-
1
-
-
0024634880
-
Gene replacement in Dictyostelium: Generation of myosin null mutants
-
Manstein, D.J., Titus, M.A., De Lozanne, A. & Spudich, J.A. Gene replacement in Dictyostelium: Generation of myosin null mutants. EMBO J. 8, 923-932 (1989).
-
(1989)
EMBO J
, vol.8
, pp. 923-932
-
-
Manstein, D.J.1
Titus, M.A.2
De Lozanne, A.3
Spudich, J.A.4
-
2
-
-
0024454883
-
Overproduction of the regulatory subunit of the cAMP-dependent protein kinase blocks the differentiation of Dictyostelium discoideum
-
Simon, M.N., Driscoll, D., Mutzel, R., Part, D., Williams, J. & Veron, M. Overproduction of the regulatory subunit of the cAMP-dependent protein kinase blocks the differentiation of Dictyostelium discoideum. EMBO J. 8, 2039-2043 (1989).
-
(1989)
EMBO J
, vol.8
, pp. 2039-2043
-
-
Simon, M.N.1
Driscoll, D.2
Mutzel, R.3
Part, D.4
Williams, J.5
Veron, M.6
-
3
-
-
0022313127
-
Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium
-
Crowley, T.E., Nelien, W., Gomer, R.H. & Firtel, R.A. Phenocopy of discoidin I-minus mutants by antisense transformation in Dictyostelium. Cell 43, 633-641 (1985).
-
(1985)
Cell
, vol.43
, pp. 633-641
-
-
Crowley, T.E.1
Nelien, W.2
Gomer, R.H.3
Firtel, R.A.4
-
4
-
-
0036179411
-
RNAi in Dictyostelium: The role of RNA-directed RNA polymerases and double-stranded RNase
-
Martens, H., Novotny, J., Oberstrass, J., Steck, T.L., Postlethwait, P. & Nellen, W. RNAi in Dictyostelium: The role of RNA-directed RNA polymerases and double-stranded RNase. Mol. Biol. Cell 13, 445-453 (2002).
-
(2002)
Mol. Biol. Cell
, vol.13
, pp. 445-453
-
-
Martens, H.1
Novotny, J.2
Oberstrass, J.3
Steck, T.L.4
Postlethwait, P.5
Nellen, W.6
-
5
-
-
33750102610
-
Restriction enzyme-mediated integration (REMI) mutagenesis
-
Kuspa, A. Restriction enzyme-mediated integration (REMI) mutagenesis. Methods Mol. Biol. 346, 201-209 (2006).
-
(2006)
Methods Mol. Biol
, vol.346
, pp. 201-209
-
-
Kuspa, A.1
-
6
-
-
0021737746
-
DNA-mediated transformation in Dictyostelium discoideum: Regulated expression of an actin gene fusion
-
Nellen, W., Silan, C. & Firtel, R.A. DNA-mediated transformation in Dictyostelium discoideum: Regulated expression of an actin gene fusion. Mol. Cell. Biol. 4, 2890-2898 (1984).
-
(1984)
Mol. Cell. Biol
, vol.4
, pp. 2890-2898
-
-
Nellen, W.1
Silan, C.2
Firtel, R.A.3
-
7
-
-
0024296379
-
Establishment of a transient expression system for Dictyostelium discoideum
-
Howard, P.K., Ahern, K.G. & Firtel, R.A. Establishment of a transient expression system for Dictyostelium discoideum. Nucleic Acids Res. 16, 2613-2623 (1988).
-
(1988)
Nucleic Acids Res
, vol.16
, pp. 2613-2623
-
-
Howard, P.K.1
Ahern, K.G.2
Firtel, R.A.3
-
8
-
-
0010553022
-
Variables controlling the expression level of exogenous genes in Dictyostelium
-
Pang, K.M., Lynes, M.A. & Knecht, D.A. Variables controlling the expression level of exogenous genes in Dictyostelium. Plasmid 41, 187-197 (1999).
-
(1999)
Plasmid
, vol.41
, pp. 187-197
-
-
Pang, K.M.1
Lynes, M.A.2
Knecht, D.A.3
-
9
-
-
0025675894
-
Establishment of conditions for the transformation of nonaxenic Dictyostelium strains
-
Lloyd, M.M., Ceccarelli, A. & Williams, J.G. Establishment of conditions for the transformation of nonaxenic Dictyostelium strains. Dev. Genet. 11, 391-395 (1990).
