Production of recombinant human erythropoietin from Pichia pastoris and its structural analysis

Journal of Applied Microbiology

Volumn 103, Issue 6, 2007, Pages 2084-2094

  Celik E ^a,b   Calik P ^a   Halloran, S M ^c   Oliver, S G ^b  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c ANKARA UNIVERSITY   (Turkey)

Author keywords

AOX1 promoter; Erythropoietin; Expression; Factor Xa; Glycan; His tag; MALDI; Pichia pastoris

Indexed keywords

CLONING; DESORPTION; GLYCOSYLATION; MASS SPECTROMETRY; POLYSACCHARIDES; PROTEINS; PURIFICATION; SODIUM DODECYL SULFATE; STRUCTURAL ANALYSIS; SULFUR COMPOUNDS; YEAST;

AOX1 PROMOTER; ERYTHROPOIETIN; EXPRESSION; FACTOR XA; GLYCANS; HIS-TAG; HUMAN ERYTHROPOIETIN; MALDI; N-LINKED GLYCOSYLATION; PICHIA PASTORIS;

GLYCOPROTEINS;

BLOOD CLOTTING FACTOR 10A; POLYHISTIDINE TAG; PROTEINASE; RECOMBINANT ERYTHROPOIETIN;

DNA; ENZYME ACTIVITY; GENE EXPRESSION; HORMONE; MASS SPECTROMETRY; PROTEIN; RECOMBINATION; YEAST;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

PICHIA PASTORIS;

EID: 36549023117     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03448.x     Document Type: Article

References (38)

