The human histamine H2-receptor couples more efficiently to Sf9 insect cell Gs-proteins than to insect cell Gq-proteins: Limitations of Sf9 cells for the analysis of receptor/Gq-protein coupling

Journal of Neurochemistry

Volumn 80, Issue 4, 2002, Pages 678-696

  Houston, Christine ^a   Wenzel Seifert, Katharina ^a   Bürckstümmer, Tilmann ^a   Seifert, Roland ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

Fusion protein; Gq protein; Gs protein; H2 receptor; Regulator of G proteins; Sf9 insect cells

Indexed keywords

ADENYLATE CYCLASE; GQ PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE TRIPHOSPHATE; HISTAMINE H1 RECEPTOR; HISTAMINE H2 RECEPTOR; INSECT PROTEIN; PHOSPHOLIPASE C; STIMULATORY GUANINE NUCLEOTIDE BINDING PROTEIN; THROMBOCYTE ACTIVATING FACTOR RECEPTOR; UNCLASSIFIED DRUG; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; GUANOSINE TRIPHOSPHATASE; HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; HISTAMINE; HYBRID PROTEIN;

ARMYWORM; ARTICLE; BINDING AFFINITY; CALCIUM CELL LEVEL; CONTROLLED STUDY; ENZYME ACTIVATION; INSECT CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING; SIGNAL TRANSDUCTION; STEADY STATE; AMINO ACID SEQUENCE; ANIMAL; BINDING COMPETITION; CALCIUM SIGNALING; CELL LINE; CELL MEMBRANE; DRUG EFFECT; ENZYME SPECIFICITY; GENETICS; HUMAN; IMMUNOBLOTTING; MACROMOLECULE; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN BINDING; RADIOASSAY;

CAVIA PORCELLUS; INSECTA; MAMMALIA; MYTHIMNA UNIPUNCTA; SPODOPTERA; SPODOPTERA FRUGIPERDA; SUS SCROFA;

ADENYLATE CYCLASE; AMINO ACID SEQUENCE; ANIMALS; BINDING, COMPETITIVE; CALCIUM SIGNALING; CELL LINE; CELL MEMBRANE; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEIN ALPHA SUBUNITS, GQ-G11; GTP-BINDING PROTEIN ALPHA SUBUNITS, GS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); HETEROTRIMERIC GTP-BINDING PROTEINS; HISTAMINE; HUMANS; IMMUNOBLOTTING; MACROMOLECULAR SUBSTANCES; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RADIOLIGAND ASSAY; RECEPTORS, HISTAMINE H2; RECOMBINANT FUSION PROTEINS; SPODOPTERA; SUBSTRATE SPECIFICITY;

EID: 0036021470     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0022-3042.2001.00746.x     Document Type: Article

References (86)