-
(1990)
Dev. Genet
, vol.11
, pp. 391-395
-
-
Lloyd, M.M.1
Ceccarelli, A.2
Williams, J.G.3
-
10
-
-
0034639257
-
Efficient transformation of Dictyostelium discoideum with a particle inflow gun
-
Wetterauer, B., Salger, K., Demel, P. & Koop, H. Efficient transformation of Dictyostelium discoideum with a particle inflow gun. Biochim. Biophys. Acta 1499, 139-143 (2000).
-
(2000)
Biochim. Biophys. Acta
, vol.1499
, pp. 139-143
-
-
Wetterauer, B.1
Salger, K.2
Demel, P.3
Koop, H.4
-
11
-
-
0027217068
-
A transformation vector for Dictyostelium discoideum with a new selectable marker bsr
-
Sutoh, K. A transformation vector for Dictyostelium discoideum with a new selectable marker bsr. Plasmid 30, 150-154 (1993).
-
(1993)
Plasmid
, vol.30
, pp. 150-154
-
-
Sutoh, K.1
-
12
-
-
0024671747
-
Hygromycin resistance as a selectable marker in Dictyostelium discoideum
-
Egelhoff, T.T., Brown, S.S., Manstein, D.J. & Spudich, J.A. Hygromycin resistance as a selectable marker in Dictyostelium discoideum. Mol. Cell. Biol. 9, 1965-1968 (1989).
-
(1989)
Mol. Cell. Biol
, vol.9
, pp. 1965-1968
-
-
Egelhoff, T.T.1
Brown, S.S.2
Manstein, D.J.3
Spudich, J.A.4
-
13
-
-
0025984452
-
Bleomycin resistance as a selectabte marker for transformation of the eukaryote
-
Chang, A.C., Hall, R.M. & Williams, K.L. Bleomycin resistance as a selectabte marker for transformation of the eukaryote, Dictyostelium discoideum. Gene 107, 165-170 (1991).
-
(1991)
Dictyostelium discoideum. Gene
, vol.107
, pp. 165-170
-
-
Chang, A.C.1
Hall, R.M.2
Williams, K.L.3
-
14
-
-
0025872009
-
The ble gene of Streptoalloteichus hindustanus as a new selectable marker for Dictyostelium discoideum confers resistance to phleomycin
-
Leiting, B. & Noegel, A.A. The ble gene of Streptoalloteichus hindustanus as a new selectable marker for Dictyostelium discoideum confers resistance to phleomycin. Biochem. Biophys. Res. Commun. 180, 1403-1407 (1991).
-
(1991)
Biochem. Biophys. Res. Commun
, vol.180
, pp. 1403-1407
-
-
Leiting, B.1
Noegel, A.A.2
-
15
-
-
0024365567
-
Complementation of a Dictyostelium discoideum thymidylate synthase mutation with the mouse gene provides a new selectable marker for transformation
-
Chang, A.C., Williams, K.L., Williams, J.G. & Ceccarelli, A. Complementation of a Dictyostelium discoideum thymidylate synthase mutation with the mouse gene provides a new selectable marker for transformation. Nucleic Acids Res. 17, 3655-3661 (1989).
-
(1989)
Nucleic Acids Res
, vol.17
, pp. 3655-3661
-
-
Chang, A.C.1
Williams, K.L.2
Williams, J.G.3
Ceccarelli, A.4
-
16
-
-
0025696839
-
Positive selection for Dictyostelium mutants lacking uridine monophosphate synthase activity based on resistance to 5-fluoro-orotic acid
-
Kalpaxis, D., Werner, H., Boy-Marcotte, E., Jacquet, M. & Dingermann, T. Positive selection for Dictyostelium mutants lacking uridine monophosphate synthase activity based on resistance to 5-fluoro-orotic acid. Dev. Genet. 11, 396-402 (1990).
-
(1990)
Dev. Genet
, vol.11
, pp. 396-402
-
-
Kalpaxis, D.1
Werner, H.2
Boy-Marcotte, E.3
Jacquet, M.4
Dingermann, T.5
-
17
-
-
21844510188
-
Bsr-REMI: An improved method for gene tagging using a new vector in Dictyostelium
-
Morio, T., Adachi, H., Sutoh, K., Yanagisawa, K. & Tanaka, Y. Bsr-REMI: an improved method for gene tagging using a new vector in Dictyostelium. J. Plant Res. 108, 111-114 (1995).
