T7 promoter release mediated by DNA scrunching

EMBO Journal

Volumn 20, Issue 23, 2001, Pages 6826-6835

  Brieba, Luis G ^a   Sousa, Rui ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

DNA scrunching; Promoter release; T7RNAP; Transcription initiation

Indexed keywords

DNA; EXONUCLEASE; PERMANGANATE POTASSIUM; RNA; RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE T7; DISSOCIATION; DNA FOOTPRINTING; DNA PROTEIN COMPLEX; PRIORITY JOURNAL; PROMOTER REGION; RNA STRUCTURE; TRANSCRIPTION INITIATION;

BINDING SITES; DNA; DNA-DIRECTED DNA POLYMERASE; EXONUCLEASES; NUCLEIC ACID CONFORMATION; POTASSIUM PERMANGANATE; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; RNA; TRANSCRIPTION, GENETIC;

EID: 0035803474     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.23.6826     Document Type: Article

References (32)

1. Peculiar 2-aminoapurine fluorescence monitors the dynamics of open complex formation by bacteriophage T7 RNA polymerase
2. Specific binding of monomeric bacteriophage T3 and T7 RNA polymerase to their respective cognate promoters requires the initiating ribonucleoside triphosphate (GTP)
3. The T7 RNA polymerases intercalating hairpin is important for promoter opening during initiation but not for RNA displacement of transcription bubble stability during elongation
4. Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lac UV5 promoter
5. Interaction of RNA polymerase with lac UV5 promoter DNA during mRNA initiation and elongation. Footprinting, methylation and rifampicin-sensitivity changes accompanying transcription initiation
6. Structure of a transcribing T7 RNA polymerase initiation complex
7. The stability of abortively cycling T7 RNA polymerase complexes depends upon template conformation
8. Stalling of Escherichia coli RNA polymerase in the +6 to +12 region in vivo is associated with tight binding to consensus promoter elements
9. Interactions of T7 RNA polymerase with T7 late promoters measured by footprinting with methidiumpropyl-EDTA-iron (II)
10. Role of sigma subunit of Escherichia coli RNA polymerase initiation. II. Release of sigma from ternary complexes
11. T7 RNA polymerase elongation complex structure and movement
12. Mechanisms by which T7 lysozyme specifically regulates T7 RNA polymerase during different phases of transcription
13. Misincorporation by wild-type and mutant T7 RNA polymerase: Identification of interactions that reduce misincorporation rates by stabilizing the catalytically incompetent open conformation
14. Interactions of the RNA polymerase of bacteriophage T7 with its promoter during binding and initiation of transcription
15. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants
16. Kinetic mechanism of transcription initiation by bacteriophage T7 RNA polymerase
17. Interrupting the template strand of the T7 promoter facilitates translocation of the DNA during initiation, reducing transcript slippage and the release of abortive products
18. A structural model of transcription elongation
19. RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes
20. Processivity in early stages of transcription by T7 RNA polymerase
21. RNA polymerases unveiled
22. T7 RNA polymerase interacts with its promoter from one side of the DNA helix
23. Studies of contacts between T7 RNA polymerase and its promoter reveals features in common with multisubunit RNA polymerases
24. Polymerase arrest at the λP (R) promoter during transcription initiation
25. Intermediates in transcription initiation from the E.coli lac UV5 promoter
26. A stressed intermediate in the formation of stability initiated RNA chains at the Escherichia coli lac UV5 promoter
27. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes
28. The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance
29. Abortive cycling and the release of polymerase for elongation at the sigma 54-dependent glnAp2 promoter
30. The pUC plasmids and M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
31. Role of complex instability in kinetic promoter selection by bacteriophage T7 RNA polymerase
32. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 Å resolution