Secreted production of an elastin-like polypeptide by Pichia pastoris

Applied Microbiology and Biotechnology

Volumn 85, Issue 2, 2009, Pages 293-301

  Schipperus, Roelof ^a   Teeuwen, Rosalie L M ^a,b   Werten, Marc W T ^a   Eggink, Gerrit ^a   De Wolf, Frits A ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

Author keywords

Elastin like polypeptides; Heterologous protein expression; Inverse transition cycling; Pichia pastoris; Secreted expression

Indexed keywords

CELL-FREE; CULTURE MEDIUM; ELASTIN-LIKE POLYPEPTIDES; FED-BATCH CULTURES; HETEROLOGOUS PROTEINS; INVERSE TEMPERATURE TRANSITIONS; INVERSE TRANSITION; LARGE SCALE PRODUCTIONS; P. PASTORIS; PH OPTIMA; PH RANGE; PH-DEPENDENT; PICHIA PASTORIS; SHAKE-FLASK CULTURES;

BATCH CELL CULTURE; CELL CULTURE; ESCHERICHIA COLI; GLYCOPROTEINS; METHANOL; PROTEINS; PURIFICATION;

POLYPEPTIDES;

ELASTIN; METHANOL; POLYPEPTIDE;

CHEMICAL PROPERTY; COLIFORM BACTERIUM; PEPTIDE; PH; PROTEIN; YEAST;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; FED BATCH CULTURE; FUNGUS CULTURE; LARGE SCALE PRODUCTION; NONHUMAN; PH; PICHIA PASTORIS; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN SECRETION; TEMPERATURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; DNA, FUNGAL; DRUG STABILITY; ELASTIN; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; METHANOL; MOLECULAR SEQUENCE DATA; PEPTIDES; PICHIA; PROTEIN BIOSYNTHESIS;

ESCHERICHIA COLI; PICHIA PASTORIS;

EID: 71349083655     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2082-9     Document Type: Article

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