The hydrophobic motif of ROCK2 requires association with the N-terminal extension for kinase activity

Biochemical Journal

Volumn 419, Issue 1, 2009, Pages 141-148

  Couzens, Amber L ^a   Saridakis, Vivian ^a   Scheid, Michael P ^a  

^a YORK UNIVERSITY   (Canada)

Author keywords

Cytoskeleton; Protein kinase; Rho; Rho associated coiled coil kinase (ROCK); Signal transduction

Indexed keywords

ACTIN CYTOSKELETON; ACTIVE COMPLEXES; BLOCK ROCK; C-TERMINAL EXTENSIONS; COILED COIL; CYTOSKELETON; DIMER FORMATION; HYDROPHOBIC CLUSTERS; HYDROPHOBIC MOTIFS; IN-VITRO; KINASE ACTIVITY; KINASE DOMAINS; KINASE FAMILY; N-TERMINAL EXTENSION; PROTEIN KINASE; RHO; RHO-ASSOCIATED COILED-COIL KINASE (ROCK); SMALL-MOLECULE INHIBITORS; STATE TRANSITIONS; THREONINE RESIDUES; TYROSINE RESIDUES;

AMINES; AMINO ACIDS; BACTERIOPHAGES; DIMERIZATION; PHOSPHORYLATION; PRESSURE EFFECTS; ROCKS; SIGNAL TRANSDUCTION;

HYDROPHOBICITY;

GROWTH FACTOR; RHO ASSOCIATED COILED COIL KINASE 2; RHO KINASE; UNCLASSIFIED DRUG; ASPARTIC ACID; ROCK1 PROTEIN, MOUSE; ROCK2 PROTEIN, HUMAN; THREONINE;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL STRAIN 3T3; CELL STRAIN HEK293; CONTROLLED STUDY; DIMERIZATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HELA CELL; HUMAN; HUMAN CELL; HYDROPHOBICITY; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; STRUCTURE ACTIVITY RELATION; ANIMAL; CELL LINE; CHEMISTRY; GENETICS; IMMUNOBLOTTING; IMMUNOPRECIPITATION; METABOLISM; MOUSE; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

AMINO ACID MOTIFS; ANIMALS; ASPARTIC ACID; CELL LINE; DIMERIZATION; HELA CELLS; HUMANS; HYDROPHOBICITY; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MICE; MUTAGENESIS, SITE-DIRECTED; NIH 3T3 CELLS; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RHO-ASSOCIATED KINASES; THREONINE;

EID: 62749110739     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20081376     Document Type: Article

References (27)

