Size-exclusion HPLC of protein using a narrow-bore column for evaluation of breadmaking quality of hard spring wheat flours

Cereal Chemistry

Volumn 86, Issue 4, 2009, Pages 463-469

  Ohm, Jae Bom ^a   Hareland, Gary ^a   Simsek, Senay ^b   Seabourn, Bradford ^c  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b NORTH DAKOTA STATE UNIVERSITY   (United States)

^c U S DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABSORBANCE; FOOD PRODUCTS; QUALITY CONTROL;

ABSORBANCES; BREADMAKING QUALITY; EXTRACTABLES; NARROW-BORE COLUMN; PROTEIN EXTRACT; QUALITY CHARACTERISTIC; SIZE EXCLUSION; SPRING WHEAT; TOTAL PROTEIN; WHEAT FLOURS;

PROTEINS;

TRITICUM AESTIVUM;

EID: 68849116483     PISSN: 00090352     EISSN: None     Source Type: Journal    
DOI: 10.1094/CCHEM-86-4-0463     Document Type: Article

References (31)

1. AACC International. 2000. Approved Methods of the American Association of Cereal Chemists, 10th Ed. The Association: St. Paul, MN.
2. Bangur, R., Batey, I. L., McKenzie, E., and MacRitchie, F. 1997. Dependence of extensograph parameters on wheat protein composition measured by SE-HPLC. J. Cereal Sci. 25:237-241. (Pubitemid 127368299)
3. Batey, I. L., Gupta, R. B., and MacRitchie, F. 1991. Use of high performance liquid chromatography in the study of wheat flour proteins: An improved chromatographic procedure. Cereal Chem. 68:207-209.
4. Bean, S. R., Lyne, R. K., Tilley, K. A., Chung, O. K., and Lookhart. G. L. 1998. Rapid method for quantitation of insoluble polymeric proteins in flour. Cereal Chem. 75:374-379.
5. Békés, F., Kemény, S., and Morell, M. 2006. An integrated approach to predicting end-product quality of wheat. Eur. J. Agron. 25:155-162. (Pubitemid 44128863)
6. Bietz, J. A. 1984. Analysis of wheat gluten proteins by high-performance liquid chromatography. I. Baker's Dig. 58(1):15-17, 20-21, 32.
7. Borneo, R., and Khan, K. 1999. Protein changes during various stages of breadmaking of four spring wheats: Quantification by size-exclusion HPLC. Cereal Chem. 76:711-717. (Pubitemid 29466496)
8. Gianibelli, M. C., Larroque, O. R., MacRitchie, F., Wrigley, C. W. 2001. Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits. Cereal Chem. 78:635-646. (Pubitemid 33050994)
9. Gupta, R. B., Khan, K., and MacRitchie, F. 1993. Biochemical basis of flour properties in bread wheats. I. Effect of variation in the quantity and size distribution of polymeric protein. J. Cereal Sci. 18:23-41.
10. Huebner, F. R., Nelsen, T. C., Chung, O. K., and Bietz, J. A. 1997. Protein distribution among hard red winter wheat varieties as related to environmental and baking quality. Cereal Chem. 74:123-128.
11. Kuktaitie, R., Larsson, H., and Johansson, E. 2004. Variations in protein composition of wheat flour and its relationship to dough mixing behaviour. J. Cereal Sci. 40:31-39. (Pubitemid 38851962)
12. Labuschagne, M. T., Koen, E., and Dessalegn, T. 2004. Use of size exclusion high-performance chromatography for wheat quality prediction in Ethiopia. Cereal Chem. 81:533-537. (Pubitemid 38924570)
13. Larroque, O. R., and Bekes, F. 2000. Rapid size-exclusion chromatography analysis of molecular size distribution for wheat endosperm protein. Cereal Chem. 77:451-453. (Pubitemid 30471458)
14. Larroque, O. R., Gianibelli, M. C., Gomez Sanchez M., and MacRitchie, F. 2000. Procedure for obtaining stable protein extract of cereal flour and whole mean for size-exclusion HPLC analysis. Cereal Chem. 77:448-450. (Pubitemid 30471457)
15. Lee, L., Ng, P. K. W., and Steffe, J. F. 2002. Biochemical studies of proteins in nondeveloped, partially developed, and developed doughs. Cereal Chem. 79:654-661. (Pubitemid 35034381)
