Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6. Å resolution

Nature

Volumn 417, Issue 6890, 2002, Pages 712-719

  Yokoyama, Shigeyuki ^a,a,c,d   Vassylyeva, Marina N ^a,c  

^a RIKEN HARIMA INSTITUTE   (Japan)

^b SUNY DOWNSTATE MEDICAL CENTER   (United States)

^c RIKEN YOKOHAMA INSTITUTE   (Japan)

^d UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CRYSTAL STRUCTURE; RNA;

TRANSCRIPTION INITIATION;

ENZYMES;

BACTERIAL ENZYME; HOLOENZYME; INITIATION FACTOR; RNA POLYMERASE;

CRYSTAL STRUCTURE; PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; DNA BINDING; ENZYME ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; TRANSCRIPTION INITIATION;

AMINO ACID SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DNA; DNA-BINDING PROTEINS; DNA-DIRECTED RNA POLYMERASES; HOLOENZYMES; MAGNESIUM; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PROMOTER REGIONS (GENETICS); PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SIGMA FACTOR; THERMUS THERMOPHILUS; ZINC FINGERS;

BACTERIA (MICROORGANISMS); THERMUS; THERMUS THERMOPHILUS;

EID: 0037071844     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature752     Document Type: Article

References (48)

1. Prokaryotic and eukaryotic RNA polymerase have homologous core subunits
2. RNA polymerase: Structural similarities between bacterial RNA polymerase and eukaryotic RNA polymerase 11
3. Factors stimulating transcription by RNA polymerase
4. An integrated model of the transcription complex in elongation, termination, and editing
5. 70-retaining transcription elongation complexes from Escherichia coli
6. 70 with RNA polymerase during transcription: Fluorescence resonance energy transfer assay for movement relative to DNA
7. DNA footprints of the two kinetically significant intermediates in formation of an RNA polymerase-promoter open complex: Evidence that interactions with start site and downstream DNA induce sequential conformational changes in polymerase and DNA
8. The functional and regulatory roles of sigma factors in transcription
9. Functional modulation of Escherichia coli RNA polymerase
10. 70 family: Sequence conservation and evolutionary relationships
11. Polypeptides containing highly conserved region of transcription initiation factor Σ 70 exhibit specificity of binding to promoter DNA
12. 70 subunit is responsible for the recognition of the 'extended-10' motif at promoters
13. 70
14. Promoter recognition as measured by binding of polymerase to nontemplate strand oligonudeotide
15. Sigma factor mutations affecting the sequence. Selective interaction of RNA polymerase with-10 region single-stranded DNA
16. The interface of σ with core RNA polymerase is extensive, conserved, and functionally specialized
17. Characterization of the Escherichia coli transcription factor sigma 70: Localization of a region involved in the interaction with core RNA polymerase
18. 70- conjugated chemical protease
19. A crystal structure of Thermus aquaticus core RNA polymerase at 3.3 Å resolution
20. Structural basis of transcription: RNA polymerase II at 28 Å resolution
21. Structural basis of transcription: An RNA polymerase II elongation complex at 3.3 Å resolution
22. A structural model of transcription elongation
23. Structural organization of the RNA polymerase-promoter open complex
24. DNA bending and wrapping around RNA polymerase: A "revolutionary" model describing transcriptional mechanisms
25. Amino-terminal amino acids modulate σ-factor DNA-binding activity
26. Altered promoter recognition by mutant forms of the sigma 70 subunit of Escherichia coli RNA polymerase
27. A mutant Escherichia coli sigma 70 subunit of RNA polymerase with altered promoter specificity
28. Crystal structure of a σ 70 subunit fragment from E. Coli RNA polymerase
29. Protein structure comparison by alignment of distance matrices
30. 70 binding site located on Escherichia coli core RNA polymerase
31. 70
32. A universally conserved region of the largest subunit participates in the active site of RNA polymerase III
33. Structural and functional insights provided by crystal structures of DNA polymerases and their substrate complexes
34. Organization of open complexes at Escherichia coli promoters
35. 70 in an Escherichia coli RNA polymerase-lacUV 5 open promoter complex
36. A "master" in base unpairing during isomerization of a promoter upon RNA polymerase binding
37. 70 within the transcription elongation complex
38. Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus
39. Processing x-ray diffraction data collected in oscillation mode
40. Detecting and overcoming crystal twinning
41. Crystallography and NMR system: A new software suite for macromolecular structure determination
42. Structure at 2.8 Å resolution of F1-ATPase from bovine heart mitochondria
43. Improved methods for binding protein models in electron density maps and the location of errors in these models
44. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
45. Further additions to MolScript version 1.4, including reading and contouring of electron-density maps
46. Raster3D: Photorealistic molecular graphics