The structure of a transcribing T7 RNA polymerase in transition from initiation to elongation

Science

Volumn 322, Issue 5901, 2008, Pages 553-557

  Durniak, Kimberly J ^a   Bailey, Scott ^a,b   Steitz, Thomas A ^a  

^a YALE UNIVERSITY   (United States)

^b JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE; TRANSCRIBING T7 RNA POLYMERASE; UNCLASSIFIED DRUG;

ENZYME ACTIVITY; RNA;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME STRUCTURE; GENE MUTATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN; RNA SYNTHESIS; RNA TRANSCRIPTION; TRANSCRIPTION INITIATION;

BACTERIOPHAGE T7; CRYSTALLOGRAPHY, X-RAY; DNA; DNA-DIRECTED RNA POLYMERASES; MODELS, GENETIC; MODELS, MOLECULAR; MUTANT PROTEINS; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 54949100000     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1163433     Document Type: Article

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37. We thank the staff at the Advanced Photon Source beamline 24-ID and at the National Synchrotron Light Source beamline X25. We also thank T. Jorgensen for help with the supplemental movie and the staff of the Center for Structural Biology core facility at Yale University. This work was supported by NIH grant GM57510 (to T.A.S.). The coordinates have been deposited in the Research Collaboratory for Structural Bioinformatics Protein Data Bank with accession numbers 3E2E and 3E3J for the 7- and 8-nt RNA intermediate complex structures, respectively.