Secretory expression of glycosylated and aglycosylated mutein of onconase from Pichia pastoris using different secretion signals and their purification and characterization

FEMS Yeast Research

Volumn 9, Issue 4, 2009, Pages 591-599

  Zhao, Hong Liang ^a   He, Qing ^a   Xue, Chong ^a   Sun, Bo ^a   Yao, Xue Qin ^a   Liu, Zhi Min ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

Cytotoxity; Glycosylation; Onconase; Pichia pastoris; Secretion signal

Indexed keywords

RANPIRNASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BREAST CARCINOMA; CELL DENSITY; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CYTOTOXICITY; DRUG MANUFACTURE; ENZYME ACTIVITY; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME STRUCTURE; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; IC 50; IN VITRO STUDY; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION;

ANIMALS; ANTINEOPLASTIC AGENTS; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; PICHIA; PROTEIN SORTING SIGNALS; RANA PIPIENS; RIBONUCLEASES;

PICHIA PASTORIS; RANA PIPIENS;

EID: 65549094527     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/j.1567-1364.2009.00498.x     Document Type: Article

References (42)

1. Ardelt W, Mikulski SM Shogen K (1991) Amino acid sequence of an anti-tumor protein from Rana pipiens oocytes and early embryos. Homology to pancreatic ribonucleases. J Biol Chem 266 : 245 251. (Pubitemid 21906115)
2. Ardelt W, Shogen K Darzynkiewicz Z (2008) Onconase and amphinase, the antitumor ribonucleases from Rana pipiens oocytes. Curr Pharm Biotechno 9 : 215 225.
3. Arnold U, Schulenburg C, Schmidt D Ulbrich-Hofmann R (2006) Contribution of structural peculiarities of onconase to its high stability and folding kinetics. Biochemistry 45 : 3580 3587.
4. Beck AK, Pass HI, Carbone M Yang H (2008) Ranpirnase as a potential antitumor ribonuclease treatment for mesothelioma and other malignancies. Future Oncol 4 : 341 349.
5. Boix E, Wu Y, Vasandani VM, Saxena SK, Ardelt W, Ladner J Youle RJ (1996) Role of the N terminus in RNase A homologues: differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity. J Mol Biol 257 : 992 1007. (Pubitemid 26117085)
6. Cabral KM, Almeida MS, Valente AP, Almeida FC Kurtenbach E (2003) Production of the active antifungal Pisum sativum defensin 1 (Psd1) in Pichia pastoris: overcoming the inefficiency of the STE13 protease. Protein Expres Purif 31 : 115 122. (Pubitemid 37207649)
7. Cereghino GP, Cereghino JL, Ilgen C Cregg JM (2002) Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotech 13 : 329 332. (Pubitemid 35254090)
8. Cregg JM (2007) Introduction: distinctions between Pichia pastoris and other expression systems. Methods Mol Biol 389 : 1 10. (Pubitemid 350189983)
9. Darzynkiewicz Z, Carter SP, Mikulski SM, Ardelt WJ Shogen K (1988) Cytostatic and cytotoxic effects of Pannon (P-30 protein), a novel anticancer agent. Cell Tissue Kinet 21 : 169 182. (Pubitemid 19053361)
10. Fuchs SM, Rutkoski TJ, Kung VM, Groeschl RT Raines RT (2007) Increasing the potency of a cytotoxin with an arginine graft. Protein Eng Des Sel 20 : 505 509. (Pubitemid 350135563)
11. Ghosalkar A, Sahai V Srivastava A (2008) Secretory expression of interferon-alpha 2b in recombinant Pichia pastoris using three different secretion signals. Protein Expres Purif 60 : 103 109.
