Utility of an Escherichia coli strain engineered in the substrate uptake system for improved culture performance at high glucose and cell concentrations: An alternative to fed-batch cultures

Biotechnology and Bioengineering

Volumn 99, Issue 4, 2008, Pages 893-901

  Lara, Alvaro R ^a   Caspeta, Luis ^a   Gosset, Guillermo ^a   Bolívar, Francisco ^a   Ramírez, Octavio T ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

Acetate production; Batch culture; Fed batch culture; High cell density; Metabolic engineering; Overflow metabolism; PTS; Substrate uptake system

Indexed keywords

BATCH CELL CULTURE; BIOMASS; GLUCOSE SENSORS; METABOLISM; PROTEINS; SUBSTRATES;

ACETATE PRODUCTION; FED BATCH CULTURE; HIGH CELL DENSITY; METABOLIC ENGINEERING; OVERFLOW METABOLISM; PHOSPHOTRANSFERASE SYSTEM (PTS);

ESCHERICHIA COLI;

CARBOHYDRATE; GLUCOSE; GREEN FLUORESCENT PROTEIN; PHOSPHOENOLPYRUVATE; PHOSPHOTRANSFERASE; ESCHERICHIA COLI PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOMASS; CELL METABOLISM; ESCHERICHIA COLI; FED BATCH CULTURE; GLUCOSE BLOOD LEVEL; NONHUMAN; BIOREACTOR; CELL PROLIFERATION; CULTURE TECHNIQUE; GENETIC ENHANCEMENT; GENETICS; METABOLISM; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY;

ESCHERICHIA COLI;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC ENHANCEMENT; GLUCOSE; RECOMBINANT PROTEINS;

EID: 39549119605     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21664     Document Type: Article

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