-
(1995)
J. Plant Res
, vol.108
, pp. 111-114
-
-
Morio, T.1
Adachi, H.2
Sutoh, K.3
Yanagisawa, K.4
Tanaka, Y.5
-
18
-
-
0031894669
-
Co-insertional replication is responsible for tandem multimer formation during plasmid integration into the Dictyostelium genome
-
Barth, C., Fraser, O.J. & Fisher, P.R. Co-insertional replication is responsible for tandem multimer formation during plasmid integration into the Dictyostelium genome. Plasmid 39, 141-153 (1998).
-
(1998)
Plasmid
, vol.39
, pp. 141-153
-
-
Barth, C.1
Fraser, O.J.2
Fisher, P.R.3
-
19
-
-
0023472439
-
Two vectors which facilitate gene manipulation and a simplified transformation procedure for Dictyostelium discoideum
-
Early, A.E. & Williams, J.G. Two vectors which facilitate gene manipulation and a simplified transformation procedure for Dictyostelium discoideum. Gene 59, 99-106 (1987).
-
(1987)
Gene
, vol.59
, pp. 99-106
-
-
Early, A.E.1
Williams, J.G.2
-
20
-
-
34250780658
-
Protocols for growth and development of Dictyostelium discoideum
-
Fey, P., Kowal, A.S., Gaudet, P., Pilcher, K.E. & Chisholm, R.L. Protocols for growth and development of Dictyostelium discoideum. Nat. Protoc. 2, 1307-1316 (2007).
-
(2007)
Nat. Protoc
, vol.2
, pp. 1307-1316
-
-
Fey, P.1
Kowal, A.S.2
Gaudet, P.3
Pilcher, K.E.4
Chisholm, R.L.5
-
21
-
-
0014200325
-
Cultivation of Dictyostelium discoideum in axenic medium
-
Sussman, R. & Sussman, M. Cultivation of Dictyostelium discoideum in axenic medium. Biochem. Biophys. Res. Commun. 29, 53-55 (1967).
-
(1967)
Biochem. Biophys. Res. Commun
, vol.29
, pp. 53-55
-
-
Sussman, R.1
Sussman, M.2
-
22
-
-
0014841120
-
Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture
-
Watts, D.J. & Ashworth, J.M. Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem. J. 119, 171-174 (1970).
-
(1970)
Biochem. J
, vol.119
, pp. 171-174
-
-
Watts, D.J.1
Ashworth, J.M.2
-
23
-
-
0030295030
-
Wild-type strains of Dictyostelium discoideum can be transformed using a novel selection cassette driven by the promoter of the ribosomal V18 gene
-
Wetterauer, B. et al. Wild-type strains of Dictyostelium discoideum can be transformed using a novel selection cassette driven by the promoter of the ribosomal V18 gene. Plasmid 36, 169-181 (1996).
-
(1996)
Plasmid
, vol.36
, pp. 169-181
-
-
Wetterauer, B.1
-
24
-
-
0027976354
-
Mutation of the third intracellular loop of the cAMP receptor, cAR1, of Dictyostelium yields mutants impaired in multiple signaling pathways
-
Caterina, M.J., Milne, J.L. & Devreotes, P.N. Mutation of the third intracellular loop of the cAMP receptor, cAR1, of Dictyostelium yields mutants impaired in multiple signaling pathways. J. Biol. Chem. 269, 1523-1532 (1994).
-
(1994)
J. Biol. Chem
, vol.269
, pp. 1523-1532
-
-
Caterina, M.J.1
Milne, J.L.2
Devreotes, P.N.3
-
25
-
-
0026587818
-
Analysis of G alpha 4, a G-protein subunit required for multicellular development in Dictyostelium
-
Hadwiger, J.A. & Firtel, R.A. Analysis of G alpha 4, a G-protein subunit required for multicellular development in Dictyostelium. Genes Dev. 6, 38-49 (1992).
-
(1992)
Genes Dev
, vol.6
, pp. 38-49
-
-
Hadwiger, J.A.1
Firtel, R.A.2
-
26
-
-
0025975846
-
Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid
-
Kalpaxis, D. et al. Positive selection for Dictyostelium discoideum mutants lacking UMP synthase activity based on resistance to 5-fluoroorotic acid. Mol. Gen. Genet. 225, 492-500 (1991).
-
(1991)
Mol. Gen. Genet
, vol.225
, pp. 492-500
-
-
Kalpaxis, D.1
-
27
-
-
19644390035
-
A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system
-
Faix, J., Kreppel, L., Shaulsky, G., Schleicher, M. & Kimmel, A.R. A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system. Nucleic Acids Res. 32, e143 (2004).
