The effect of heating rate on escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: A scale-down study

Biotechnology and Bioengineering

Volumn 102, Issue 2, 2009, Pages 468-482

  Caspeta, Luis ^a   Flores, Noemí ^b   Pérez, Néstor O ^c   Bolívar, Francisco ^b   Ramírez, Octavio T ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b INSTITUTO DE CIENCIAS FÍSICAS   (Mexico)

^c Probiomed SA de CV   (Mexico)

Author keywords

Bacteriophage lambda PL promoter; CI857 repressor; E. Coli; Recombinant protein; Scale down; Scale up; Stress; Temperature induced

Indexed keywords

BACTERIOPHAGE LAMBDA PL PROMOTER; CI857 REPRESSOR; E. COLI; RECOMBINANT PROTEIN; SCALE-DOWN; SCALE-UP; STRESS; TEMPERATURE INDUCED;

BACTERIOPHAGES; BIOCHEMISTRY; CELL CULTURE; ESCHERICHIA COLI; GENES; HEATING RATE; INSULIN; LIGHT EMISSION; LUMINESCENCE; MACHINERY; METABOLISM; PHYSIOLOGICAL MODELS; PHYSIOLOGY; PROTEINS; TRANSCRIPTION; VIRUSES;

HEATING;

ACETIC ACID; CARBOXYLIC ACID; FORMIC ACID; HEAT SHOCK PROTEIN; LACTIC ACID; MESSENGER RNA; PROINSULIN; PROTEIN CLPB; PROTEIN DNAJ; PROTEIN DNAK; RECOMBINANT PROTEIN; SIGMA FACTOR RPOS; TRANSCRIPTION FACTOR RELA; INSULIN; PREPROINSULIN; PROTEIN PRECURSOR;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BIOREACTOR; CONTROLLED STUDY; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; HEATING; NONHUMAN; PHYSIOLOGICAL STRESS; PROTEIN SYNTHESIS; RNA TRANSLATION; BIOSYNTHESIS; CULTURE TECHNIQUE; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HEAT; HUMAN; METABOLISM; METHODOLOGY; MICROBIOLOGY;

BACTERIA (MICROORGANISMS); ENTEROBACTERIA PHAGE LAMBDA; ESCHERICHIA COLI;

BIOREACTORS; CELL CULTURE TECHNIQUES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HEAT-SHOCK PROTEINS; HOT TEMPERATURE; HUMANS; INSULIN; PROTEIN BIOSYNTHESIS; PROTEIN PRECURSORS; RECOMBINANT PROTEINS; RNA, MESSENGER; STRESS, PHYSIOLOGICAL; TRANSCRIPTION, GENETIC;

EID: 58249087221     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22084     Document Type: Article

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