Diagnostic PCR: Making internal amplification control mandatory

Journal of Applied Microbiology

Volumn 96, Issue 2, 2004, Pages 221-222

  Hoorfar, J ^a   Cook, N ^b   Malorny, B ^c   Wagner, M ^d   De Medici, D ^e   Abdulmawjood, A ^f   Fach, P ^g  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b CENTRAL SCIENCE LABORATORY   (United Kingdom)

^c FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

^d Institute for Milk Hygiene   (Austria)

^e ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^f JUSTUS LIEBIG UNIVERSITY   (Germany)

^g Healthy Pigs Production and Experimentation Section   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION CONTROL; DIAGNOSTIC PCR; INTERNAL AMPLIFICATION;

DNA POLYMERASE;

POLYMERASE CHAIN REACTION;

DIAGNOSTIC PROCEDURE; FOOD; GENE AMPLIFICATION; LABORATORY; LETTER; MOLECULAR DYNAMICS; PATHOGENESIS; POLYMERASE CHAIN REACTION; QUALITY CONTROL; REPRODUCIBILITY; SAMPLING; TEMPERATURE;

EID: 1242284358     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2003.02188.x     Document Type: Letter

References (7)

1. Anon. (2001) Protocol for the Validation of Alternative Microbiological Qualitative Methods. Document no. NV-DOC.D - 2001-04-25, published by NordVal: http://nmkl.org/NordVal/NodVal.htm.
2. Anon. (2002a) Microbiology of Food and Animal Feeding Stuffs - Protocol for the Validation of Alternative Methods (EN ISO 16140). European Committee for Standardization, AFNOR, Paris, France.
3. Anon. (2002b) Microbiology of Food and Animal Feeding Stuffs -Polymerase Chain Reaction (PCR) for the Detection of Foodborne Pathogens - General Method Specific Requirements, Draft International Standard ISO/DIS 22174. Berlin, Germany.
4. Hoorfar, J. (1999) EU seeking to validate and standardize PCR testing of food pathogens. ASM News 65, 799.
5. Hoorfar, J. and Cook, N. (2002) Critical aspects of standardization. In PCR Detection of Microbial Pathogens: Methods and Protocols. Methods in Molecular Biology, vol. 216. ed. Sachse, K. and Frey, J. pp. 51-64. Totowa, NJ: Humana Press Inc.
6. Malorny, B., Tassios, P.T., Rådström, P., Cook, N., Wagner, M. and Hoorfar, J. (2003) Standardization of diagnostic PCR for the detection of foodborne pathogens. International Journal of Food Microbiology 83, 39-48.
7. Schoder, D., Schmalwieser, A., Schauberger, G., Kuhn, M., Hoorfar, J. and Wagner, M. (2003) Physical characteristics of six new thermocyclers. Clinical Chemistry 49, 960-963.