-
(2004)
Nucleic Acids Res
, vol.32
-
-
Faix, J.1
Kreppel, L.2
Shaulsky, G.3
Schleicher, M.4
Kimmel, A.R.5
-
28
-
-
0346655253
-
Generation of double gene disruptions in Dictyostelium discoideum using a single antibiotic marker selection
-
Betapudi, V., Shoebotham, K. & Egelhoff, T.T. Generation of double gene disruptions in Dictyostelium discoideum using a single antibiotic marker selection. Biotechniques 36, 106-112 (2004).
-
(2004)
Biotechniques
, vol.36
, pp. 106-112
-
-
Betapudi, V.1
Shoebotham, K.2
Egelhoff, T.T.3
-
29
-
-
0028287369
-
Two cAMP receptors activate common signaling pathways in Dictyostelium
-
Insall, R.H., Soede, R.D., Schaap, P. & Devreotes, P.N. Two cAMP receptors activate common signaling pathways in Dictyostelium. Mol. Biol. Cell 5, 703-711 (1994).
-
(1994)
Mol. Biol. Cell
, vol.5
, pp. 703-711
-
-
Insall, R.H.1
Soede, R.D.2
Schaap, P.3
Devreotes, P.N.4
-
30
-
-
0037664605
-
Dictyostelium discoideum transformation by oscillating electric field electroporation
-
Alibaud, L., Cosson, P. & Benghezal, M. Dictyostelium discoideum transformation by oscillating electric field electroporation. Biotechniques 35, 78-80, 82-83 (2003).
-
(2003)
Biotechniques
, vol.35
-
-
Alibaud, L.1
Cosson, P.2
Benghezal, M.3
-
31
-
-
0030716305
-
Blocking the ends of transforming DNA enhances gene targeting in Dictyostelium
-
Shah-Mahoney, N., Hampton, T., Vidaver, R. & Ratner, D. Blocking the ends of transforming DNA enhances gene targeting in Dictyostelium. Gene 203, 33-41 (1997).
-
(1997)
Gene
, vol.203
, pp. 33-41
-
-
Shah-Mahoney, N.1
Hampton, T.2
Vidaver, R.3
Ratner, D.4
-
32
-
-
0030783741
-
A novel, negative selectable marker for gene disruption in Dictyostelium
-
Morrison, A., Marschalek, R., Dingermann, T. & Harwood, A.J. A novel, negative selectable marker for gene disruption in Dictyostelium. Gene 202, 171-176 (1997).
-
(1997)
Gene
, vol.202
, pp. 171-176
-
-
Morrison, A.1
Marschalek, R.2
Dingermann, T.3
Harwood, A.J.4
-
33
-
-
0004136246
-
-
Sambrook, J, Fritsch, E.F. & Maniatis, T, eds, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
-
Sambrook, J., Fritsch, E.F. & Maniatis, T. (eds.) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1989).
-
(1989)
Molecular Cloning: A Laboratory Manual
-
-
-
34
-
-
0030838576
-
Dictyostelium RasG is required for normal motility and cytokinesis, but not growth
-
Tuxworth, R.I., Cheetham, J.L., Machesky, L.M., Spiegelmann, G.B., Weeks, G. & Insall, R.H. Dictyostelium RasG is required for normal motility and cytokinesis, but not growth. J. Cell Biol. 138, 605-614 (1997).
-
(1997)
J. Cell Biol
, vol.138
, pp. 605-614
-
-
Tuxworth, R.I.1
Cheetham, J.L.2
Machesky, L.M.3
Spiegelmann, G.B.4
Weeks, G.5
Insall, R.H.6
-
35
-
-
0029991350
-
Free in PMC transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation
-
Jordan, M., Schallhorn, A. & Wurm, F.M. Free in PMC transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic Acids Res. 24 596-601 (1996).
-
(1996)
Nucleic Acids Res
, vol.24
, pp. 596-601
-
-
Jordan, M.1
Schallhorn, A.2
Wurm, F.M.3
-
36
-
-
0006500895
-
Mechanisms of DNA uptake by mammalian cells: Fate of exogenously added DNA monitored by the use of fluorescent dyes
-
Loyter, A., Scangos, G.A. & Ruddle, F.H. Mechanisms of DNA uptake by mammalian cells: Fate of exogenously added DNA monitored by the use of fluorescent dyes. Proc. Natl. Acad. Sci. USA 79, 422-426 (1982).
-
(1982)
Proc. Natl. Acad. Sci. USA
, vol.79
, pp. 422-426
-
-
Loyter, A.1
Scangos, G.A.2
Ruddle, F.H.3
|