Evaluation of DNA extraction methods for use in combination with SYBR green I real-time PCR to detect Salmonella enterica serotype Enteritidis in poultry

Applied and Environmental Microbiology

Volumn 69, Issue 6, 2003, Pages 3456-3461

  De Medici, Dario ^a,b   Croci, Luciana ^a   Delibato, Elisabetta ^a   Di Pasquale, Simona ^a   Filetici, Emma ^a   Toti, Laura ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b Laboratorio Alimenti   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; DNA; DYES; EXTRACTION; PURIFICATION;

BINDING DYE;

BACTERIA;

DNA; DYE;

DETECTION METHOD; DNA; PATHOGEN; POLYMERASE CHAIN REACTION; POULTRY;

AMPLICON; ARTICLE; DNA EXTRACTION; DNA PURIFICATION; LYSIS; MELTING POINT; NONHUMAN; POLYMERASE CHAIN REACTION; POULTRY; SALMONELLA ENTERICA; TEMPERATURE;

GALLUS GALLUS; NEGIBACTERIA; SALMONELLA; SALMONELLA ENTERICA;

EID: 0037978976     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.6.3456-3461.2003     Document Type: Article

References (38)

1. Aarts, H. J., R. G. Joosten, M. H. Henkens, H. Stegeman, and A. H. van Hoek. 2001. Rapid duplex PCR assay for the detection of pathogenic Yersinia enterocolitica strains. J. Microbiol. Methods 47:209-217.
2. Alterkruse, S. F., L. K. Tollefson, and K. Bögel. 1993. Control strategies for Salmonella enteritidis in five countries. Food Control 4:10-16.
3. Bassler, H. A., S. J. A. Flood, K. J. Livak, J. Marmaro, R. Knorr, and C. A. Batt. 1995. Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes. Appl. Envirgn. Microbiol. 61:3724-3728.
4. Baumler, A. J., B. M. Hargis, and R. M. Tsolis. 2000. Tracing the origins of Salmonella outbreaks. Science 287:50-52. [Online.] http://www.sciencemag.org/cgi/content/full/287/5450/50.
5. Chen, S., A. Yee, M. Griffiths, C. Larkin, C. T. Yamashiro, R. Behari, C. Paszko-Kolva, K. Rahn, and S. A. De Grandis. 1997. The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities. Int. J. Food Microbiol. 35:239-250.
6. Davies, R., M. Breslin, J. E. Corry, W. Hudson, and V. M. Allen. 2001. Observations on the distribution and control of Salmonella species in two integrated broiler companies. Vet. Rec. 149:227-232.
7. De Medici, D., G. Pezzotti, C. Marfoglia, D. Caciolo, G. Foschi, and L. Orefice. 1998. Comparison between ICS-Vidas, MSRV and standard cultural method for Salmonella recovery in poultry meat. Int. J. Food Microbiol. 45:205-210.
8. Doran, J. L., S. K. Collinson, S. C. Clouthier, T. A. Cebula, W. H. Koch, J. Burian, P. A. Banser, E. C. Todd, and W. W. Kay. 1996. Diagnostic potential of sefA DNA probes to Salmonella enteritidis and certain other O-serogroup D1 Salmonella serovars. Mol. Cell. Probes 10:233-246.
9. Egygor, A., K. T. Carli, and C. B. Unal. 2002. Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. Lett. Appl. Microbiol. 34:37-41.
10. Gibson, U. E., C. A. Heid, and P. M. Williams. 1996. A novel method for real time quantitative RT-PCR. Genome Res. 6:995-1001.
11. Hargis, B. M., D. J. Caldwell, R. L. Brewer, D. E. Corrier, and J. R. Deloach. 1995. Evaluation of the chicken crop as a source of Salmonella contamination for broiler carcasses. Poult. Sci. 74:1548-1552.
12. Hofstra, H., J. M. van der Vossen, and J. van der Plas. 1994. Microbes in food processing technology. FEMS Microbiol. Rev. 15:175-183.
13. Hoorfar, J., P. Ahrens, and P. Rådström. 2000. Automated 5′ nuclease PCR assay for identification of Salmonella enterica. J. Clin. Microbiol. 38:3429-3435.
14. Kaiser, K., M. Rabodonirina, and S. Picot. 2001. Real time quantitative PCR and RT-PCR for analysis of Pneumocystis carinii hominis. J. Microbiol. Methods 45:113-118.
15. Lantz, P. G., B. Hahn Hagerdal, and P. Rådström. 1994. Sample preparation methods in PCR-based detection of food pathogens. Trends Food Sci. Technol. 5:384-389.