1. 2 receptor
2. A single column method for the assay of adenylate cyclase
3. sα fusion protein
4. 2 receptors in insect Sf9 cells
5. Receptor-effector coupling by G proteins
6. Molecular tinkering of G protein-coupled receptors: An evolutionary success
7. Histamine-mediated adenylate cyclase stimulation in human myocardium
8. 2+ influx in all-trans-retinoic acid-differentiated HL-60 cells independently of phospholipase C or adenylyl cyclase
9. Inverse agonist activity of β-adrenergic antagonists
10. BW373U86: A nonpeptidic δ-opioid agonist with novel receptor-G protein-mediated actions in rat brain membranes and neuroblastoma cells
11. Probing of the coupling site of the β-adrenergic receptor. Competition between different forms of the guanyl nucleotide binding protein for interaction with the receptor
12. Octopamine receptor subtypes and their modes of action
13. 1 receptor gene
14. 2 receptors expressed in Sf9 and COS7 cells
15. Purinergic reception by culicine mosquitoes
16. 2 receptor
17. Human polymorphonuclear neutrophils. Pharmacological characterization of histamine receptors mediating the elevation of cyclic AMP
18. G proteins: Transducers of receptor-generated signals
19. sα stimulate adenylate cyclase
20. sα in Escherichia coli. Purification and properties of two forms of the protein
21. 2-adrenoceptor on ligand affinity in transgenic mice
22. The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors
23. 11α. Activation of purified phospholipase C isozymes by Gα subunits
24. Mastoparan, a peptide toxin from wasp venom, mimics receptors by activating GTP-binding regulatory proteins (G proteins)
25. International Union of Pharmacology. XIII. Classification of histamine receptors
26. 2+ release from skeletal muscle sarcoplasmic reticulum
27. Two active molecular phenotypes of the tachykinin NK1 receptor revealed by G-protein fusions and mutagenesis
28. 1 receptor gene
29. 2+ influx pathway in Sf9 insect cells following expression of the transient receptor potential-like (trpl) protein of Drosophila
30. q-mediated signaling by expression of RGS4 or GAIP in mammalian cells
31. Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity
32. 2-receptor with guanidine-type agonists
33. A quantitative analysis of beta-adrenergic receptor interactions: Resolution of high and low affinity states of the receptor by computer modeling of ligand binding data
34. i-proteins in HL-60 membranes indirectly through interaction with nucleoside diphosphate kinase
35. αs defines a novel contact site for β-adrenergic receptors
36. i2
37. 2 histamine receptor to phospholipase C
38. Functional properties of muscarinic receptor subtypes Hm1, Hm3 and Hm5 expressed in Sf9 cells using the baculovirus expression system
39. G proteins endogenously expressed in Sf 9 cells: Interactions with mammalian histamine receptors
40. 2 receptor stably expressed in Chinese hamster ovary cells
41. Molecular pharmacological aspects of histamine receptors
42. 3 receptors: Role of two amino acids in the third transmembrane domain
43. Famotidine treatment of children with autistic spectrum disorders: Pilot research using single subject research design
44. 4 receptors reveals substantial pharmacological species variation
45. sαshort: Consequences for receptor-coupling and adenylyl cyclase activation
46. 3 receptor reveals distinct species pharmacological profiles
47. Famotidine in the management of schizophrenia
48. 2 receptors in Huntington's chorea but not in Parkinson's disease: Comparison with animal models
49. Pharmacological and biochemical characterization of complexes of muscarinic acetylcholine receptor and guanine nucleotide-binding protein
50. Insights into ligand pharmacology using receptor-G-protein fusion proteins
51. 2+ mobilization pathway in human HL-60 promyelocytic leukemia cells
52. q promoted by receptor and GTPase-activating proteins
53. αs-like protein by 5-HTdro1 but lack of coupling to inhibitory G-proteins by 5-HTdro2B
54. The Sf9 cell line as a model for studying insect octopamine-receptors
55. Histamine in brain development and tumors
56. Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells
57. i proteins in the Sf9 insect cell. Characterization of the uncoupled recombinant N-formyl peptide receptor
58. RGS4 inhibits platelet-activating factor receptor phosphorylation and cellular responses
59. GTPase-activating proteins for heterotrimeric G proteins: Regulators of G protein signaling (RGS) and RGS-like proteins
60. sα splice variants
61. + as universal stabilizer of the inactive (R) state
62. 2 receptors with an unique agonist/antagonist profile and induces functional differentiation
63. sα fusion protein
64. sα has properties of a constitutively active receptor
65. α fusion proteins: An approach for the molecular analysis of receptor/G-protein coupling
66. Platelet-activating factor receptor and signal transduction
67. 2 receptors
68. 3H]histamine
69. 2 antagonists
70. 2 agonists
71. 11α is not required for interaction with βγ complex
72. 1 adenosine receptor. Solubilization and characterization of a guanine nucleotide-sensitive form of the receptor
73. 2+ elevation mediated by Mas-7 occurs through membrane pore formation
74. 2 receptors is not sensitive to GTP analogs
75. Thapsigargin, a novel molecular probe for studying intracellular calcium release and storage
76. 125I]iodoaminopotentidine
77. 2 receptor mediating inhibition of arachidonate release and activation of cAMP accumulation
78. αs
79. 2 adrenergic chimeric receptors
80. q-proteins
81. High constitutive activity of the human formyl peptide receptor
82. 3
83. sα
84. G-protein-coupled receptors: Molecular mechanisms involved in receptor activation and selectivity of G-protein recognition
85. Measurement of receptor-stimulated guanosine 5′-O-(γ-thio)triphosphate binding by G proteins
86. q-coupled receptors