1. Anumula, K.R. (2000) High-sensitivity and high-resolution methods for glycoprotein analysis. Anal Biochem 283, 17 26.
2. Bill, R.M., Winter, P.C., McHale, C.M., Hoddges, Y.M., Elder, G.H., Caley, J., Flitsch, S.L., Bicknell, R., et al. (1995) Expression and mutagenesis of recombinant human and murine erythropoietins in Escherichia coli. Biochim Biophys Acta 1261, 35 43.
3. Bradford, M.M. (1976) A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72, 248 254.
4. Burke, D., Dawson, D. Stearns, T. (2000) Methods in Yeast Genetics. New York : Cold Spring Harbor Laboratory Press.
5. Cereghino, G.P.L. Cregg, J.M. (1999) Applications of yeast in biotechnology: protein production and genetic analysis. Curr Opin Biotech 10, 422 427.
6. Cereghino, G.P.L. Cregg, J.M. (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24, 45 66.
7. Cregg, J.M., Cereghino, J.L, Shi, J.Y. Higgins, D.R. (2000) Recombinant protein expression in Pichia pastoris. Mol Biotech 16, 23 52.
8. Dell, A. Morris, H.R. (2001) Glycoprotein structure determination by mass spectrometry. Science 291, 2351 2356.
9. Egrie, J. (1990) The cloning and production of recombinant human erythropoietin. Pharmacotherapy 10, 3S 8S.
10. Elliott, S., Giffin, J., Suggs, S., Lau, E.P.L. Banks, A.R. (1989) Secretion of glycosylated human erythropoietin from yeast directed by the α-factor leader region. Gene 79, 167 180.
11. Fernandez, J.M. Hoeffler, J.P. (1999) Gene Expression Systems: Using Nature for the Art of Expression. San Diego : Academic Press Inc.
12. Gemmill, T.R. Trimble, R.B. (1999) Overview of N- and O-linked oligosaccharide structures found in various yeast species. Biochim Biophys Acta - General Subjects 1426, 227 237.
13. Gorisch, H. (1988) Drop dialysis - time course of salt and protein exchange. Anal Biochem 173, 393 398.
14. Hamilton, S.R., Davidson, R.C., Sethuraman, N., Nett, J.H., Jiang, Y.W., Rios, S., Bobrowicz, P., Stadheim, T.A., et al. (2006) Humanization of yeast to produce complex terminally sialylated glycoproteins. Science 313, 1441 1443.
15. Harvey, D.J. (1993) Quantitative aspects of the matrix-assisted laser-desorption mass-spectrometry of complex oligosaccharides. Rapid Comm Mass Spec 7, 614 619.
16. Harvey, D.J. (1999) Matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates. Mass Spec Rev 18, 349 450.
17. Harvey, D.J. (2005) Structural determination of N-linked glycans by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry. Proteomics 5, 1774 1786.
18. Higuchi, M., Oheda, M., Kuboniwa, H., Tomonoh, K., Shimonaka, Y. Ochi, N. (1992) Role of sugar chains in the expression of the biological activity of human erythropoietin. J Biol Chem 267, 7703 7709.
19. Hirose, M., Kameyama, S. Ohi, H. (2002) Characterization of N-linked oligosaccharides attached to recombinant human antithrombin expressed in the yeast Pichia pastoris. Yeast 19, 1191 1202.
20. Jacobson, L.O., Goldwasser, E. Fried, W. (1957) Role of the kidney in erythropoiesis. Nature 179, 633 634.
21. Jelkmann, W. (1992) Erythropoietin: structure, control of production, and function. Physiological Rev 72, 449 489.
22. Karas, M. Hillenkamp, F. (1988) Laser desorption ionization of proteins with molecular masses exceeding 10000 daltons. Anal Chem 60, 2299 2301.
23. Kim, Y.K., Shin, H.S., Tomiya, N., Lee, Y.C., Betenbaugh, M.J. Cha, H.J. (2005) Production and N-glycan analysis of secreted human erythropoietin glycoprotein in stably transfected Drosophila S2 cells. Biotech Bioeng 92, 452 461.
24. Küster, B., Wheeler, S.F., Hunter, A.P., Dwek, R.A. Harvey, D.J. (1997) Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography. Anal Biochem 250, 82 101.
25. Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature 227, 680.
26. Leehuang, S. (1984) Cloning and expression of human erythropoietin cDNA in Escherichia coli. P Natl Acad Sci Biol 81, 2708 2712.
27. Morelle, W. Michalski, J.C. (2005) The mass spectrometric analysis of glycoproteins and their glycan structures. Curr Anal Chem 1, 29 57.
28. Nagao, M., Inoue, K., Moon, S.K., Masuda, S., Takagi, H., Udaka, S. Sasaki, R. (1997) Secretory production of erythropoietin and the extracellular domain of the erythropoietin receptor by Bacillus brevis: affinity purification and characterization. Biosci Biotechnol Biochem 61, 670 674.
29. Narhi, L.O., Arakawa, T., Aokii, K.H., Elmore, R., Rohde, M.F., Boone, T. Strickland, T.W. (1991) The effect of carbohydrate on the structure and stability of erythropoietin. J Biol Chem 266, 23022 23026.
30. Naven, T.J.P. Harvey, D.J. (1996) Effect of structure on the signal strength of oligosaccharides in matrix-assisted laser desorption/ionization mass spectrometry on time-of-flight and magnetic sector instruments. Rapid Commun Mass Spectr 10, 1361 1366.
31. Papac, D.I., Wong, A. Jones, A.J.S. (1996) Analysis of acidic oligosaccharides and glycopeptides by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Anal Chem 68, 3215 3223.
32. Sambrook, J. Russell, D. (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. New York : Cold Spring Harbor Laboratory Press.
33. Stanley, S.M.R. Poljak, A. (2003) Matrix-assited laser-desorption time-of-flight ionization and high performance liquid chromatography- electrospray ionization mass spectral analyses of two glycosylated recombinant epoetins. J Chromatogr B 785, 205 218.
34. Stübiger, G., Marchetti, M., Nagano, M., Reichel, C., Gmeiner, G. Allmaier, G. (2005) Characterisation of intact recombinant human erythropoietins applied in doping by means of planar gel electrophoretic techniques and matrix-assisted laser desorption/ionisation linear time-of-flight mass spectrometry. Rapid Commun Mass Spectr 19, 728 742.
35. Tarentino, A.L., Gomez, C.M. Plummer, T.H. (1985) Deglycosylation of asparagine-linked glycans by peptide: N-glycosidase F. Biochemistry 24, 4665 4671.
36. Walker, G.M. (1998) Yeast: Physiology and Biotechnology. New York : John Wiley & Sons Inc.
37. Wegner, G. (1990) Emerging applications of the methylotrophic yeasts. FEMS Microbiol Rev 7, 279 283.
38. Zanjani, E.D., Poster, J., Borlington, H., Mann, L.I. Wasserman, L.R. (1977) Liver as the primary site of erythropoietin formation in the fetus. J Lab Clin Med 89, 640.