proteins in Chinese hamster ovary cells using a protein-free culture medium. Bio/ Technology 13: 389-392.
34. Zhang, J., Kalogerakis, N., Behie, L. A., Iatrou, K. 1993. A two-stage bioreactor system for the production of recombinant proteins using a genetically engineered baculovirus/insect cell system. Biotechnol. Bioeng. 42: 357-366.