Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase

Science

Volumn 298, Issue 5597, 2002, Pages 1387-1395

  Yin, Y Whitney ^a   Steitz, Thomas A ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL STRUCTURE; DNA; ENZYMES; RNA;

ANGSTROM RESOLUTION;

GENETIC ENGINEERING;

DOUBLE STRANDED DNA; MESSENGER RNA; RNA POLYMERASE;

DNA; RNA;

ARTICLE; BACTERIOPHAGE T7; BASE PAIRING; BINDING SITE; COMPLEX FORMATION; CRYSTAL STRUCTURE; DNA BINDING; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN; RNA TRANSCRIPTION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

BACTERIOPHAGE T7; BINDING SITES; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DNA; DNA-DIRECTED RNA POLYMERASES; MODELS, MOLECULAR; MUTATION; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; NUCLEIC ACID HETERODUPLEXES; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; RNA POLYMERASE II; RNA, MESSENGER; TAQ POLYMERASE; TEMPLATES, GENETIC; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

UNIDENTIFIED BACTERIOPHAGE;

EID: 0037112082     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1077464     Document Type: Article

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61. Data were collected at two synchrotron sources for this work ID-19 at Argonne National Labs (APS) and X25 at National Synchrotron Light Source (NSLS). Use of the Argonne National Laboratory Structural Biology Center beamlines at the Advanced Photon Source was supported by the U.S. Department of Energy (DOE), Office of Biological and Environmental Research, under Contract No. W-31-109-ENG-38. Research carried out in part at the NSLS, BNL, was supported by the DOE, Division of Materials Sciences and Division of Chemical Sciences, under Contract No. DE-AC02-98CH10886. We thank M. Becker (X25) and A. Joachimiak (ID-19) for their support during beam[ine data collections; W. Kennedy, C. Joyce, and S. Kamtekar for many helpful discussions and critical reading of the manuscript; and D. Crothers for help with the thermodynamic calculations. The coordinates for the T7 RNAP elongation complex have been deposited in the PDB under accession code 1msw. Supported by NIH grant GMS7510 to T.A.S.