Amino acid and manganese supplementation modulates the glycosylation state of erythropoietin in a CHO culture system

Biotechnology and Bioengineering

Volumn 96, Issue 3, 2007, Pages 538-549

  Crowell, Christopher K ^a   Grampp, Gustavo E ^b   Rogers, Gary N ^b   Miller, Janis ^b   Scheinman, Robert I ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b AMGEN INC   (United States)

Author keywords

Amino acids; Cell culture; Erythropoietin (EPO); Glycosylation; Manganese

Indexed keywords

AMINO ACIDS; BIOSYNTHESIS; GROWTH KINETICS; MANGANESE; METABOLISM; PROTEINS;

ERYTHROPOIETIN (EPO); GLYCOSYLATION STATE; OLIGOSACCHARIDE;

CELL CULTURE;

AMINO ACID; CARBOHYDRATE; GALACTOSE; GALACTOSYLTRANSFERASE; MANGANESE; RECOMBINANT ERYTHROPOIETIN; SIALIC ACID;

ANIMAL CELL; ARTICLE; CELL METABOLISM; CHO CELL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CORRELATION ANALYSIS; CULTURE MEDIUM; NONHUMAN; PROTEIN GLYCOSYLATION; PROTEIN ISOLATION; PROTEIN SYNTHESIS; SIALYLATION; SUPPLEMENTATION;

AMINO ACIDS; ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; COENZYMES; CRICETINAE; CRICETULUS; ERYTHROPOIETIN, RECOMBINANT; GLYCOSYLATION; HUMANS; MANGANESE; N-ACETYLLACTOSAMINE SYNTHASE; PROTEIN MODIFICATION, TRANSLATIONAL;

EID: 33846927813     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21141     Document Type: Article

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