Characterization of coenzyme binding and selectivity determinants in Mycobacterium tuberculosis flavoprotein reductase A: Analysis of Arg199 and Arg200 mutants at the NADP(H) 2′-phosphate binding site

Biochemical Journal

Volumn 417, Issue 1, 2009, Pages 103-112

  Sabri, Muna ^a   Dunford, Andrian J ^a   McLean, Kirsty J ^a   Neeli, Rajasekhar ^a   Scrutton, Nigel S ^a   Leys, David ^a   Munro, Andrew W ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Adrenodoxin reductase; Coenzyme binding; Electron transfer; Enzyme mechanism; Flavoprotein reductase A; Mycobacteriumtuberculosis

Indexed keywords

ADRENODOXIN REDUCTASE; COENZYME BINDING; ELECTRON TRANSFER; ENZYME MECHANISM; FLAVOPROTEIN REDUCTASE A; MYCOBACTERIUMTUBERCULOSIS;

AMINO ACIDS; BINDING ENERGY; ELECTRON TRANSITIONS; ENZYMES; KETONES; PHENOLS; RATE CONSTANTS; STRUCTURAL ANALYSIS;

BINDING SITES;

ARGININE; FLAVOPROTEIN REDUCTASE A; HYDROQUINONE; OXIDOREDUCTASE; QUERCETIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; FLAVINE ADENINE NUCLEOTIDE; FPRA PROTEIN, M TUBERCULOSIS; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; AUTOOXIDATION; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; ELECTRON TRANSPORT; ENZYME BINDING; ENZYME MECHANISM; ENZYME SPECIFICITY; MUTATION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; THERMODYNAMICS; CHEMISTRY; COENZYME; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; PROTEIN BINDING; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

MYCOBACTERIUM TUBERCULOSIS;

ARGININE; BINDING SITES; COENZYMES; FLAVIN-ADENINE DINUCLEOTIDE; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTATION; MYCOBACTERIUM TUBERCULOSIS; NADH, NADPH OXIDOREDUCTASES; NADP; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 58249092756     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20080466     Document Type: Article

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