12. Henrich S, Lindberg I, Bode W Than ME (2005) Proprotein convertase models based on the crystal structures of furin and kexin: explanation of their specificity. J Mol Biol 345 : 211 227. (Pubitemid 39574845)
13. Huang YS, Dong Y, Li H, Wang TY, Qiu JW Yu YN (2001) Purification and characterization of recombinant human interleukin 11 which expressed by Pichia pastoris. Chinese J Biotechnol 17 : 250 253.
14. Ide N, Masuda T Kitabatake N (2007) Effects of pre- and pro-sequence of thaumatin on the secretion by Pichia pastoris. Biochem Bioph Res Co 363 : 708 714. (Pubitemid 47513687)
15. Ilinskaya ON, Koschinski A, Mitkevich VA, Repp H, Dreyer F, Pace CN Makarov AA (2004) Cytotoxicity of RNases is increased by cationization and counteracted by K(Ca) channels. Biochem Bioph Res Co 314 : 550 554. (Pubitemid 38084753)
16. Ita M, Halicka HD, Tanaka T, Kurose A, Ardelt B, Shogen K Darzynkiewicz Z (2008) Remarkable enhancement of cytotoxicity of onconase and cepharanthine when used in combination on various tumor cell lines. Cancer Biol Ther 7 : 1104 1108.
17. Johnson RJ, Chao TY, Lavis LD Raines RT (2007) Cytotoxic ribonucleases: the dichotomy of Coulombic forces. Biochemistry 46 : 10308 10316. (Pubitemid 350067624)
18. Kim BM, Kim H, Raines RT Lee Y (2004) Glycosylation of onconase increases its conformational stability and toxicity for cancer cells. Biochem Bioph Res Co 315 : 976 983. (Pubitemid 38249399)
19. Kjeldsen T, Brandt J, Andersen AS, Egel-Mitani M, Hach M, Pettersson AF Vad K (1996) A removable spacer peptide in an alpha-factor-leader/insulin precursor fusion protein improves processing and concomitant yield of the insulin precursor in Saccharomyces cerevisiae. Gene 170 : 107 112. (Pubitemid 26130804)
20. Lee I, Kalota A, Gewirtz AM Shogen K (2007) Antitumor efficacy of the cytotoxic RNase, ranpirnase, on A549 human lung cancer xenografts of nude mice. Anticancer Res 27 : 299 307. (Pubitemid 46363346)
21. Lee JE Raines RT (2008) Ribonucleases as novel chemotherapeutics: the ranpirnase example. Biodrugs 22 : 53 58.
22. Lee JE, Bae E, Bingman CA, Phillips GN Jr. Raines RT (2008) Structural basis for catalysis by onconase. J Mol Biol 375 : 165 177. (Pubitemid 350160687)
23. Leland PA, Staniszewski KE, Kim B Raines RT (2000) A synapomorphic disulfide bond is critical for the conformational stability and cytotoxicity of an amphibian ribonuclease. FEBS Lett 477 : 203 207. (Pubitemid 30450167)
24. Liao YD, Wang SC, Leu YJ, Wang CF, Chang ST, Hong YT, Pan YR Chen C (2003) The structural integrity exerted by N-terminal pyroglutamate is crucial for the cytotoxicity of frog ribonuclease from Rana pipiens. Nucleic Acids Res 31 : 5247 5255. (Pubitemid 37441892)
25. Lin JJ, Newton DL, Mikulski SM, Kung HF, Youle RJ Rybak SM (1994) Characterization of the mechanism of cellular and cell free protein synthesis inhibition by an anti-tumor ribonuclease. Biochem Bioph Res Co 204 : 156 162. (Pubitemid 24335275)
26. Mikulski SM, Ardelt W, Shogen K, Bernstein EH Menduke H (1990a) Striking increase of survival of mice bearing M109 Madison carcinoma treated with a novel protein from amphibian embryos. J Natl Cancer I 82 : 151 153. (Pubitemid 20034351)
