Comparison of PCR-ELISA and LightCycler real-time PCR assays for detecting Salmonella spp. in milk and meat samples

Molecular and Cellular Probes

Volumn 18, Issue 6, 2004, Pages 409-420

  Perelle, Sylvie ^a   Dilasser, Françoise ^a   Malorny, Burkhard ^b   Grout, Joël ^a   Hoorfar, Jeffrey ^c   Fach, Patrick ^a  

^a Lab d'Etud Rech Sur Qualite A   (France)

^b FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

LightCycler probes; PCR ELISA; Real time PCR; Salmonella

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BACTERIUM DETECTION; COMPARATIVE STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FISH; FOOD ANALYSIS; MEAT; MICROBIOLOGICAL EXAMINATION; MILK; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA; SENSITIVITY AND SPECIFICITY;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FOOD CONTAMINATION; MEAT; MILK; POLYMERASE CHAIN REACTION; SALMONELLA; SEQUENCE ALIGNMENT;

SALMONELLA;

EID: 5644241691     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2004.07.001     Document Type: Article

References (24)

1. B. Malorny, J. Hoorfar, M. Hugas, A. Heuvelink, P. Fach, L. Ellerbroek, C. Bunge, C. Dorn, and R. Helmuth Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method Int J Food Microbiol 89 2003 241 249
2. A.J. Baumler, B.M. Hargis, and R.M. Tsolis Tracing the origins of Salmonella outbreaks Science 287 2000 50 52
3. S.J. Olsen, L.C. MacKinnon, J.S. Goulding, N.H. Bean, and L. Slutsker Surveillance for foodborne-disease outbreaks-United States, 1993-1997 MMWR CDC Surveill Summ 49 2000 1 62
4. T. Humphrey Public-health aspects of Salmonella infection C. Way A. Way Salmonella in domestic animals 2000 CABI Publishing Oxon, UK 245 263
5. C. Tirado, and K. Schmidt WHO surveillance programme for control of foodborne infections and intoxications: results and trends across greater europe J Infect 43 2001 80 84
6. D.J. Wallace, T. Van Gilder, S. Shallow, T. Fiorentino, S.D. Segler, K.E. Smith, B. Shiferaw, R. Etzel, W.E. Garthright, and F.J. Angulo Incidence of foodborne illnesses reported by the foodborne diseases active surveillance network (FoodNet)-1997. FoodNet Working Group J Food Prot 63 2000 807 809
7. K. Rahn, S.A. De Grandis, R.C. Clarke, S.A. McEwen, J.E. Galan, C. Ginocchio, R. Curtiss III, and C.L. Gyles Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella Mol Cell Probes 6 1992 271 279
8. J. Hoorfar, D.L. Baggesen, and P.H. Porting A PCR-based strategy for simple and rapid identification of rough presumptive Salmonella isolates J Microbiol Methods 35 1999 77 84
9. P. Fach, F. Dilasser, J. Grout, and J. Tache Evaluation of a polymerase chain reaction-based test for detecting Salmonella spp. in food samples: Probelia Salmonella spp J Food Prot 62 1999 1387 1393
10. W. Chen, G. Martinez, and A. Mulchandani Molecular beacons: a real-time polymerase chain reaction assay for detecting Salmonella Anal Biochem 280 2000 166 172
11. J. Hoorfar, P. Ahrens, and P. Radstrom Automated 5′ nuclease PCR assay for identification of Salmonella enterica J Clin Microbiol 38 2000 3429 3435
12. A. Eyigor, K.T. Carli, and C.B. Unal Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry Lett Appl Microbiol 34 2002 37 41
13. A. Eyigor, and K.T. Carli Rapid detection of Salmonella from poultry by real-time polymerase chain reaction with fluorescent hybridization probes Avian Dis 47 2003 380 386
14. A.A. Bhagwat Simultaneous detection of Escherichia coli O157:H7. Listeria monocytogenes and Salmonella strains by real-time PCR Int J Food Microbiol 84 2003 217 224
15. A.A. Bhagwat Rapid detection of Salmonella from vegetable rinse-water using real-time PCR Food Microbiol 21 2004 73 78
16. P.M. Fratamico Comparison of culture, polymerase chain reaction (PCR), TaqMan Salmonella, and Transia Card Salmonella assays for detection of Salmonella spp. In naturally-contaminated ground chicken, ground turkey, and ground beef Mol Cell Probes 17 2003 215 221
17. D. Rodriguez-Lazaro, M. Hernandez, T. Esteve, J. Hoorfar, and M. Pla A rapid and direct real time PCR-based method for identification of Salmonella spp J Microbiol Methods 54 2003 381 390
18. M. Uyttendaele, K. Vanwildemeersch, and J. Debevere Evaluation of real-time PCR vs automated ELISA and a conventional culture method using a semi-solid medium for detection of Salmonella Lett Appl Microbiol 37 2003 386 391
19. D. De Medici, L. Croci, E. Delibato, S. Di Pasquale, E. Filetici, and L. Toti Evaluation of DNA extraction methods for use in combination with SYBR green I real-time PCR to detect Salmonella enterica serotype enteritidis in poultry Appl Environ Microbiol 69 2003 3456 3461
20. J.S. Van Kessel, J.S. Karns, and M.L. Perdue Using a portable real-time PCR assay to detect Salmonella in raw milk J Food Prot 66 2003 1762 1767
21. B. Malorny, J. Hoorfar, C. Bunge, and R. Helmuth Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard Appl Environ Microbiol 69 2003 290 296
22. Anonymous Microbiology of food and animal feeding stuffs - horizontal method for the detection of Salmonella (ISO 6579: 2003) 2003 International Organization for Standardization Geneva, Switzerland
23. P. Fach, S. Perelle, F. Dilasser, and J. Grout Comparison between a PCR-ELISA test and the vero cell assay for detecting Shiga toxin-producing Escherichia coli in dairy products and characterization of virulence traits of the isolated strains J Appl Microbiol 90 2001 809 818
24. J. Hoorfar, N. Cook, B. Malorny, M. Wagner, D. De Medici, A. Abdulmawjood, and P. Fach Diagnostic PCR making internal amplification control mandatory J Appl Microbiol 96 2004 221 222