New insights into the structure-function relationships of Rho-associated kinase: A thermodynamic and hydrodynamic study of the dimer-to-monomer transition and its kinetic implications

Biochemical Journal

Volumn 384, Issue 2, 2004, Pages 255-262

  Doran, John D ^a   Liu, Xun ^a   Taslimi, Paul ^a   Saadat, Ahmad ^a,b   Fox, Ted ^a  

^a VERTEX PHARMACEUTICALS   (United States)

^b ABBOTT LABORATORIES   (United States)

Author keywords

Analytical ultracentrifugation; Dimer; Light scattering; Oligomerization; Protein interactions; Rho associated kinase (ROCK)

Indexed keywords

DIMERS; LIGHT SCATTERING; MONOMERS; OLIGOMERS; PERTURBATION TECHNIQUES; PROTEINS;

OLIGOMERIZATION; ULTRACENTRIFUGATION METHODS;

BIOCHEMISTRY;

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ADENOSINE TRIPHOSPHATE; AMINO ACID; ARGININE; DIMER; LYSYLLYSYLARGINYLASPARAGINYLARGINYLTHREONYLLEUCYLSERYLVALINE; MONOMER; MYOSIN LIGHT CHAIN; PEPTIDE DERIVATIVE; RHO KINASE; SERINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CORRELATION ANALYSIS; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STRUCTURE; HYDRODYNAMICS; LIGHT SCATTERING; MOLECULAR WEIGHT; OLIGOMERIZATION; PRIORITY JOURNAL; SEDIMENTATION RATE; THERMODYNAMICS; ULTRACENTRIFUGATION;

BONE MARROW; BRAIN; CHROMATOGRAPHY, GEL; DIMERIZATION; GENE LIBRARY; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; KINETICS; LEUKOCYTES; LIGHT; LIVER; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP; SCATTERING, RADIATION; THERMODYNAMICS; ULTRACENTRIFUGATION;

FELIS CATUS;

EID: 10644234702     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040344     Document Type: Article

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