High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris

Journal of Biotechnology

Volumn 99, Issue 1, 2002, Pages 51-62

  Boettner, Mewes ^b   Prinz, Bianka ^b   Holz, Caterina ^b   Stahl, Ulf ^a   Lang, Christine ^b  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b Proteinstrukturfabrik   (Germany)

Author keywords

Affinity tags; Heterologous expression; High throughput; Pichia pastoris; Screening; Structural genomics

Indexed keywords

CELL CULTURE; CLONING; DNA; GENES; IMMUNOLOGY; YEAST;

AFFINITY TAGS;

ENZYMES;

FUNGAL PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOMATION; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CULTURE MEDIUM; FEEDING; GENE EXPRESSION; GENOMICS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LYSIS; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN STRUCTURE; REGULATORY MECHANISM; SCREENING;

BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA, COMPLEMENTARY; EXPRESSED SEQUENCE TAGS; FLUOROIMMUNOASSAY; GENE EXPRESSION; GENE EXPRESSION REGULATION, FUNGAL; GLUCOSE; METHANOL; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN ENGINEERING; QUALITY CONTROL; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY;

PICHIA; PICHIA PASTORIS;

EID: 0037048809     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(02)00157-8     Document Type: Article

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