1. Ishizaki, T., Maekawa, M., Fujisawa, K., Okawa, K., Iwamatsu, A., Fujita, A., Watanabe, N., Saito, Y., Kakizuka, A., Morii, N. and Narumiya, S. (1996) The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. EMBO J. 15, 1885-1893
2. Matsui, T., Amano, M., Yamamoto, T., Chihara, K., Nakafuku, M., Ito, M., Nakano, T., Okawa, K., Iwamatsu, A. and Kaibuchi, K. (1996) Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho. EMBO J. 15, 2208-2216
3. Leung, T., Chen, X. Q., Manser, E. and Lim, L. (1996) The p160 RhoA-binding kinase ROKα is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Mol. Cell. Biol. 16, 5313-5327
4. Riento, K. and Ridley, A. J. (2003) ROCKs: multifunctional kinases in cell behaviour. Nat. Rev. Mol. Cell Biol. 4, 446-456
5. Maekawa, M., Ishizaki, T., Boku, S., Watanabe, N., Fujita, A., Iwamatsu, A., Obinata, T., Ohashi, K., Mizuno, K. and Narumiya, S. (1999) Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase. Science 285, 895-898
6. Yang, N., Higuchi, O., Ohashi, K., Nagata, K., Wada, A., Kangawa, K., Nishida, E. and Mizuno, K. (1998) Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization. Nature 393, 809-812
7. Kawano, Y., Fukata, Y., Oshiro, N., Amano, M., Nakamura, T., Ito, M., Matsumura, F., Inagaki, M. and Kaibuchi, K. (1999) Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo. J. Cell Biol. 147, 1023-1038
8. Fukata, Y., Amano, M. and Kaibuchi, K. (2001) Rho - Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells. Trends Pharmacol. Sci. 22, 32-39
9. Croft, D. R., Sahai, E., Mavria, G., Li, S., Tsai, J., Lee, W. M. F., Marshall, C. J. and Olson, M. F. (2004) Conditional ROCK activation in vivo induces tumor cell dissemination and angiogenesis. Cancer Res. 64, 8994-9001
10. Sebbagh, M., Renvoize, C., Hamelin, J., Riche, N., Bertoglio, J. and Breard, J. (2001) Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing. Nat. Cell Biol. 3, 346-352
11. Coleman, M. L., Sahai, E. A., Yeo, M., Bosch, M., Dewar, A. and Olson, M. F. (2001) Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I. Nat. Cell Biol. 3, 339-345
12. Newton, A. C. (2003) Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigm. Biochem. J. 370, 361-371
13. Yamaguchi, H., Kasa, M., Amano, M., Kaibuchi, K. and Hakoshima, T. (2006) Molecular mechanism for the regulation of rho-kinase by dimerization and its inhibition by fasudil. Structure 14, 589-600
14. Yang, J., Cron, P., Thompson, V., Good, V. M., Hess, D., Hemmings, B. A. and Barford, D. (2002) Molecular mechanism for the regulation of protein kinase B/Akt by hydrophobic motif phosphorylation. Mol. Cell 9, 1227-1240
15. Biondi, R. M., Kieloch, A., Currie, R. A., Deak, M. and Alessi, D. R. (2001) The PIF-binding pocket in PDK1 is essential for activation of S6K and SGK, but not PKB. EMBO J. 20, 4380-4390
16. Yang, J., Cron, P., Good, V. M., Thompson, V., Hemmings, B. A. and Barford, D. (2002) Crystal structure of an activated Akt/protein kinase B ternary complex with GSK3-peptide and AMP-PNP. Nat. Struct. Biol. 9, 940-944
17. Frodin, M., Antal, T. L., Dummler, B. A., Jensen, C. J., Deak, M., Gammeltoft, S. and Biondi, R. M. (2002) A phosphoserine/ threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation. EMBO J. 21, 5396-5407
18. Biondi, R. M., Cheung, P. C., Casamayor, A., Deak, M., Currie, R. A. and Alessi, D. R. (2000) Identification of a pocket in the PDK1 kinase domain that interacts with PIF and the C-terminal residues of PKA. EMBO J. 19, 979-988
19. Jacobs, M., Hayakawa, K., Swenson, L., Bellon, S., Fleming, M., Taslimi, P. and Doran, J. (2006) The structure of dimeric ROCK I reveals the mechanism for ligand selectivity. J. Biol. Chem. 281, 260-268
20. Balendran, A., Casamayor, A., Deak, M., Paterson, A., Gaffney, P., Currie, R., Downes, C. P. and Alessi, D. R. (1999) PDK1 acquires PDK2 activity in the presence of a synthetic peptide derived from the carboxyl terminus of PRK2. Curr. Biol. 9, 393-404
21. Doran, J. D., Liu, X., Taslimi, P., Saadat, A. and Fox, T. (2004) New insights into the structure-function relationships of Rho-associated kinase: a thermodynamic and hydrodynamic study of the dimer-to-monomer transition and its kinetic implications. Biochem. J. 384, 255-262
22. Engel, M., Hindie, V., Lopez-Garcia, L. A., Stroba, A., Schaeffer, F., Adrian, I., Imig, J., Idrissova, L., Nastainczyk, W., Zeuzem, S. et al. (2006) Allosteric activation of the protein kinase PDK1 with low molecular weight compounds. EMBO J. 25, 5469-5480
23. Kawabata, S., Usukura, J., Morone, N., Ito, M., Iwamatsu, A., Kaibuchi, K. and Amano, M. (2004) Interaction of Rho-kinase with myosin II at stress fibres. Genes Cells 9, 653-660
24. Chen, X. Q., Tan, I., Ng, C. H., Hall, C., Lim, L. and Leung, T. (2002) Characterization of RhoA-binding kinase ROKα implication of the pleckstrin homology domain in ROKα function using region-specific antibodies. J. Biol. Chem. 277, 12680-12688
25. Sahai, E. and Marshall, C. J. (2002) Rho-GTPases and cancer. Nat. Rev. Cancer 2, 133-142
26. Uehata, M., Ishizaki, T., Satoh, H., Ono, T., Kawahara, T., Morishita, T., Tamakawa, H., Yamagami, K., Inui, J., Maekawa, M. and Narumiya, S. (1997) Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature 389, 990-994
27. Shimokawa, H., Seto, M., Katsumata, N., Amano, M., Kozai, T., Yamawaki, T., Kuwata, K., Kandabashi, T., Egashira, K., Ikegaki, I. et al. (1999) Rho-kinase-mediated pathway induces enhanced myosin light chain phosphorylations in a swine model of coronary artery spasm. Cardiovasc. Res. 43, 1029-1039