Structural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms

Science

Volumn 303, Issue 5660, 2004, Pages 983-988

  Bushnell, David A ^a   Westover, Kenneth D ^a   Davis, Ralph E ^a   Kornberg, Roger D ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ENZYMES; MOLECULAR STRUCTURE;

ABORTIVE INITIATION; TRANSCRIPTION;

RNA;

DNA; RNA POLYMERASE II; TATA BINDING PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR IIB; TRANSCRIPTION FACTOR IIE; TRANSCRIPTION FACTOR IIF; TRANSCRIPTION FACTOR IIH; UNCLASSIFIED DRUG;

RNA;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; DNA DENATURATION; DNA PROTEIN COMPLEX; DNA RNA HYBRIDIZATION; ENZYME ACTIVE SITE; GENETIC TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PROTEIN INTERACTION; TRANSCRIPTION INITIATION;

AMINO ACID SEQUENCE; BINDING SITES; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DNA; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; NUCLEIC ACID HYBRIDIZATION; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RNA; RNA POLYMERASE II; SACCHAROMYCES CEREVISIAE PROTEINS; TATA BOX; TATA-BOX BINDING PROTEIN; TEMPLATES, GENETIC; TRANSCRIPTION FACTOR TFIIB; TRANSCRIPTION FACTORS, TFII; TRANSCRIPTION, GENETIC; ZINC;

RUMEX;

EID: 1142274214     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1090838     Document Type: Article

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49. We thank N. Thompson and R. Burgess for generously providing antibody for protein purification, P. Jadzinsky for help with EM images, and HP for providing a Unix workstation. Portions of this research were carried out at the Stanford Synchrotron Radiation Laboratory (SSRL), a national user facility operated by Stanford University on behalf of the U.S. Department of Energy, Office of Basic Energy Sciences. The SSRL Structural Molecular Biology Program is supported by the Department of Energy, Office of Biological and Environmental Research, and by the National Institutes of Health, National Center for Research Resources, Biomedical Technology Program, and the National Institute of General Medical Sciences. We thank the staff of the Berkeley Center for Structural Biology for help at the Advanced Light Source. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, Materials Sciences Division, of the U.S. Department of Energy under Contract No. DE-AC03-76SF00098 at Lawrence Berkeley National Laboratory. D.A.B. was supported by postdoctoral fellowship PF-00-014-01-GMC from the American Cancer Society. K.D.W. was supported by Medical Scientist Training Program. This research was supported by NIH grants GM4998S and AI21144 to R.D.K. Structure factors and coordinates have been deposited at the protein data bank (accession code 1R5U).