16. Li, X., N. Boudjellab, and X. Zhao. 2000. Combined PCR and slot blot assay for detection of Salmonella and Listeria monocytogenes. Int. J. Food Microbiol. 56:167-177.
17. Mackay, I. M., K. E. Arden, and A. Nitsche. 2002. Real-time PCR in virology. Nucleic Acids Res. 30:1292-1305.
18. Meer, R. R., and D. L. Park. 1995. Immunochemical detection methods for Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes in foods. Rev. Environ. Contam. Toxicol. 142:1-12.
19. Olsen, S. J., L. C. MacKinnon, J. S. Goulding, N. H. Bean, and L. Slutsker. 2000. Surveillance for foodborne-disease outbreaks - United States, 1993-1997. Morb. Mortal. Wkly. Rep. 49:1-62.
20. Rabsch, W., H. Tschäpe, and A. J. Bäumler. 2001. Non-typhoidal salmonellosis: emerging problems. Microbes Infect. 3:237-247.
21. Rijpens, N., L. Herman, F. Vereecken, G. Jannes, J. De Smedt, and L. De Zutter. 1999, Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCR. Int. J. Food Microbiol. 46:37-44.
22. Ririe, K. M., R. P. Rasmussen, and C. T. Wittwer. 1997. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245:154-160.
23. Romero, C., and I. Lopez-Gońi. 1999. Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR. Appl. Environ. Microbiol. 65:3735-3737.
24. Ruzsovics, A., B. Molnar, Z. Unger, Z. Tulassay, and L. Pronai. 2001. Determination of Helicobacter pylori cagA, vacA genotypes with real-time PCR melting curve analysis. J. Physiol. Paris 95:369-377.
25. Scheu, P. M., K. Berghof, and U. Stahl. 1998. Detection of pathogenic and spoilage microorganisms in food with the polymerase chain reaction. Food Microbiol. 15:13-31.
26. Scuderi, G., M. Fantasia, E. Filetici, and M. P. Anastasio. 1996. Foodborne outbreaks caused by salmonella in Italy, 1991-4. Epidemiol. Infect. 116:257-265.
27. Scuderi, G., and S. Gabriella. 2000. A review of the salmonellosis surveillance systems in Italy: evolution during the course of time within the international framework. Eur. J. Epidemiol. 16:861-868.
28. Singer, V. L., T. E. Lawlor, and S. Yue. 1999. Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test). Mutat. Res. 439:37-47.
29. Suzuky, S. 1994. Pathogenicity of Salmonella enteritidis in poultry. Int. J. Food Microbiol. 21:89-105.
30. Szabo, E. A., and B. M. Mackey. 1999. Detection of Salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR). Int. J. Food Microbiol. 51:113-122.
31. Turcotte, C., and M. J. Woodward. 1993. Cloning, DNA nucleotide sequence and distribution of the gene encoding the SEF14 fimbrial antigen of Salmonella enteritidis. J. Gen. Microbiol. 139:1477-1485.
32. Van de Giessen, A. W., A. J. H. A. Ament, and S. H. W. Notermans. 1994. Intervention strategies for Salmonella enteritidis in poultry flocks: a basic approach. Int. J. Food Microbiol. 21:145-154.
33. Vaneechoutte, M., and J. Van Eldere. 1997. The possibilities and limitations of nucleic acid amplification technology in diagnostic microbiology. J. Med. Microbiol. 46:188-194.
34. Vishnubhatla, A., D. Y. C. Fung, R. D. Oberst, M. P. Hays, T. G. Nagaraja, and S. J. Flood. 2000. Rapid 5′ nuclease (TaqMan) assay for detection of virulent strains of Yersinia enterocolitica. Appl. Environ. Microbiol. 66:4131-4135.
35. Wawerla, M., A. Stolle, B. Schalch, and H. Eisgruber. 1999. Impedance microbiology: applications in food hygiene. J. Food Prot. 62:1488-1496.
36. Wilhelm J.M., Hahn, and A. Pingoud. 2000. Influence of DNA target melting behavior on real-time PCR quantification. Clin. Chem. 46:1738-1743.
37. Woodward, M. J., and S. E. Kirwan. 1996. Detection of Salmonella enteritidis in eggs by the polymerase chain reaction. Vet. Rec. 138:411-413.
38. World Health Organization. 1990. Report on WHO consultation on salmonellosis control in agriculture, Orvieto Italy, 9-12 April 1990. VPH/SCA/90. World Health Organization, Geneva, Switzerland.