Structural transitions mediating transcription initiation by T7 RNA polymerase

Cell

Volumn 110, Issue 1, 2002, Pages 81-91

  Mukherjee, Srabani ^a   Brieba, Luis G ^a   Sousa, Rui ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; HYDROGEN PEROXIDE; NUCLEIC ACID; PROTEIN; RNA; RNA POLYMERASE;

ARTICLE; COMPLEX FORMATION; CONFORMATION; CONJUGATE; CONJUGATION; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME STRUCTURE; GENETIC TRANSCRIPTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN INTERACTION; PROTEIN PROTEIN INTERACTION; PROTEIN SECRETION; TRANSCRIPTION INITIATION;

EID: 0037067648     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(02)00815-2     Document Type: Article

References (41)

1. Structure of DNA polymerase I Klenow fragment bound to duplex DNA
2. T7 promoter release mediated by DNA scrunching
3. The T7 RNA polymerase intercalating hairpin is important for promoter opening during initiation but not for RNA displacement or transcription bubble stability during elongation
4. Scanning mutagenesis reveals roles for helix n of the bacteriophage T7 RNA polymerase thumb subdomain in transcription complex stability, pausing, and termination
5. Cycling of ribonucleic acid polymerase to produce oligonucleotides during initiation in vitro at the lac UV5 promoter
6. Structure of a transcribing T7 RNA polymerase initiation complex
7. Structural basis for initiation of transcription from an RNA polymerase-promoter complex
8. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution
9. Synthesis of the protein cutting reagent iron (S)-1-(p-bromoacetamidobenzyl)ethylenediamine-tetraacetate and conjugation to cysteine side chains
10. Binding of the initiation factor sigma(70) to core RNA polymerase is a multistep process
11. Visualization of RNA tertiary structure by RNA-EDTA-Fe(II) autocleavage: Analysis of tRNAPhe with uridine-EDTA-Fe(II) at position 47
12. Role of sigma subunit of Escherichia coli RNA polymerase initiation. II. Release of sigma from ternary complexes
13. T7 RNA polymerase elongation complex structure and movement
14. Mechanisms by which T7 lysozyme specifically regulates T7 RNA polymerase during different phases of transcription
15. Interactions of the RNA polymerase of bacteriophage T7 with its promoter during binding and initiation of transcription
16. Kinetic mechanism of transcription initiation by bacteriophage T7 RNA polymerase
17. Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme
18. RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes
19. A role for interaction of the RNA polymerase flap domain with the σ subunit in promoter recognition
20. RNA polymerase clamps down
21. Termination and slippage by bacteriophage T7 RNA polymerase
22. Characterization of two types of termination signals for bacteriophage T7 RNA polymerase
23. Processivity in early stages of transcription by T7 RNA polymerase
24. Structural organization of bacterial RNA polymerase holoenzyme and the RNA polymerase-promoter open complex
25. Characterization of bacteriophage T7 RNA polymerase elongation complexes
26. Translocation of 70 with RNA polymerase during transcription: Fluorescence resonance energy transfer assay for movement relative to DNA
27. Transcription elongation: Structural basis and mechanisms
28. Roles of RNA:DNA hybrid stability, RNA structure, and active site conformation in pausing by human RNA polymerase II
29. Studies of contacts between T7 RNA polymerase and its promoter reveals features in common with multisubunit RNA polymerases
30. Model for the mechanism of bacteriophage T7 RNAP transcription initiation and termination
31. Crystal structure of bacteriophage T7 RNA polymerase at 3.3 Å resolution
32. The thumb's knuckle. Flexibility in the thumb subdomain of T7 RNA polymerase is revealed by the structure of a chimeric T7/T3 RNA polymerase
33. A stressed intermediate in the formation of stability initiated RNA chains at the Escherichia coli lac UV5 promoter
34. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes
35. The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance
36. Basic mechanisms of transcript elongation and its regulation
37. Evidence for DNA bending at the T7 RNA polymerase promoter
38. Specificity in transcriptional regulation in the absence of specific DNA binding sites: The case of T7 lysozyme
39. Role of open complex instability in kinetic promoter selection by bacteriophage T7 RNA polymerase
40. Mechanism of inhibition of bacteriophage T7 RNA polymerase by T7 lysozyme
41. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 Å resolution