16. Maghirang, E. B., Lookhart, G. L. , Bean, S. R., Pierce, R. O., Xie, F., Caley, M. S., Wilson, J. D., Seabourn, B. W., Ram, M. S., Park, S. H., Chung, O. K., and Dowell, F. E. 2006. Comparison of quality characteristics and breadmaking func tionality of hard red winter and hard red spring wheat. Cereal Chem. 83:520-528. (Pubitemid 44452808)
17. Morel, M.-H., Dehlon, P., Autran, J. C., Leygue, J. P., and Bar-L'Helgouac'h, C. 2000. Effects of temperature, sonica tion time, and power settings on size distribution and extractability of total wheat flour proteins as determined by size-exclusion high-performance liquid chromatography. Cereal Chem. 77:685-691. (Pubitemid 30727560)
18. Neue, U. D. 1997. HPLC columns-Theory technology and practice. Pages 49-53. Wiley: New York.
19. Ohm, J. B., Ross, A. S., Ong, Y.-L., and Peterson, C. J. 2006. Using multivariate techniques to predict wheat-flour dough and noodle characteristics from size exclusion HPLC and RVA data. Cereal Chem. 83:1-9.
20. Ohm, J. B., Ross, A. S., Peterson, C. J., and Ong, Y.-L. 2008. Relationships of high molecular weight glutenin subunit composition and molecular weight distribution of wheat flour protein with water absorption and color characteristics of noo dle dough. Cereal Chem. 85:123-131.
21. Ohm, J. B., Ross, A. S., Peterson, C. J., and Morris, C. F. 2009. Relationships of quality characteristics with size exclusion HPLC chromatogram of protein extract in soft white winter wheats. Cereal Chem. 86:197-203.
22. Park, S. H., Bean, S. R., Chung, O. K., and Seib, P. A. 2006. Levels of protein and protein composition in hard winter wheat flours and relationship to breadmaking. Cereal Chem. 83:418-423.
23. Singh, N. K., Donovan, G. R., Batey, I. L., and MacRitchie, F. 1990a. Use of sonication and size-exclusion high performance liquid chromatography in the study of w heat flour proteins. I. Dissolution of total proteins in the absence of reducing agents. Cereal Chem. 67:150-161.
24. Singh, N. K., Donovan, G. R., and MacRitchie, F. 1990b. Use of sonication and size-exclusion high performance liquid chromatography in the study of wheat flour proteins. II. Relative quantity of glutenin as a measure of breadmaking quality. Cereal Chem. 67:161-170.
25. Skerritt, J. H., Hac, L., and Bekes, F. 19 99. Depolymerization of the glutenin macropolymer during dough mixing: I. Changes in levels, molecular weight distribution, and overall composition. Cereal Chem. 76:395-401. (Pubitemid 29244943)
26. Southan, M., and MacRitchie, F. 1999. Molecular weight distribution of wheat proteins. Cereal Chem. 76:827-836.
27. Suchy, J., Lukow, O. M., and Fu, B. X. 2003. Quantification of monomeric and polymeric wheat proteins and the relationship of protein fractions to wheat quality. J. Sci. Food Agric. 83:1083-1090. (Pubitemid 36941042)
28. Suchy, J., Lukow, O. M., Brown, D., DePauw, D. R., Fox, S., and Humphreys, G. 2007. Rapid assessment of glutenin and gliadin in wheat by UV spectrophotometer. Crop Sci 47:91-99. (Pubitemid 46327839)
29. Weegels,. L., van de Pijpekamp, A. M., Graveland, A., Hamer, R. J., and Schofield, J. D. 1996b. Depolymerisation and repolymerisation of wheat glutenin during dough processing. I. Relationships between glutenin macropolymer content and quality parameters. J. Cereal Sci. 23:103-111. (Pubitemid 126354559)
30. Weegels, P. L., Hamer, R. J., and Schofield, J. D. 1997. Depolymerisation and repolymerisation of wheat glutenin during dough processing. II. Changes in composition. J. Cereal Sci. 25:155-163.
31. Zhu, J., and Khan, K. 2002. Quantitative variation of HMW glutenin subunits from hard red spring wheats grown in different environments. Cereal Chem. 79:783-786. (Pubitemid 35285562)