Histamine H4 receptor-RGS fusion proteins expressed in Sf9 insect cells: A sensitive and reliable approach for the functional characterization of histamine H4 receptor ligands

Biochemical Pharmacology

Volumn 78, Issue 6, 2009, Pages 607-616

  Schneider, Erich H ^a   Seifert, Roland ^b  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Constitutive activity; Fusion protein; Gi proteins; Histamine H4 receptor; RGS protein; Steady state GTPase assay

Indexed keywords

GUANOSINE TRIPHOSPHATASE; HISTAMINE H4 RECEPTOR RGS FUSION PROTEIN; HYBRID PROTEIN; RGS4 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RELIABILITY; SENSITIVITY ANALYSIS; SIGNAL NOISE RATIO; STEADY STATE; WILD TYPE;

ANIMALS; GTP-BINDING PROTEINS; HISTAMINE; HUMANS; INSECTS; LIGANDS; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, HISTAMINE; RECOMBINANT FUSION PROTEINS; RGS PROTEINS; SPODOPTERA; SULFHYDRYL COMPOUNDS; TRANSFECTION;

EID: 67651146336     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bcp.2009.05.015     Document Type: Article

References (44)

1. Hill S.J., Ganellin C.R., Timmerman H., Schwartz J.C., Shankley N.P., Young J.M., et al. International Union of Pharmacology XIII. Classification of histamine receptors. Pharmacol Rev 49 (1997) 253-278
2. Raible D.G., Lenahan T., Fayvilevich Y., Kosinski R., and Schulman E.S. Pharmacologic characterization of a novel histamine receptor on human eosinophils. Am J Respir Crit Care Med 149 (1994) 1506-1511
3. Oda T., Morikawa N., Saito Y., Masuho Y., and Matsumoto S. Molecular cloning and characterization of a novel type of histamine receptor preferentially expressed in leukocytes. J Biol Chem 275 (2000) 36781-36786
4. Morse K.L., Behan J., Laz T.M., West Jr. R.E., Greenfeder S.A., Anthes J.C., et al. Cloning and characterization of a novel human histamine receptor. J Pharmacol Exp Ther 296 (2001) 1058-1066
5. 4) expressed in bone marrow. Mol Pharmacol 59 (2001) 420-426
6. 4R. Biochem Biophys Res Commun 279 (2000) 615-620
7. 4 receptor on human eosinophils-role in eosinophil chemotaxis. J Recept Signal Transduct Res 22 (2002) 431-448
8. 4 receptor mediates chemotaxis and calcium mobilization of mast cells. J Pharmacol Exp Ther 305 (2003) 1212-1221
9. 4 receptors in the central nervous system of human and rat. Brain Res 1250 (2009) 41-48
10. 4 receptor antagonists are superior to traditional antihistamines in the attenuation of experimental pruritus. J Allergy Clin Immunol 119 (2007) 176-183
11. 4 receptor antagonists on experimental colitis in the rat. Eur J Pharmacol 522 (2005) 130-138
12. + T cells. J Immunol 176 (2006) 7062-7070
13. s-adenylate cyclase interactions. FASEB J 5 (1991) 2300-2303
14. 4-receptor. Biochemistry 48 (2009) 1424-1438
15. Seifert R., and Wenzel-Seifert K. Constitutive activity of G-protein-coupled receptors: cause of disease and common property of wild-type receptors. Naunyn Schmiedebergs Arch Pharmacol 366 (2002) 381-416
16. Seifert R., Wenzel-Seifert K., and Kobilka B.K. GPCR-Gα fusion proteins: molecular analysis of receptor-G-protein coupling. Trends Pharmacol Sci 20 (1999) 383-389
17. Wenzel-Seifert K., Hurt C.M., and Seifert R. High constitutive activity of the human formyl peptide receptor. J Biol Chem 273 (1998) 24181-24189
18. o-proteins in Sf9 insect cells. Naunyn Schmiedebergs Arch Pharmacol 378 (2008) 261-274
19. 2. Neurosci Lett 447 (2008) 68-72
20. Willars G.B. Mammalian RGS proteins: multifunctional regulators of cellular signalling. Semin Cell Dev Biol 17 (2006) 363-376
21. Bahia D.S., Sartania N., Ward R.J., Cavalli A., Jones T.L., Druey K.M., et al. Concerted stimulation and deactivation of pertussis toxin-sensitive G proteins by chimeric G protein-coupled receptor-regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity of RGS4. J Neurochem 85 (2003) 1289-1298
22. Berman D.M., Kozasa T., and Gilman A.G. The GTPase-activating protein RGS4 stabilizes the transition state for nucleotide hydrolysis. J Biol Chem 271 (1996) 27209-27212
23. i2α. J Biol Chem 275 (2000) 23693-23699
24. q-coupled receptors. J Biol Chem 274 (1999) 3549-3556
25. Wieland T., and Mittmann C. Regulators of G-protein signalling: multifunctional proteins with impact on signalling in the cardiovascular system. Pharmacol Ther 97 (2003) 95-115
26. i subunits. Proc Natl Acad Sci U S A 93 (1996) 15203-15208
27. q-protein coupling. J Neurochem 80 (2002) 678-696
28. 32P] GMP. Biochim Biophys Acta 562 (1979) 11-31
29. 2 receptors. J Pharmacol Exp Ther 321 (2007) 983-995
30. 2 adrenergic receptor. Evidence for ligand-specific conformational changes. J Biol Chem 270 (1995) 28268-28275
31. 4 receptors. Mol Pharmacol 70 (2006) 604-615
32. Abramow-Newerly M., Roy A.A., Nunn C., and Chidiac P. RGS proteins have a signalling complex: interactions between RGS proteins and GPCRs, effectors, and auxiliary proteins. Cell Signal 18 (2006) 579-591
33. Benians A., Nobles M., Hosny S., and Tinker A. Regulators of G-protein signaling form a quaternary complex with the agonist, receptor, and G-protein. A novel explanation for the acceleration of signaling activation kinetics. J Biol Chem 280 (2005) 13383-13394
34. Zeng W., Xu X., Popov S., Mukhopadhyay S., Chidiac P., Swistok J., et al. The N-terminal domain of RGS4 confers receptor-selective inhibition of G protein signaling. J Biol Chem 273 (1998) 34687-34690
35. i3, is a member of a protein family with a highly conserved core domain. Proc Natl Acad Sci U S A 92 (1995) 11916-11920
36. The Universal Protein Resource (UniProt) 2009. Nucleic Acids Res 2009;37:D169-74.
37. q-proteins. Mol Pharmacol 58 (2000) 954-966
38. Roy A.A., Lemberg K.E., and Chidiac P. Recruitment of RGS2 and RGS4 to the plasma membrane by G proteins and receptors reflects functional interactions. Mol Pharmacol 64 (2003) 587-593
39. G-substituent. J Med Chem 52 (2009) 2623-2627
40. 2 receptor agonists. J Med Chem 51 (2008) 7193-7204
41. Leff P. The two-state model of receptor activation. Trends Pharmacol Sci 16 (1995) 89-97
42. + as universal stabilizer of the inactive (R) state. Receptors Channels 7 (2001) 357-369
43. 2D adrenergic receptors: implications for R and R* states. Pharmacology 61 (2000) 14-21
44. Knight P.J., and Grigliatti T.A. Diversity of G proteins in Lepidopteran cell lines: partial sequences of six G protein alpha subunits. Arch Insect Biochem Physiol 57 (2004) 142-150