Effective expression and purification of recombinant onconase, an antitumor protein

FEBS Letters

Volumn 463, Issue 3, 1999, Pages 211-215

  Notomista, Eugenio ^a   Cafaro, Valeria ^a   Fusiello, Rossano ^a   Bracale, Aurora ^a   D'Alessio, Giuseppe ^a   Di Donato, Alberto ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Antitumor; Onconase; Recombinant expression; Ribonuclease

Indexed keywords

RANPIRNASE; RECOMBINANT ENZYME; RIBONUCLEASE A; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTINEOPLASTIC ACTIVITY; ARTICLE; CANCER CELL CULTURE; CATALYSIS; CONTROLLED STUDY; CYTOTOXICITY; DOSE RESPONSE; ENZYME PURIFICATION; HUMAN; HUMAN CELL; LEUKEMIA CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; ANTINEOPLASTIC AGENTS; EGG PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION; MUTATION; PROTEIN SYNTHESIS INHIBITORS; RANA PIPIENS; RECOMBINANT PROTEINS; RIBONUCLEASES; RNA, FUNGAL; SODIUM CHLORIDE; TUMOR CELLS, CULTURED;

ESCHERICHIA COLI;

EID: 0033405882     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(99)01623-3     Document Type: Article

References (21)

1. Youle, R.J. and D'Alessio, G. (1997) in: Ribonucleases. Structures and Function (D'Alessio, G. and Riordan, J.F., Eds.), pp. 491-514, Academic Press, San Diego, CA.
2. Juan G., Ardelt B., Li X., Mikulski S.M., Shogen K., Ardelt W., Mittelman A., Darzynkiewicz Z. Leukemia. 12:1998;1241-1248.
3. Boix E., Wu Y., Vasandani V.M., Saxena S.K., Ardelt W., Ladner J., Youle R.J. J. Mol. Biol. 257:1996;992-1007.
4. Newton D.L., Walbridge S., Mikulski S.M., Ardelt W., Shogen K., Ackerman S.J., Rybak S.M., Youle R.J. J. Neurosci. 14:1994;538-544.
5. Leland P.A., Schultz L.W., Kim B.M., Raines R.T. Proc. Natl. Acad. Sci. USA. 95:1998;10407-10412.
6. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Sanger F., Nicklen S., Coulson A.R. Proc. Natl. Acad. Sci. USA. 76:1977;5653-5667.
8. Kunkel T.A. Proc. Natl. Acad. Sci. USA. 82:1987;488-492.
9. Kunitz M. J. Biol. Chem. 164:1946;563-568.
10. Blackburn P., Wilson G., Moore S. J. Biol. Chem. 252:1977;5904-5910.
11. Laemmli U. Nature. 227:1970;680-685.
12. Di Donato A., Cafaro V., Romeo I., D'Alessio G. Protein Sci. 4:1995;1470-1477.
13. Wagner F.W., Wilkes S.H., Prescott J.M. J. Biol. Chem. 247:1972;1208-1210.
14. Shapiro R., Harper J.W., Fox E.A., Jansen H.-W., Hein F., Uhlmann E. Anal. Biochem. 175:1988;450-461.
15. Adinolfi B.S., Cafaro V., D'Alessio G., Di Donato A. Biochem. Biophys. Res. Commun. 213:1995;525-532.
16. Mosimann S.C., Ardelt W., James M.N. J. Mol. Biol. 236:1994;1141-1153.
17. Mosimann S.C., Newton D.L., Youle R.J., James M.N. J. Mol. Biol. 260:1996;540-552.
18. Fontana, A., De Filippis, V., Polverino de Laureto, P., Scaramella, E. and Zambonin, M. (1998) in: Stability and Stabilization of Biocatalysts (Ballestreros, A., Plou, F.J., Iborra, J.L. and Halling, P.J., Eds.), pp. 277-294, Elsevier, Amsterdam.
19. Vescia S., Tramontano D. Mol. Cell. Biochem. 36:1981;125-128.
20. Mastronicola M.R., Piccoli R., D'Alessio G. Eur. J. Biochem. 230:1995;242-249.
21. Piccoli, R. et al. (1999) in: 5th International Meeting on Ribonucleases, Warrenton, VA.