Optimization of temperature-glycerol-pH conditions for a fed-batch fermentation process for recombinant hookworm (Ancylostoma caninum) anticoagulant peptide (AcAP-5) production by Pichia pastoris

Enzyme and Microbial Technology

Volumn 24, Issue 7, 1999, Pages 438-445

  Inan, Mehmet ^a   Chiruvolu, Vijay ^a   Eskridge, Kent M ^a   Vlasuk, George P ^b   Dickerson, Kenneth ^b   Brown, Scott ^b   Meagher, Michael M ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b Dendreon   (United States)

Author keywords

Ancylostoma caninum anticoagulant peptide (rAcAP 5); Fed batch fermentation; Pichia pastoris; Recombinant protein; Response surface methodology

Indexed keywords

GLYCEROL; MATHEMATICAL MODELS; PH EFFECTS; POLYNOMIALS; PROTEINS; THERMAL EFFECTS; YEAST;

ANTICOAGULANT PEPTIDES; RESPONSE SURFACE METHODOLOGY (RSM);

FERMENTATION;

ANTICOAGULANT PROTEIN; RECOMBINANT PROTEIN;

ANCYLOSTOMA; ARTICLE; CONTROLLED STUDY; DRUG SYNTHESIS; FERMENTATION; METHODOLOGY; NONHUMAN; PH; PICHIA PASTORIS;

ANCYLOSTOMA CANINUM; PICHIA PASTORIS;

EID: 0344572939     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(98)00161-6     Document Type: Article

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