Expression of a synthetic protein-based polymer (elastomer) gene in Aspergillus nidulans

Applied Microbiology and Biotechnology

Volumn 47, Issue 4, 1997, Pages 368-372

  Herzog, R W ^a,c   Singh, N K ^a   Urry, D W ^b   Daniell, H ^a  

^a AUBURN UNIVERSITY   (United States)

^b UNIVERSITY OF ALABAMA SCHOOL OF MEDICINE   (United States)

^c CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELASTOMER; POLYMER;

ARTICLE; ASPERGILLUS NIDULANS; BIOSYNTHESIS; BIOTECHNOLOGY; CONTROLLED STUDY; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PLASMID;

ASPERGILLUS NIDULANS; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CLONING, MOLECULAR; DNA, FUNGAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, FUNGAL; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN BIOSYNTHESIS; RNA, FUNGAL; RUBBER; TRANSFORMATION, GENETIC;

ASPERGILLUS; EMERICELLA NIDULANS; FUNGI;

EID: 0030983421     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530050942     Document Type: Article

References (30)

1. Ballance DJ, Buxton FP, Turner G (1983) Transformation of Aspergillus nidulans by the ornithine-5′-phosphate decarboxylase gene of Neurospora crassa. Biochem Biophys Res Commun 112: 284-389
2. Chambers JAA, Russo VEA (1986) Isolating RNA is easy and fun. Fungal Genet Newsl 33: 22-25
3. Cullen D, Gray GL, Wilson LJ, Hayenga KJ, Lamsa MH, Rey MW, Norton S, Berka RM (1987) Controlled expression and secretion of bovine chymosin in Aspergillus nidulans. Biotechnology 5: 369-376
4. Daniell H (1995) Producing polymers in plants, bacteria. Inform 6: 1365-1370
5. Daniell H, Guda C, McPherson DT, Zhang X, Urry DW (1996) Hyper expression of a synthetic protein-based polymer gene. Methods Mol Biol 63: (in press)
6. Fogarty WM (1994) Enzymes of the genus Aspergillus. In: (Smith JE (ed) Aspergillus (Biotechnology handbooks, vol 7) Plenum, New York, pp 177-218
7. Guda C, Zhang X, McPherson DT, Xu J, Cherry JH, Urry DW, Daniell H (1995) Hyper expression of an environmentally friendly synthetic polymer gene. Biotechnol Lett 17: 745-750
8. Gurr SJ, Unkles SE, Kinghorn JR (1987) The structure and organization of nuclear genes of filamentous fungi. In: Kinghorn JR (ed) Gene structure in eukaryotic microbes. IRL, Oxford, pp 93-138
9. Gwynne DI, Buxton FP, Williams SA, Garven S, Davies RW (1987a) Genetically engineered secretion of active human interferon and a bacterial endoglucanase from Aspergillus nidulans. Biotechnology 5: 713-719
10. Gwynne DI, Buxton FP, Gleeson MA, Davies RW (1987b) Genetically engineered secretion of foreign proteins from Aspergillus species. In: Burgess R (ed) Protein purification: micro to macro. Liss, New York, pp 355-365
11. Gwynne DI, Buxton FP, Williams SA, Sills AM, Johnstone JA, Buch JK, Guo ZM, Drake D, Westphal M, Davies RW (1989) development of an expression system in Aspergillus nidulans. Biochem Soc Trans 17: 338-340
12. Herzog RW, Daniell H, Singh NK, Lemke PA (1996) A comparative study on transformation of Aspergillus nidulans by microprojectile bombardment of conidia and a more conventional procedure using protoplasts treated with polyethyleneglycol. Appl Microbiol Biotechnol 45: 333-337
13. Kinghorn JR, Unkles SE (1994) Molecular genetics and expression of foreign proteins in the genus Aspergillus. In: Smith JE (ed) Aspergillus (Biotechnology handbooks, vol 7) Plenum, New York, pp 65-100
14. Laemmli UK (1970) Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
15. Lee C, Levin A, Branton D (1987) Copper staining: a five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem 166: 308-312
16. McPherson DT, Xu J, Urry DW (1996) Product purification by reversible phase transition following Escherichia coli expression of genes encoding up to 251 repeats of the elastomeric pentapeptide GVGVP. Protein Expr Purif 7: 51-57
17. Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, Hondel CAMJJ van den (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56: 117-124
18. Punt PJ, Dingemanse MA, Kuyvenhoven A, Soede RDM, Pouwels PH, Hondel CAMJJ van den (1990) Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase. Gene 93: 101-109
19. Punt PJ, Zegers ND, Busscher M, Pouwels PH, Hondel van den CAMJJ (1991) Intracellular and extracellular production of proteins in Aspergillus under the control of expression signals of the highly expressed Aspergillus nidulans gpdA gene. J Biotechnol 17: 19-34
20. Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1: 17-20
21. Roberts IN, Oliver RP, Punt PJ, Hondel van den CAMJJ (1989) Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Curr Genet 15: 177-180
22. Sambrook J, Fritsch EF, Maniatis J (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Coldspring Harbor New York
23. Urry DW (1995) Elastic biomolecular machines. Sci Am 272: 64-69
24. Urry DW, Nicol A, Gowda DC, Hoan LD, McKee A, Williams T, Olsen DB, Cox BA (1993). In: Gebelein CG (ed) Biotechnological polymers: medical, pharmaceutical and industrial applications. Technomic, Atlanta. Ga, pp 82-103
25. Urry DW, McPherson DT, Xu J, Daniell H, Guda C, Gowda DC, Jing N, Parker TM (1996) Protein-based polymeric materials synthesis and properties. In: Salomone JC (ed) The polymeric materials enzyclopedia: synthesis, properties and applications. CRC, Boca Raton, Fla. pp 2412-2429
26. Verdoes JC, Punt PJ, Hondel CAMJJ van den (1995) Molecular genetic strain improvement for the overproduction of fungal proteins by filamentous fungi. Appl Microbiol Biotechnol 43: 195-205
27. Waring RB, May GS, Morris NR (1989) Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulin-coding genes. Gene 79: 119-130
28. Yeh H, Ornstein-Goldstein N, Indik Z, Speppard P, Anderson N, Rosenblom J, Ciclia G, Yoon K, Rosenbloom J (1987) Sequence variation of bovine elastin mRNA due to alternative splicing. Collagen Related Res 7: 235-247
29. Zhang X, Guda C, Datta R, Dute R, Urry DW, Daniell H (1995) Nuclear expression of an environmentally friendly synthetic protein based polymer gene in tobacco cells. Biotechnol Lett 17: 1279-1284
30. Zhang X, Urry DW, Daniell H (1996) Expression of an environmentally friendly synthetic protein-based polymer gene in transgenic tobacco plants. Plant Cell Rep (in press)