27. Mikulski SM, Viera A, Ardelt W, Menduke H Shogen K (1990b) Tamoxifen and trifluoroperazine (Stelazine) potentiate cytostatic/cytotoxic effects of P-30 protein, a novel protein possessing anti-tumor activity. Cell Tissue Kinet 23 : 237 246. (Pubitemid 20310710)
28. Miller WL (1979) Use of recombinant DNA technology for the production of polypeptides. Adv Exp Med Biol 118 : 153 174.
29. Mosimann SC, Ardelt W James MN (1994) Refined 1.7 A X-ray crystallographic structure of P-30 protein, an amphibian ribonuclease with anti-tumor activity. J Mol Biol 236 : 1141 153. (Pubitemid 124002093)
30. Notomista E, Cafaro V, Fusiello R, Bracale A, D'Alessio G Di Donato A (1999) Effective expression and purification of recombinant onconase, an antitumor protein. FEBS Lett 463 : 211 215. (Pubitemid 30001921)
31. Notomista E, Catanzano F, Graziano G, Dal Piaz F, Barone G, D'Alessio G Di Donato A (2000) Onconase: an unusually stable protein. Biochemistry 39 : 8711 8718. (Pubitemid 30602780)
32. Rodríguez M, Torrent G, Bosch M, Rayne F, Dubremetz JF, Ribó M, Benito A, Vilanova M Beaumelle B (2007) Intracellular pathway of onconase that enables its delivery to the cytosol. J Cell Sci 120 : 1405 1411. (Pubitemid 46780033)
33. Rutkoski TJ Raines RT (2008) Evasion of ribonuclease inhibitor as a determinant of ribonuclease cytotoxicity. Curr Pharm Biotechno 9 : 185 189.
34. Saxena SK, Sirdeshmukh R, Ardelt W, Mikulski SM, Shogen K Youle RJ (2002) Entry into cells and selective degradation of tRNAs by a cytotoxic member of the RNase A family. J Biol Chem 277 : 15142 15146.
35. Schulenburg C, Ardelt B, Ardelt W, Arnold U, Shogen K, Ulbrich-Hofmann R Darzynkiewicz Z (2007) The interdependence between catalytic activity, conformational stability, and cytotoxicity of onconase. Cancer Biol Ther 6 : 1233 1239.
36. Welker E, Hathaway L, Xu G, Narayan M, Pradeep L, Shin HC Scheraga HA (2007) Oxidative folding and N-terminal cyclization of onconase. Biochemistry 46 : 5485 5493. (Pubitemid 46717272)
37. Wu K, Yuan J Lasky LA (1996) Characterization of a novel member of the macrophage mannose receptor type C lectin family. J Biol Chem 271 : 21323 21330. (Pubitemid 26292993)
38. Wu Y, Mikulski SM, Ardelt W, Rybak SM Youle RJ (1993) A cytotoxic ribonuclease. Study of the mechanism of onconase cytotoxicity. J Biol Chem 268 : 10686 10693. (Pubitemid 23150262)
39. Xie YF, Chen H Huang BR (2007) Expression, purification and characterization of human IFN-lambda1 in Pichia pastoris. J Biotechnol 129 : 472 480. (Pubitemid 46560157)
40. Zhao H, Ardelt B, Ardelt W, Shogen K Darzynkiewicz Z (2008a) The cytotoxic ribonuclease onconase targets RNA interference (siRNA). Cell Cycle 7 : 3258 3261.
41. Zhao HL, Xue C, Wang Y, Li XY, Xiong XH, Yao XQ Liu ZM (2007) Circumventing the heterogeneity and instability of human serum albumin-interferon-alpha2b fusion protein by altering its orientation. J Biotechnol 131 : 245 252. (Pubitemid 47362420)
42. Zhao HL, Xue C, Wang Y, Yao XQ Liu ZM (2008b) Increasing the cell viability and heterologous protein expression of Pichia pastoris mutant deficient in PMR1 gene by culture condition optimization. Appl Microbiol Biot 81 : 235 241.